Hepatitis C Virus (HCV) Antibody

CPT: 86803
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Test Details


Assess exposure to hepatitis C virus infection; test blood safety


Since as many as 90% of commercial intravenous immunoglobulins test positive for hepatitis C antibody, an artifactual positive can result briefly after transfusion.


Enzyme immunoassay (EIA)

Additional Information

Following the development of sensitive and specific testing for hepatitis B, 90% of post-transfusion hepatitis is now hepatitis C. A gene product (c100) of hepatitis C virus (HCV) was isolated and an assay for anti-HCV developed. The assay detects antibody to a presumptive togavirus or flavivirus which may be an etiologic agent of non-A, non-B hepatitis (which may not be a unitary disease entity).

For blood donors, hepatitis C serology correlates with surrogate tests for non-A, non-B hepatitis (ALT and anti-HBc). Since hepatitis C serology identifies a broader group of infected individuals than surrogate testing, it reduces risk of HCV during transfusion. Studies in hemophiliacs indicate that antibody to HCV is a reliable marker of HCV.

Specimen Requirements


Serum or plasma


1 mL


Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube


If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Non-EDTA plasma specimen; PST gel-barrier tube

Clinical Information

Special Instructions

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.


Allain JP, Dailey SH, Laurain Y, et al. Evidence for persistent hepatitis C virus (HCV) infection in hemophiliacs. J Clin Invest. 1991 Nov; 88(5):1672-1679. 1939652
Alter MJ, Hadler SC, Judson FN, et al. Risk factors for acute non-A, non-B hepatitis in the United States and association with hepatitis C virus infection. JAMA. 1990 Nov 7; 264(17):2231-2235. 2170702
Choo QL, Kuo G, Weiner AJ, et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989 Apr 21; 244(4902):359-362. 2523562
Clemens JM, Taskar S, Chau K, et al. IgM antibody response in acute hepatitis C viral infection. Blood. 1992 Jan 1; 79(1):169-172. 1309424
Dodd LG, McBride JH, Gitnick GL, Howanitz PJ, Rodgerson DO. Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in human immunoglobulins. Am J Clin Pathol. 1992 Jan; 97(1):108-113. 1728850
Gambino R. NANB hepatitis−A new antibody test for the hepatitis C virus. Lab Report for Physicians. 1988; 10:89-93.
Kuo G, Choo QL, Alter HJ, et al. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21; 244(4902):362-364. 2496467
Richards C, Holland P, Kuramoto K, Douville C, Randell R. Prevalence of antibody to hepatitis C virus in a blood donor population. Transfusion. 1991 Feb; 31(2):109-113.1847559


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140659 HCV Antibody 48159-8 140689 Hep C Virus Ab s/co ratio 48159-8

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