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Hepatitis C Virus (HCV) Genotype 3 NS5A Drug Resistance Assay
- NS5A Resistance Analysis
Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Assay should be used for patients with documented HCV genotype 3.
This procedure may not be successful when the HCV viral load is <500 IU/mL. This assay requires an HCV genotype of 3.
Polymerase chain reaction (PCR) amplification and DNA next generation sequencing (NGS)
Plasma (preferred) or serum, frozen
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube
Please confirm the patient's HCV genotype 3 prior to submitting sample for testing.