West Nile Virus (WNV), UltraQual® RT-PCR

CPT: 87798
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Test Details

Synonyms

  • WNV, UltraQual® RT-PCR

Use

Detect West Nile virus (WNV) RNA

Limitations

• Mean detection: 7.43 copies/mL

• 95% detection cutoff: 28.16 copies/mL

Methodology

UltraQual™ polymerase chain reaction (PCR)

Additional Information

The direct detection of WNV is a valuable tool in determining whether a patient is infected with West Nile virus. It is also useful in determining whether blood and blood products are free of detectable WNV prior to distribution. PCR is able to detect WNV RNA directly and does not rely on antibody markers of WNV infection, which are produced after RNA is detectable in newly infected individuals.

Specimen Requirements

Specimen

Plasma, serum, or cerebrospinal fluid (CSF)

Volume

3.5 mL

Minimum Volume

2.5 mL (Note: 1.25 mL required per isolation. This volume does not allow for repeat testing.)

Container

Frozen transport tube or plasma preparation tube (PPT™)

Collection

Collect blood in gel-barrier tube, yellow-top (ACD) tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™). Centrifuge blood at 800−1600xg at room temperature within one hour of draw to separate serum or plasma from cells. Transfer serum or plasma to a plastic screw-capped transport tube and freeze. Specimens drawn into PPT™ need to be centrifuged in a swinging bucket rotor centrifuge within one hour and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze

Clinical Information

References

Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr; 162(1):156-159.2440339
Kawasaki ES. Amplification of RNA. In Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, Calif: Academic Press;1990:21-28.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140005 NGI West Nile Virus RT PCR 140006 NGI West Nile Virus RT PCR 34892-0
140005 NGI West Nile Virus RT PCR 140008 PCR Amplification + Detection N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf