Antinuclear Antibodies (ANA) Profile, 11 biomarkers, by Multiplex Immunoassay, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B, Sm/RNP, Ribosomal P

CPT: 83516; 86225; 86235(x9)
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  • ANA Comprehensive Plus Profile

Test Includes

Centromere B; chromatin; dsDNA; Jo-1; ribosomal P; RNP; scl-70; Sjögren's A; Sjögren's B; Sm/RNP; Smith (Sm)

Special Instructions

Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855].

Expected Turnaround Time

2 - 3 days

Related Documents

Specimen Requirements




1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination; icterus

Test Details


Detect antibodies associated with SLE and mixed connective tissue disease. Sm antibodies are highly specific for SLE. RNP antibodies are found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Autoantibodies to the ribosomal P protein represent a highly specific marker for the diagnosis of SLE, where they have been associated with certain clinical manifestations. The native protein SmRNP contains two different regions: Sm and RNP that are different regions of the same macromolecule. SmRNP is the best antigen for detection of antibodies to either Sm or RNP but cannot discriminate between the antibody specificities. Inclusion of SmRNP increases sensitivity while inclusion of Sm and RNP increases specificity.


Multiplex flow immunoassay


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Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164920 ANA Comprehensive Plus Profile 096343 Anti-DNA (DS) Ab Qn IU/mL 5130-0
164920 ANA Comprehensive Plus Profile 016354 RNP Antibodies AI 29374-6
164920 ANA Comprehensive Plus Profile 016362 Smith Antibodies AI 11090-8
164920 ANA Comprehensive Plus Profile 016376 Smith/RNP Antibodies AI 27048-8
164920 ANA Comprehensive Plus Profile 018707 Antiscleroderma-70 Antibodies AI 27416-7
164920 ANA Comprehensive Plus Profile 012684 Sjogren's Anti-SS-A AI 17792-3
164920 ANA Comprehensive Plus Profile 012692 Sjogren's Anti-SS-B AI 17791-5
164920 ANA Comprehensive Plus Profile 012581 Antichromatin Antibodies AI 51775-5
164920 ANA Comprehensive Plus Profile 012701 Antiribosomal P Antibodies AI 13636-6
164920 ANA Comprehensive Plus Profile 161457 Anti-Jo-1 AI 11565-9
164920 ANA Comprehensive Plus Profile 164816 Anti-Centromere B Antibodies AI 53982-5
164920 ANA Comprehensive Plus Profile 164879 See below: N/A

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