Celiac Antibodies tTG IgA/IgG With DGP IgA/IgG Positive/Negative Combination Screen

CPT: 83516
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Test Details

Synonyms

  • tTG/DGP Screen

Test Includes

IgA and IgG antibodies to deamidated gliadin-derived peptides (DGP) and human tissue transglutaminase (h-tTG) in human serum

Use

For detection of IgA and IgG antibodies to deamidated gliadin-derived peptides (DGP) and human tissue transglutaminase (h-tTG) in human serum. The presence of these antibodies can be used in conjunction with clinical findings and other laboratory tests to aid in the diagnosis of IgA-sufficient and IgA-deficient celiac disease.

Methodology

Enzyme-linked immunosorbent assay (ELISA)

Specimen Requirements

Specimen

Serum

Volume

0.5 mL

Minimum Volume

0.25 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection

Serum separated from clot

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Causes for Rejection

Microbially-contaminated, heat-treated, or specimens containing visible particulate; grossly hemolyzed or lipemic serum specimens

Clinical Information

References

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Andersson AS, Holtz A, Backhans B, et al. Inhibition of endomysial staining with human tissue transglutaminase. Ninth International Symposium on Celiac Disease. J Pediatr Gastroenterol Nutr. 2000; 31(S16).
Axiö-Frekricksson UB. Differences in human and guinea pig tissue transglutaminase. Ninth International Symposium on Celiac Disease. J Pediatr Gastroenterol Nutr. 2000; 31(S16).
Bürgin-Wolff A, Gaze H, Hadziselimovic F, et al. Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. 1991 Aug; 66(8):941-947. 1819255
Centers for Disease Control and Prevention and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories. 5th ed. 2007.
CLSI (NCCLS). Procedures for the Handling and Processing of Blood Specimens; Approved Guideline--Third Edition. CLSI Document H18-A3, 2004, 24(38).
CLSI (NCCLS). Statistical Quality Control for Quantitative Measurement Procedures: Principles and Definitions; Approved Guideline--Third Edition. NCCLS Document C24-A3, 2006, 26(25).
Collin P, Mäki M, Keyriläinen O, Hällström O, Reunala T, Pasternack A. Selective IgA deficiency and coeliac disease. Scand J Gastroenterol. 1992 May; 27(5):367-371.1529270
Dieterich W, Ehnis T, Bauer M, et al. Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med. 1997 Jul; 3(7):797-801.9212111
Hansson T, Dahlbom I, Hall J, et al. Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease. J Pediatr Gastroenterol Nutr. 2000 Apr; 30(4):379-384.10776947
Lampasona V, Bazzigaluppi E, Barera G, Bonifacio E. Tissue transglutaminase and combined screening for coeliac disease and type 1 diabetes-associated autoantibodies.Lancet. 1998 Oct 10; 352(9135):1192-1193.9777840
Lindh E, Ljunghall S, Larsson K, Lavö B. Screening for antibodies against gliadin in patients with osteoporosis. J Intern Med. 1992 Apr; 231(4):403-406.1588266
McMillan SA, Haughton DJ, Biggart JD, Edgar JD, Porter KG, McNeill TA. Predictive value for coeliac disease of antibodies to gliadin, endomysium, and jejunum in patients attending for jejunal biopsy. BMJ. 1991 Nov 9; 303(6811):1163-1165.1747612
Osman AA, Günnel T, Dietl A, et al. B cell epitopes of gliadin. Clin Exp Immunol. 2000 Aug; 121(2):248-254.10931138
Prince HE. Evaluation of the INOVA diagnostics enzyme-linked immunosorbent assay kits for measuring serum immunoglobulin G (IgG) and IgA to deamidated gliadin peptides. Clin Vaccine Immunol. 2006 Jan; 13(1):150-151.16426013
Revised criteria for diagnosis of coeliac disease. Report of Working Group of European Society of Paediatric Gastroenterology and Nutrition. Arch Dis Child. 1990; 65(8):909-911.2205160
Sárdy M, Odenthal U, Kárpáti S, Paulsson M, Smyth N. Recombinant human tissue transglutaminase ELISA for the diagnosis of gluten-sensitive enteropathy. Clin Chem. 1999 Dec; 45(12):2142-2149.10585346
Sblattero D, Berti I, Trevisiol C, et al. Human recombinant tissue transglutaminase ELISA: an innovative diagnostic assay for celiac disease. Am J Gastroenterol. 2000 May; 95(5):1253-1257.10811336
Schwertz E, Kahlenberg F, Sack U, et al. Serologic assay based on gliadin-related nonapeptides as a highly sensitive and specific diagnostic aid in celiac disease. Clin Chem. 2004 Dec, 50(12):2370-2375.15472035
Signorini M, Bortolotti F, Poltronieri L, Bergamini CM. Human erythrocyte transglutaminase: Purification and preliminary characterisation. Biol Chem Hoppe Seyler. 1988 Apr; 369(4):275-281.2900016
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Sugai E, Selvaggio G, Vázquez H, et al. Tissue transglutaminase antibodies in celiac disease: Assessment of a commercial kit. Am J Gastroenterol. 2000 Sep; 95(9):2318-2322.11007235
Sugai E, Vázquez H, Nachman F, et al. Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clin Gastroenterol Hepatol. 2006 Sep; 4(9):1112-1117.16860613
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LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164040 tTG/DGP Screen 63420-4 164041 tTG/DGP Screen 63420-4

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