EGFR Oncology FISH

CPT: 88271(x2); 88275
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Test Details

Synonyms

  • Brain Cancer
  • Breast Cancer
  • Lung Cancer

Use

Confirmation/identification of cancer-related alterations (for lung and brain cancer)

Limitations

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Fluorescence in situ hybridization (FISH)

Additional Information

High EGFR copy number or ratio of EGFR:control >2.0 detected by FISH has been shown to correlate with response, progression-free survival, and overall survival after treatment with EGFR TKIs in patients with non−small-cell lung cancer (NSCLC).1

Specimen Requirements

Specimen

Three slides of 4- or 5-micron FFPE sections with tumor. Blood or paraffin block (acceptable at an additional charge).

Volume

Three slides of 4- or 5-micron thick with tumor or 10 mL blood (adult), 5 mL blood (pediatric)

Minimum Volume

Two slides of 4- or 5-micron thick with tumor or 5 mL blood (adult), 1 mL blood (pediatric)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal

Collection

Transport to the testing lab at room temperature; use a cool pack or LabCorp transport kit. Do not allow sample to freeze.

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Broken or dirty slides; excessive cellular debris or stained slides; decalcified bone cores; frozen specimen; no tumor submitted

Clinical Information

Special Instructions

There will be an additional charge for processing paraffin block samples or for samples that require culture.

Footnotes

1. Cappuzzo F, Hirsch FR, Rossi E, et al. Epidermal growth factor receptor gene and protein and gefitinib sensitivity in non-small-cell lung cancer. J Natl Cancer Inst 2005 May 4; 97(9):643-655.17317677

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
510355 EGFR Oncology FISH 511736 Specimen Type 31208-2
510355 EGFR Oncology FISH 511737 Cells Counted 62361-1
510355 EGFR Oncology FISH 511738 Cells Analyzed 62360-3
510355 EGFR Oncology FISH 510353 FISH Result 48674-6
510355 EGFR Oncology FISH 511739 Interpretation 62365-2
510355 EGFR Oncology FISH 510356 Director Review: 48672-0
510355 EGFR Oncology FISH 510239 EGFR FISH, Putaway N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf