Chromosome Analysis, High Resolution

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Test Details

Synonyms

  • High Resolution G-Banding
  • Prophase Analysis

Test Includes

20 prophase to prometaphase cells are studied following cell synchronization. Two cells are karyotyped with the highest resolution.

Use

Detect small chromosome abnormalities not detectable using routine methods; precise identification of abnormal chromosomes previously detected by routine methods

Methodology

Culture of cells in special medium; colcemid arrest of cells in prometaphase stage of mitosis following cell synchronization techniques

Specimen Requirements

Specimen

Whole blood

Volume

10 mL (adult), 2 mL (pediatric)

Minimum Volume

1 mL

Container

Green-top (heparin) tube

Collection

Using sterile technique, collect 10 mL of blood into a sodium heparin tube or syringe. Invert the tube several times to prevent coagulation. Specimens must be sent the same day as collected and arrive in the laboratory within 48 hours of collection.

Storage Instructions

Specimen should be delivered immediately. Maintain specimen at room temperature. Specimen may be refrigerated if there is a delay in shipment. Do not freeze.

Causes for Rejection

Hemolysis; clotted specimen; use of improper anticoagulant; frozen specimen

Clinical Information

Special Instructions

Pertinent medical findings must accompany request for chromosome analysis.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
052215 Chromosome, High Resolution 052107 Cells Counted 62361-1
052215 Chromosome, High Resolution 052108 Cells Karyotyped 55199-4
052215 Chromosome, High Resolution 052109 Cells Analyzed 62360-3
052215 Chromosome, High Resolution 052110 GTG Band Resolution Achieved 62358-7
052215 Chromosome, High Resolution 052008 Cytogenetic Diagnosis 48818-9
052215 Chromosome, High Resolution 052112 Cytogenetic Interpretation: 50619-6
052215 Chromosome, High Resolution 052113 Specimen Type 31208-2
052215 Chromosome, High Resolution 052009 Director Review: 48672-0
052215 Chromosome, High Resolution 511936 PDF 80563-0

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