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CPT:
86803; if reflex test is performed, additional CPT codes may apply.
Special Instructions
Submit two samples, preferably one refrigerated unopened gel-barrier tube and one frozen serum/plasma pour off tube.
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Expected Turnaround Time
1 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
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Specimen Requirements
Specimen
Serum or plasma
Volume
3 mL
Minimum Volume
1.5 mL
Container
Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT)
Collection
Two samples must be submitted.
Antibody test: If tube other than a gel-barrier tube is used, transfer separated serum/plasma to a plastic transport tube.
PCR test: Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.
Storage Instructions
Antibody test: refrigerated (preferred)
PCR test: frozen (preferred)
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PCR test: frozen (preferred) up to 6 weeks, refrigerated up to 72 hours, room temperature up to 24 hours. |
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Antibody test: refrigerated (preferred) PCR test: frozen (preferred) |
Stability Requirements
Temperature | Period |
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Room temperature | Antibody test: 14 days PCR test: 24 hours |
Refrigerated | Antibody test: 14 days PCR test: 72 hours |
Frozen | Antibody test: 14 days PCR test: 6 weeks |
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Causes for Rejection
Incorrect anticoagulant; PPT not centrifuged
Test Details
Use
This test is used for diagnosis and management of hepatitis C virus (HCV) infection. A positive antibody test will reflex to a quantitative PCR assay. When the quantitative viral load is >1000 IU/ml an additional reflex to genotyping will occur.
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This test is used for diagnosis and management of hepatitis C virus (HCV) infection. A positive antibody test will reflex to a quantitative PCR assay. When the quantitative viral load is >1000 IU/ml an additional reflex to genotyping will occur. |
Methodology
Enzyme immunoassay (EIA) with reflex to real-time PCR
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 144127 | HCV Antibody Cascade(PCR/Geno) | 40726-2 | 144035 | HCV Ab | 40726-2 |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144030 | Interpretation: | 144030 | Interpretation: | 13955-0 | |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550338 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| Reflex 2 | 550352 | HCV RNA (International Units) | 550353 | HCV RNA (International Units) | IU/mL | 11011-4 |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550338 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| Reflex 2 | 550352 | HCV RNA (International Units) | 550354 | HCV log10 | log10 IU/mL | 38180-6 |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550339 | HCV log10 | log10 IU/mL | 38180-6 |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550314 | Test Information: | 19147-8 | |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550396 | Interpretation: | 62365-2 | |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550394 | HCV Genotype | N/A | |
| Reflex 2 | 550641 | Hepatitis C Genotype | 550512 | Hepatitis C Genotype | 32286-7 | |
| Reflex 3 | 550614 | Hepatitis C Genotype | 550614 | Hepatitis C Genotype | 32286-7 | |
| Reflex Table for HCV Ab | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550553 | HCV RT-PCR, Quant (Non-Graph) | 550394 | HCV Genotype | N/A | |
| Reflex 2 | 550641 | Hepatitis C Genotype | 550573 | Please note: | N/A | |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf