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Bleeding With Normal aPTT/PT
Assist in the determination of a cause of bleeding in a patient with a normal aPTT and PT.
See individual tests.
Plasma (3.2% citrated), frozen
6 mL frozen citrated plasma (2 mL into each of three tubes)
3 mL frozen citrated plasma (1 mL into each of three tubes)
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482). The specimen should be frozen immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.
Freeze plasma, refrigerate serum, and maintain whole blood and buccal swab kit at room temperature or refrigerate.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|117700||Bleeding with Normal aPTT/PT||086264||Factor VIII Activity||%||3209-4|
|117700||Bleeding with Normal aPTT/PT||086298||Factor IX Activity||%||3187-2|
|117700||Bleeding with Normal aPTT/PT||086314||Factor XI Activity||%||3226-8|
|117700||Bleeding with Normal aPTT/PT||086330||Factor XIII||3241-7|
|117700||Bleeding with Normal aPTT/PT||001702||Fibrinogen Activity||mg/dL||3255-7|
|117700||Bleeding with Normal aPTT/PT||164509||vWF Activity||%||6014-5|
|117700||Bleeding with Normal aPTT/PT||086280||von Willebrand Factor (vWF) Ag||%||27816-8|
|117700||Bleeding with Normal aPTT/PT||117740||Alpha-2-Antiplasmin||%||27810-1|
|117700||Bleeding with Normal aPTT/PT||146788||Plasminogen Act Inhibitor-1||IU/mL||5975-8|