Hemoglobinopathy Fractionation Profile

CPT: 83021; 85660
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Test Details

Synonyms

  • Hb Electrophoresis Plus Solubility

Test Includes

Identification and relative percentages of hemoglobins (A, A2, F, S, C) including variants, if present; hemoglobin solubility; interpretation

Use

Diagnose thalassemias and hemoglobin variants

Methodology

Hemoglobin fractionation: high-pressure liquid chromatography (HPLC); hemoglobin solubility: sodium hydrosulfite reduction

Reference Interval

• Hb A:

– <7 months: Not established

– 7 months to 1 year: 94.6% to 98.5%

– ≥2 years: 96.4% to 98.8%

• Hb F:

− <7 months: Not established

− 7 months to 1 year: 0.1% to 6.8%

− ≥2 years : 0.0% to 2.0%

• Hb S, D, G: 0%

• Hb C, E, O: 0%

• Hb A2:

– <7 months: Not established

– 7 months to 1 year: 1.9% to 2.8%

– ≥2 years: 1.8% to 3.2%

• Hemoglobin solubility: Negative

Specimen Requirements

Specimen

Whole blood

Volume

1 mL

Minimum Volume

0.6 mL

Container

Lavender-top (EDTA) tube

Collection

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.

Storage Instructions

Refrigerate at 2°C to 8°C.

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

State patient's age, ethnic background, MCV, transfusion history, and any other known hematological information on the test request form.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121679 Hgb Frac. Profile 121680 Hgb Solubility 6864-3
121679 Hgb Frac. Profile 121685 Hgb F % 4576-5
121679 Hgb Frac. Profile 121681 Hgb A % 4546-8
121679 Hgb Frac. Profile 121682 Hgb S % 4625-0
121679 Hgb Frac. Profile 121683 Hgb C % 4563-3
121679 Hgb Frac. Profile 121684 Hgb A2 % 34660-1
121679 Hgb Frac. Profile 121686 Hgb Variant % 42248-5
121679 Hgb Frac. Profile 121687 Interpretation 12710-0
Reflex Table for Interpretation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121088 LCA Hemoglobin Lab INTERP 121089 LCA Hemoglobin Lab INTERP 12710-0

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The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf