Hepatitis B Surface Antibody, Quantitative

CPT: 86317
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Test Details


  • Anti-HBs
  • Antibody to Hepatitis B Surface Antigen


Hepatitis B surface antibody (anti-HBs) level can be measured to determine if vaccination is needed. Following a vaccination regimen, anti-HBs can be measured to determine if protective immunity has been achieved.


Presence of anti-HBs is not an absolute indicator of resolved hepatitis infection nor of protection from future infection. Since there are different serologic subtypes of hepatitis B virus, it is possible (and has been reported) for a patient to have antibody to one surface antigen type and to be acutely infected with a virus of a different subtype. Thus, a patient may have coexisting HBsAg and anti-HBs. Transfused individuals or hemophiliacs receiving plasma components may give false-positive tests for antibody to hepatitis B surface antigen.


Immunochemiluminometric assay (ICMA)

Reference Interval

• Inconsistent with immunity: 0.0−9.9 mIU/mL

• Consistent with immunity: >9.9 mIU/mL

Specimen Requirements


Serum or plasma


1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube


If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Non-EDTA plasma specimen; PST gel-barrier tube

Clinical Information


Devine P, Taswell HF, Moore SB, Gilchrist GS, Jacobson TM. Passively acquired antibody to hepatitis B surface antigen. Pitfall in evaluating immunity to hepatitis B viral infections. Arch Pathol Lab Med. 1989 May; 113(5):529-531. 2712676
Edwards MS. Hepatitis B serology—Help in interpretation. Pediatr Clin North Am. 1988; 35(3):503-515 (review). 3287312
Foutch PG, Carey WD, Tabor E, et al. Concomitant hepatitis B surface antigen and antibody in 13 patients. Ann Intern Med. 1983 Oct; 99(4):460-463. 6625377
Francis DP, Hadler SC, Thompson SE, et al. The prevention of hepatitis B with vaccine: Report of the Centers for Disease Control multi-center efficacy trial among homosexual men. Ann Intern Med. 1982 Sep; 97(3):362-366. 6810736
Lee HS, Vyas GN. Diagnosis of viral hepatitis. Clin Lab Med. 1987 Dec; 7(4):741-757. 3319367
Protection against viral hepatitis. Recommendations of the Immunization Practices Advisory Committee (ACIP). MMWR Recomm Rep. 1990 Feb 9; 39(RR-2):1-26. 2153904
Swenson PD, Escobar MR, Carithers RL Jr, Sobieski TJ 3rd. Failure of preexisting antibody against hepatitis B surface antigen to prevent subsequent hepatitis B infection. J Clin Microbiol. 1983 Aug; 18(2):305-309. 6619284


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006530 Hepatitis B Surf Ab Quant 16935-9 006531 Hepatitis B Surf Ab Quant mIU/mL 16935-9

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