Hepatitis C Virus (HCV) Antibody With Reflex for HCV Antibody Verification

CPT: 86803. If reflex testing is performed, concomitant CPT codes/charges will apply.
Updated on 11/29/2017
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Test Details

Synonyms

  • Anti-HCV Reflex

Use

Since as many as 90% of commercial intravenous immunoglobulins test positive for hepatitis C antibody, an artifactual positive can result briefly after transfusion. Following the development of sensitive and specific testing for hepatitis B, 90% of post-transfusion hepatitis is now hepatitis C. A gene product (c100) of hepatitis C virus (HCV) was isolated and an assay for anti-HCV developed. The assay detects antibody to presumptive togavirus which may be an etiologic agent of non-A, non-B hepatitis (which may not be a unitary disease entity).

Limitations

Testing for HCV antibodies is to assess past or present exposure to hepatitis C virus infection; additional testing for the hepatitis virus is required to determine acute or active infection.

Methodology

Enzyme immunoassay (EIA) reflex to electrochemiluminescence (ECLIA)

Specimen Requirements

Specimen

Serum or plasma

Volume

1 mL

Container

Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube

Collection

If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Non-EDTA plasma specimen; PST gel-barrier tube

Clinical Information

Special Instructions

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

References

Choo QL, Kuo G, Weiner AJ, et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989; 244(4902):359-362. 2523562
Di Bisceglie AM. Hepatitis C and hepatocellular carcinoma. Hepatology., 1997; 26(3 Suppl 1):34S-38S.9305661
Hepatitis C Past and Present. 2011 Case Definition. CDC CSTE Case Definition, Number 10-ID-09.
Koff RS, Dienstage JL. Extrahepatic manifestations of hepatitis C and the association with alcoholic liver disease. Semin Liver Dis. 1995; 15(1):101-109.7597440
Roche Anti-HCV. Package insert 2011-01.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
144065 HCV Ab w/Rflx to Verification 48159-8 144066 HCV Ab s/co ratio 48159-8
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144067 Comment: 144067 Comment: N/A
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144069 Comment: 144069 Comment: N/A
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144075 HCV Ab Verification 144076 HCV Antibody Verification 13955-0
Reflex 2 000000 144080 000000 N/A
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144075 HCV Ab Verification 144076 HCV Antibody Verification 13955-0
Reflex 2 000000 144078 000000 N/A
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144075 HCV Ab Verification 144076 HCV Antibody Verification 13955-0
Reflex 2 000000 144079 000000 N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf