Human Papillomavirus (HPV), Biopsy in situ Hybridization Low- and High-risk Subtypes

TEST: 503500
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CPT: 88364(x2); 88365
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Synonyms

  • HPV by ISH

Expected Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen

Tissue

Volume

One formalin-fixed, paraffin embedded (FFPE) tissue block or eight unstained positively charged slides sectioned from FFPE tissue block at 4 to 5 microns

Minimum Volume

Five unstained tissue slides

Container

Paraffin block transport pouch or slide container

Collection

Tissue biopsy should be fixed in 10% neutral buffered formalin for no more than 72 hours. Embed in paraffin.

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Insufficient tissue; inappropriate tissue source; incorrect specimen type

Test Details

Use

Determine HPV type; confirm HPV infection; resolve cases with equivocal histopathology

Limitations

More than 20 distinct HPV types have been isolated from the anogenital mucosa. This assay can detect the presence of six specific viral types: HPV 6, 11, 16, 18, 31, and 33.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

DNA probe; in situ hybridization

References

Anderson SM, Brooke PK, Van Eyck SL, Noell H, Frable WJ. Distribution of human papillomavirus types in genital lesions for two temporarily distinct populations determined by in situ Hybridization. Hum Pathol. 1993 May;24(5):547-553.8387959
Nuovo GJ, Darfler MM, Impraim CC, Bromely SE. Occurrence of multiple types of human papillomavirus in genital tract lesions: analysis by in situ Hybridization and the polymerase chain reaction. Am J Pathol. 1991 Jan;138(1):53-58.1846265

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
503500 HPV ISH Low/High Risk Subtypes 503502 HPV 6/11 18480-4
503500 HPV ISH Low/High Risk Subtypes 503586 HPV 16/18 18478-8
503500 HPV ISH Low/High Risk Subtypes 503587 HPV 31/33 81439-2
503500 HPV ISH Low/High Risk Subtypes 503596 Comment: 77202-0
503500 HPV ISH Low/High Risk Subtypes 503588 Director Review 72486-4

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