Histoplasma Galactomannan Antigen EIA, Urine

CPT: 87385
Print Share

Test Details

Synonyms

  • Histoplasma Galactomannan Antigen EIA
  • Histoplasma Urinary Antigen

Use

Diagnosis of histoplasmosis based upon detection of antigen

Limitations

A negative result does not preclude a diagnosis of histoplasmosis. This assay has been documented to cross-react with Blastomyces dermatitidis, Coccidioides immitis, Paracoccidioides brasiliensis, and Penicillium marneffei. Positive tests should be confirmed in areas or patient groups where these organisms are endemic or a risk. The following substances may interfere with the assay results: foods which produce color in urine; vaginal cream; caffeine; ascorbic acid, itraconazole; amphotericin B; acetaminophen; and acetylsalicylic acid.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Enzyme immunoassay (EIA)

Reference Interval

Normal value <0.50 ng/mL

Additional Information

Antigen detection may be useful in acute disease, especially in individuals also infected with HIV, who frequently have the disseminated form of histoplasmosis without detectable antibodies to the fungus. In chronic disease, antigen may not be detected due to the low fungal burden. The highest sensitivity for rapidly and accurately diagnosing histoplasmosis in patients with acute pulmonary or disseminated infection can be obtained by testing both urine and serum.

Specimen Requirements

Specimen

Urine (unpreserved)

Volume

5 mL

Minimum Volume

1 mL

Container

Sterile cup or sterile screw-capped vial

Collection

Collect specimen aseptically

Storage Instructions

Refrigerate. Store specimens at 2°C to 8°C or frozen at -16°C to 24°C for up to 14 days.

Causes for Rejection

Specimen not refrigerated or frozen; insufficient specimen volume; inappropriate specimen source; inappropriate specimen collection device (ie, urine collected/submitted in a maintenance fluid or a preservative such as boric acid); specimen stability exceeded; name discrepancy between the name on the specimen container and the name in the computer or on the requisition

Clinical Information

References

ALPHA Histoplasma EIA Test Kit for the Detection of Histoplasma Antigen- REF HAG102, Package Insert, Immuno- Mycologics, Inc., 2700 Technology Place, Norman, OK 73071, Revised 07/18/2011.
Connolly PA., Durkin MM, LeMonte AM, Hackett EJ, Wheat LJ. Detection of Histoplasma antigen by a quantitative enzyme immunoassay. Clin Vaccine Immunol. 2007 Dec;14(12):1587-1591. 17913863
Cox M, Pesek G, Phan D, Woods G. Utility of a Histoplasma capsulatum enzyme immunoassay for diagnosis of disseminated histoplasmosis and correlation with disease activity. Arch Pathol Lab Med. 2008. 132:1512.
Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st Century, Clin Microbiol Rev. 2011, Apr; 24(2):247-280.21482725
Guimaraes AJ, Nosanchuk JD, Zancope-Oliverira RM. Diagnosis of histoplasmosis. Braz J. Microbiol. 2006 Jan;37(1):1-13.20445761
Kauffman CA.. Histoplasmosis: A Clinical and Laboratory Update. Clinical Microbiology Reviews. 2007 Jan;20(1):115-132.17223625
Wheat LJ, Connolly-Stringfield PA, Baker RL, et al. Disseminated histoplasmosis in the acquired immune deficiency syndrome: clinical findings, diagnosis and treatment, and review of the literature. Medicine (Baltimore) 1990 Nov;69(6):361-374.2233233
Wheat LJ, Freifeld AG, Kleiman MB, et al. Clinical practice guidelines for the management of patients with histoplasmosis: 2007 update by the Infectious Diseases Society of America. Clin Infect Dis. 2007 Oct 1;45(7):807-825.17806045
Wheat LJ, Kohler RB, Tewari RP. Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986 Jan 9;314(2):83-88.3941695
Zhang X, Gibson B Jr, Daly TM. Evaluation of commercially available reagents for diagnosis of histoplasmosis infection in immunocompromised patients. J Clin Microbiol 2013 Dec;51(12):4095-4101.24088857

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183560 Histoplasma Gal'mannan Ag Ur 48952-6 183562 Histoplasma Gal'mannan Ag Ur 44524-7
183560 Histoplasma Gal'mannan Ag Ur 48952-6 164013 Disclaimer: N/A
Reflex Table for Histoplasma Gal'mannan Ag Ur
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183563 Histoplasma Gal'mannan Ag Qn U 183563 Histoplasma Gal'mannan Ag Qn U ng/mL 48952-6

For Providers

Please login to order a test.

 

© 2018  Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf