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25-Hydroxy Vitamin D (D2+D3 Fractionated), LC/MS-MS
- Vitamin D, 25-Hydroxy, LC/MS-MS
Liquid chromatography/tandem mass spectrometry (LC/MS-MS)
The biological function of vitamin D is to maintain normal levels of calcium and phosphorus absorption. 25-Hydroxy vitamin D is the storage form of vitamin D. Vitamin D assists in maintaining bone health by facilitating calcium absorption. Vitamin D deficiency can also cause osteomalacia, which frequently affects elderly patients. Vitamin D from sunshine on the skin or from dietary intake is converted predominantly by the liver into 25-hydroxy vitamin D, which has a long half-life and is stored in the adipose tissue. The metabolically active form of vitamin D, 1,25-di-hydroxy vitamin D, which has a short life, is then synthesized in the kidney as needed from circulating 25-hydroxy vitamin D. The reference interval of greater than 30 ng/mL is a target value established by the Endocrine Society.2
Serum (preferred) or plasma
0.5 mL (Note: This volume does not allow for repeat testing.)
Serum or plasma must be separated from cells within 45 minutes of venipuncture. Submit serum or plasma in a plastic transport tube.
Freeze. Stable frozen for 34 months. Stable at room temperature or refrigerated for seven days.