Test Includes
Amitriptyline/nortriptyline; doxepin/nordoxepin; imipramine/desipramine
Special Instructions
Specify drug ingested.
Expected Turnaround Time
2 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Specimen Requirements
Specimen
Serum or plasma
Volume
2 mL
Minimum Volume
0.6 mL
Container
Red-top tube, lavender-top (EDTA) tube, or green-top (heparin) tube. Do not use a gel-barrier tube. The use of gel-barrier tubes is not recommended due to slow absorption of the drug by the gel. Depending on the specimen volume and storage time, the decrease in drug level due to absorption may be clinically significant.
Collection
Transfer separated serum or plasma to a plastic transport tube.
Storage Instructions
Refrigerate.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 3 days |
Refrigerated | 1 month |
Frozen | 3 months |
Causes for Rejection
Gel-barrier tube
Test Details
Use
Detect the presence of tricyclic antidepressants
Limitations
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Methodology
Liquid chromatography/tandem mass spectrometry (LC/MS-MS)
Reference Interval
Negative (<20 ng/mL)
Additional Information
Tricyclic antidepressants are prescribed for the treatment of endogenous depression and neuralgic pain disorders. The tricyclic antidepressants include amitriptyline, imipramine, doxepin, and their demethylated metabolites, nortriptyline and desipramine. The tricyclic antidepressants block neuronal uptake of norepinephrine and serotonin. The degree of inhibition seems to correlate with antidepressant activity. These drugs also have sedative effects.1
Footnotes
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 007690 | Tricyclic Antidepressants | 10552-8 | 072413 | Amitriptyline | ng/mL | 3333-2 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700088 | Nortriptyline | ng/mL | 3872-9 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700089 | Total (Ami+Nor) | ng/mL | 3335-7 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 072439 | Imipramine | ng/mL | 3690-5 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700104 | Desipramine | ng/mL | 3531-1 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700105 | Total (Imi+Des) | ng/mL | 9627-1 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 072421 | Doxepin | ng/mL | 3579-0 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700096 | Nordoxepin | ng/mL | 3862-0 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700097 | Total Dox+Nordox | ng/mL | 3582-4 |
| 007690 | Tricyclic Antidepressants | 10552-8 | 700472 | Disclaimer: | N/A |
| Reflex Table for Amitriptyline | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Nortriptyline | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Total (Ami+Nor) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Imipramine | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Desipramine | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Total (Imi+Des) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Doxepin | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Nordoxepin | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Total Dox+Nordox | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
| Reflex Table for Disclaimer: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 000000 | |||||
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