Hepatitis C Virus (HCV) Antibody Cascade to Quantitative PCR and Genotyping

CPT: 86803; if reflex test is performed, additional CPT codes may apply.
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Test Details

Use

Diagnosis and management of hepatitis C virus (HCV) infection. A positive antibody test will reflex to a quantitative PCR assay. When the quantitative viral load is >1000 IU/ml an additional reflex to genotyping will occur.

Methodology

Enzyme immunoassay (EIA) with reflex to real-time PCR

Specimen Requirements

Specimen

Serum or plasma

Volume

3 mL

Minimum Volume

1.5 mL

Container

Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT)

Collection

Two samples must be submitted.

Antibody test: If tube other than a gel-barrier tube is used, transfer separated serum/plasma to a plastic transport tube.

PCR test: Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.

Storage Instructions

Antibody test: refrigerated (preferred) up to 14 days. Frozen up to 14 days, room temperature up to 14 days.

PCR test: frozen (preferred) up to 6 weeks, refrigerated up to 72 hours, room temperature up to 24 hours.

Causes for Rejection

Incorrect anticoagulant; PPT not centrifuged

Clinical Information

Special Instructions

Submit two samples, preferably one refrigerated unopened gel-barrier tube and one frozen serum/plasma pour off tube.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
144127 HCV Antibody Cascade(PCR/Geno) 48159-8 144035 HCV Ab s/co ratio 48159-8
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144030 Interpretation: 144030 Interpretation: 13955-0
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 2 000000 550353 000000 IU/mL 11011-4
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 2 000000 550354 000000 log10 IU/mL 38180-6
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550339 HCV log10 log10 IU/mL 38180-6
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550314 Test Information: 19147-8
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550396 Interpretation: N/A
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550394 HCV Genotype N/A
Reflex 2 000000 550512 000000 32286-7 550614
Reflex 3 000000 550614 000000 32286-7
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550553 HCV RT-PCR, Quant (Non-Graph) 550394 HCV Genotype N/A
Reflex 2 000000 550573 000000 N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf