Histoplasma Antibodies, Quantitative, DID

CPT: 86698
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Test Includes

Antibody titers for mycelial and yeast antigens of Histoplasma

Expected Turnaround Time

2 - 4 days

Related Documents

Specimen Requirements




0.6 mL

Minimum Volume

0.25 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube

Storage Instructions


Stability Requirements




14 days


14 days

Freeze/thaw cycles

Stable x2

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

Test Details


Immunodiffusion test is used to detect antibodies to Histoplasma capsulatum. The antigen used is a culture filtrate. Histoplasmin H and M preciptins can be identified by the assay. The M band often appears first and may occur without the H band. M preciptin is found in about 70% of both acute and chronic histoplasmosis cases. Both M and H occur together in only about 10% of patients. Results should be interpreted in the context of clinical presentation and other laboratory findings (e.g., fungal culture).


Negative fungal serology does not rule out the possibility of current infection.


Double immunodiffusion (DID)

Reference Interval

Negative: <1:1


Heiner DC. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Part 1):616-627.13590840
Palmer D, Kaufman L, Kaplan W, Cavallaro J. Serodiagnosis of Mycotic Diseases. Charles C Thomas, Springfield, IL; 1977.
Reiss E, Kauffman L, Kovacs J, Lindsley M. Clinical Immunomycology, p.559-583. In N. Rose, R. Hamilton, and B. Detrick (ed.), Manual of Clinical Laboratory Immunology. ASM Press, Washington D.C.; 2002.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164319 Histoplasma Abs, Qn, DID 27266-6 164320 Histoplasma Abs, Qn, DID 27266-6

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