Hemoglobinopathy Fractionation Profile

CPT: 83021; 85660
Updated on 5/28/2019
Print Share


  • Hb Electrophoresis Plus Solubility

Test Includes

Identification and relative percentages of hemoglobins (A, A2, F, S, C) including variants, if present; hemoglobin solubility; interpretation

Special Instructions

State patient's age, ethnic background, MCV, transfusion history, and any other known hematological information on the test request form.

Expected Turnaround Time

2 - 5 days

2 - 5 days

Related Documents

Specimen Requirements


Whole blood


1 mL

Minimum Volume

0.6 mL


Lavender-top (EDTA) tube


To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.

Storage Instructions

Refrigerate at 2°C to 8°C.

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Patient Preparation

Fasting or fluid restriction is not necessary.

Fasting or fluid restriction is not necessary.

Causes for Rejection

Clotted specimen

Clotted specimen

Test Details


Diagnose thalassemias and hemoglobin variants


Hemoglobin fractionation: high-pressure liquid chromatography (HPLC); hemoglobin solubility: sodium hydrosulfite reduction

Reference Interval

• Hb A:

– <7 months: Not established

– 7 months to 1 year: 94.6% to 98.5%

– ≥2 years: 96.4% to 98.8%

• Hb F:

− <7 months: Not established

− 7 months to 1 year: 0.1% to 6.8%

− ≥2 years : 0.0% to 2.0%

• Hb S, D, G: 0%

• Hb C, E, O: 0%

• Hb A2:

– <7 months: Not established

– 7 months to 1 year: 1.9% to 2.8%

– ≥2 years: 1.8% to 3.2%

• Hemoglobin solubility: Negative


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121679 Hgb Frac. Profile 121680 Hgb Solubility 6864-3
121679 Hgb Frac. Profile 121681 Hgb A % 4546-8
121679 Hgb Frac. Profile 121682 Hgb S % 4625-0
121679 Hgb Frac. Profile 121683 Hgb C % 4563-3
121679 Hgb Frac. Profile 121684 Hgb A2 % 34660-1
121679 Hgb Frac. Profile 121685 Hgb F % 4576-5
121679 Hgb Frac. Profile 121686 Hgb Variant % 42248-5
121679 Hgb Frac. Profile 121687 Interpretation 12710-0
Reflex Table for Interpretation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121088 LCA Hemoglobin Lab INTERP 121089 LCA Hemoglobin Lab INTERP 12710-0

For Providers

Please login to order a test.


© 2019  Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf