Antinuclear Ab, Qualitative, by Multiplex Immunoassay

CPT: 86038
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  • ANA Direct

Expected Turnaround Time

1 - 3 days

Related Documents

Specimen Requirements




1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination; icterus

Test Details


Detect antibodies to nuclear antigens


Males and females older than 80 years of age have a 50% incidence of low titer ANA. Various medications can induce a “lupoid” condition.


Multiplex flow immunoassay

Reference Interval



Astion ML, Wener MH, Hutchinson K. Autoantibody testing: The use of immunofluorescence and other assays. Clin Lab News. 2000; (7):30-34.
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Eriksson C, Engstrand S, Sundqvist KG, Rantapää-Dahlqvist S. Autoantibody formation in patients with rheumatoid arthritis treated with anti-TNF alpha. Ann Rheum Dis. 2005 Mar; 64(3):403-407. 15297281
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Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med. 2000 Jan; 124(1):71-81. 10629135
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Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164855 Antinuclear Ab by Multiplex 8061-4 164862 ANA Direct 8061-4

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