Hematopathology Consultation, Peripheral Smear

Whole blood and peripheral blood films made at the time of collection

Tube filled to capacity and two freshly prepared blood films

0.5 mL (500 μL for pediatric microtainer capillary tubes; fill tube to capacity.) (Note: This volume does not allow for repeat testing.)

Lavender-top (EDTA) tube and glass slides

Guidelines for clinician who has prior knowledge of patient history and abnormal labs:

• Hemolytic anemia, especially if accompanied by thrombocytopenia

• Anemia unresponsive to therapy; unexplained anemia

• Leukocyte/bone marrow disorder − pancytopenia, unexplained leukocytosis or leukopenia; leukemia

• Congenital disorders

Clinician suspects from patient history or previous abnormal labs:

• Thrombotic thrombocytopenia purpura (TTP)

• Hemolytic uremic syndrome (HUS) or other unspecified hemolytic anemia

• Leukocyte/bone marrow disorder − pancytopenia, unexplained leukocytosis or leukopenia; leukemia

• Congenital disorders

Determine qualitative and quantitative variations in white cell numbers and morphology, morphology of red cells and platelet evaluation; evaluate anemia, leukemia, and other conditions.

Microscopic examination