Herpes Simplex Virus (HSV) Type 2-Specific Antibodies, IgG With Reflex to Supplemental Testing

CPT: 86696
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  • Herpes-2
  • HSV Type 2-Specific Antibody, IgG
  • HSV-2

Special Instructions

If patient result falls between 0.91 (Equivocal) and 5.00 (Low Positive) Index values, the specimen will reflex to 163006 HSV-2 Supplemental test per CDC guidelines.

If reflex test is performed, additional charges/CPT code(s) may apply.

Expected Turnaround Time

1 - 3 days

Related Documents

Specimen Requirements




1 mL

Minimum Volume

0.5 mL


Red-top tube, gel-barrier tube, or serum transfer tube

Storage Instructions


Stability Requirements



Room temperature

2 days


7 days


98 days

Freeze/Thaw Cycle

Stable x4

Causes for Rejection

Hemolysis; lipemia; grossly icteric; visible particulate matter; gross bacterial contamination

Test Details


Detect IgG antibodies specific to HSV type 2 infection; confirm or rule out possible infection with herpes simplex type 2 virus in prenatal patients in whom HSV 2 infection can cause serious prenatal disease. Per CDC guidelines, specimens with equivocal or low positive results for HSV-2 type specific IgG will reflex for supplemental testing by another assay.

In a patient with no history of lesion disease, a positive result for this test may be indicative that the primary infection was asymptomatic. The magnitude of the Index Value is not indicative of the amount of antibody present in the patient sample. Once infected by HSV, it is possible for a patient to shed virus without the development of lesions (asymptomatic shedding). This test does not indicate the site of HSV infection. There is a considerable homology between HSV-1 and HSV-2 antigens, so that antibodies formed against either virus are highly cross-reactive. This assay is based on glycoprotein G-2, purified from HSV-2 infected cells, and is specific for type 2 antibodies.

HSV-2 IgG Type Specific

HSV-2 IgG Supplemental


Positive / Equivocal


Indicates the presence of detectable IgG antibodies to HSV-2.

Positive / Equivocal


Unable to confirm the presence of IgG antibodies to HSV-2. Recommend retesting in 2 to 4 weeks.


This test is intended for qualitative determination only. The magnitude of the Index Value is not indicative of the amount of antibody present in the patient sample.


Chemiluminescent immunoassay (CLIA). Reflex of specimens equivocal and low positive for HSV-2 type specific IgG to a membrane based immunoassay.

Additional Information

Set up Monday thru Friday.


Ashley R, Cent A, Maggs V, Nahmias A, Corey L. Inability of enzyme immunoassays to discriminate between infections with herpes simplex virus types 1 and 2. Ann Intern Med. 1991 Oct 1; 115(7):520-526.1652909
Centers for Disease Control and Prevention. Sexually-transmitted disease treatment guidelines 2002. MMWR. 2002; 51(RR-6).12184549
Corey L. The current trend in genital herpes. Progress in prevention. Sex Transm Dis. 1994 Mar-Apr; 21(2 Suppl):S38-S44.8042114
Whitley RJ. Herpes simplex infections of women and their offspring: Implications for a developed society. Proc Natl Acad Sci USA. 1994 Mar 29; 91(7):2441-2447 (review).8146137


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
163033 HSV-2 Type Spec Ab, IgG w/Rflx 5209-2 163034 HSV 2 IgG, Type Spec index 5209-2
Reflex Table for HSV 2 IgG, Type Spec
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 000000 163006 000000 163013 HSV-2 IgG Supplemental Test
Reflex 2 043180

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