West Nile Virus (WNV), UltraQual® RT-PCR

CPT: 87798
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Test Details


  • WNV, UltraQual® RT-PCR


Detect West Nile virus (WNV) RNA


• Mean detection: 7.43 copies/mL

• 95% detection cutoff: 28.16 copies/mL


UltraQual™ polymerase chain reaction (PCR)

Additional Information

The direct detection of WNV is a valuable tool in determining whether a patient is infected with West Nile virus. It is also useful in determining whether blood and blood products are free of detectable WNV prior to distribution. PCR is able to detect WNV RNA directly and does not rely on antibody markers of WNV infection, which are produced after RNA is detectable in newly infected individuals.

Specimen Requirements


Plasma, serum, or cerebrospinal fluid (CSF)


3.5 mL

Minimum Volume

2.5 mL (Note: 1.25 mL required per isolation. This volume does not allow for repeat testing.)


Frozen transport tube or plasma preparation tube (PPT™)


Collect blood in gel-barrier tube, yellow-top (ACD) tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™). Centrifuge blood at 800−1600xg at room temperature within one hour of draw to separate serum or plasma from cells. Transfer serum or plasma to a plastic screw-capped transport tube and freeze. Specimens drawn into PPT™ need to be centrifuged in a swinging bucket rotor centrifuge within one hour and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions


Clinical Information


Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr; 162(1):156-159.2440339
Kawasaki ES. Amplification of RNA. In Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, Calif: Academic Press;1990:21-28.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140005 NGI West Nile Virus RT PCR 140006 NGI West Nile Virus RT PCR 34892-0
140005 NGI West Nile Virus RT PCR 140008 PCR Amplification + Detection N/A

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