Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A

CPT: 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Test Details

Synonyms

  • HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A
  • HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A

Test Includes

Reflex to HCV GenoSure® NS3/4A if viral load is ≥2000 IU/mL

Use

Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.

Limitations

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

Methodology

Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) GenoSure® NS3/4A [550540] for more information.

Specimen Requirements

Specimen

Plasma (preferred) or serum, frozen

Volume

4 mL

Minimum Volume

2 mL

Container

Lavender-top (EDTA) tube, Plasma Preparation Tube (PPT™), gel-barrier tube

Collection

Collect specimen in two PPT™ tubes, gel-barrier tubes or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number (or unique identifier) to order serial monitoring.

References

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550066 HCV RNA Qn (Graph) Rfx NS3/4A 550073 Hepatitis C Quantitation IU/mL 11011-4
550066 HCV RNA Qn (Graph) Rfx NS3/4A 550074 HCV log10 log10 IU/mL 38180-6
550066 HCV RNA Qn (Graph) Rfx NS3/4A 550308 Test Information: N/A
550066 HCV RNA Qn (Graph) Rfx NS3/4A 550082 HCV GenoSure(R) NS3/4A N/A
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550078 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550081 HCV log10 log10 IU/mL 38180-6
Reflex Table for HCV GenoSure(R) NS3/4A
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550538 Rfx to GenoSure(R) HCV NS3/4A 550544 HCV GenoSure(R) NS3/4A PDF 32286-7
Reflex Table for HCV GenoSure(R) NS3/4A
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550538 Rfx to GenoSure(R) HCV NS3/4A 550545 HCV GenoSure(R) NS3/4A 32286-7
Reflex Table for HCV GenoSure(R) NS3/4A
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550538 Rfx to GenoSure(R) HCV NS3/4A 550546 HCV GenoSure(R) NS3/4A Interp 77202-0

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