Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

CPT: 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Test Details


  • HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay
  • HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay

Test Includes

Reflex to HCV NS5A drug resistance assay if viral load is ≥500 IU/mL.


Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.


The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.


Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) NS5A Drug Resistance Assay [550325] for more information.

Specimen Requirements


Plasma (preferred) or serum, frozen


4 mL

Minimum Volume

2 mL


Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.


Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions


Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number (or unique identifier) to order serial monitoring.


Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

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