Hemoglobinopathy Fractionation Profile

CPT: 83021; 85660
Updated on 5/28/2019
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Synonyms

  • Hb Electrophoresis Plus Solubility

Test Includes

Identification and relative percentages of hemoglobins (A, A2, F, S, C) including variants, if present; hemoglobin solubility; interpretation


Special Instructions

State patient's age, ethnic background, MCV, transfusion history, and any other known hematological information on the test request form.


Expected Turnaround Time

2 - 5 days

2 - 5 days


Related Documents


Specimen Requirements


Specimen

Whole blood


Volume

1 mL


Minimum Volume

0.6 mL


Container

Lavender-top (EDTA) tube


Collection

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.


Storage Instructions

Refrigerate at 2°C to 8°C.


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Patient Preparation

Fasting or fluid restriction is not necessary.

Fasting or fluid restriction is not necessary.


Causes for Rejection

Clotted specimen

Clotted specimen


Test Details


Use

Diagnose thalassemias and hemoglobin variants


Methodology

Hemoglobin fractionation: high-pressure liquid chromatography (HPLC); hemoglobin solubility: sodium hydrosulfite reduction


Reference Interval

• Hb A:

– <7 months: Not established

– 7 months to 1 year: 94.6% to 98.5%

– ≥2 years: 96.4% to 98.8%

• Hb F:

− <7 months: Not established

− 7 months to 1 year: 0.1% to 6.8%

− ≥2 years : 0.0% to 2.0%

• Hb S, D, G: 0%

• Hb C, E, O: 0%

• Hb A2:

– <7 months: Not established

– 7 months to 1 year: 1.9% to 2.8%

– ≥2 years: 1.8% to 3.2%

• Hemoglobin solubility: Negative


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121679 Hgb Frac. Profile 121680 Hgb Solubility 6864-3
121679 Hgb Frac. Profile 121681 Hgb A % 4546-8
121679 Hgb Frac. Profile 121682 Hgb S % 4625-0
121679 Hgb Frac. Profile 121683 Hgb C % 4563-3
121679 Hgb Frac. Profile 121684 Hgb A2 % 34660-1
121679 Hgb Frac. Profile 121685 Hgb F % 4576-5
121679 Hgb Frac. Profile 121686 Hgb Variant % 42248-5
121679 Hgb Frac. Profile 121687 Interpretation 12710-0
Reflex Table for Interpretation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121088 LCA Hemoglobin Lab INTERP 121089 LCA Hemoglobin Lab INTERP 12710-0

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