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For hours, walk-ins and appointments.Bleeding With Normal aPTT/PT
CPT:
85240; 85245; 85246; 85250; 85270; 85291; 85384; 85410; 85415
Expected Turnaround Time
4 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Plasma (3.2% citrated), frozen
Volume
6 mL frozen citrated plasma (2 mL into each of three tubes)
Minimum Volume
3 mL frozen citrated plasma (1 mL into each of three tubes)
Container
Blue-top (sodium citrate) tube
Collection
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp No. 49482). The specimen should be frozen immediately and maintained frozen until tested.
Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.
Storage Instructions
Freeze plasma, refrigerate serum, and maintain whole blood and buccal swab kit at room temperature or refrigerate.
Patient Preparation
Do not draw from an arm with a heparin lock or heparinized catheter.
Test Details
Use
Assist in the determination of a cause of bleeding in a patient with a normal aPTT and PT.
Methodology
See individual tests.
Footnotes
1. Adcock DM, Kressin DC, Marlar RA. Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol. 1997; 107(1):105-110. 8980376
2. Reneke J, Etzell J, Leslie S, et al. Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol. 1998; 109(6):754-757. 9620035
3. National Committee for Clinical Laboratory Standardization. Collection, Transport, and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays; Approved Guideline. 5th ed. Villanova, Pa: NCCLS;2008. Document H21-A5:28(5).
4. Gottfried EL, Adachi MM. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol. 1997; 107(6):681-683.9169665
5. McGlasson DL, More L, Best HA, et al. Drawing specimens for coagulation testing: Is a second tube necessary? Clin Lab Sci. 1999; 12(3):137-139. 10539100
References
Alving BM. The antiphospholipid syndrome: Clinical presentation, diagnosis, and patient management. In: Kitchens CS, Alving BM, Kessler CM, eds Consultative Hemostasis and Thrombosis. Philadelphia, Pa: WB Saunders Co;2002:181-196.
Schafer AI, Levine MN, Konkle BA, et al. Thrombotic disorders: Diagnosis and treatment. Hematology (Am Soc Hematol Educ Program). 2003; 520-539. 14633797
Triplett DA. Thrombophilia. In: McClatchey KD, ed. Clinical Laboratory Medicine. 2nd ed. Philadelphia, Pa: Lippincott Williams and Wilkins;2002:1033-1049.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 117700 | Bleeding with Normal aPTT/PT | 086264 | Factor VIII Activity | % | 3209-4 | |
| 117700 | Bleeding with Normal aPTT/PT | 086298 | Factor IX Activity | % | 3187-2 | |
| 117700 | Bleeding with Normal aPTT/PT | 086314 | Factor XI Activity | % | 3226-8 | |
| 117700 | Bleeding with Normal aPTT/PT | 086330 | Factor XIII | 3241-7 | ||
| 117700 | Bleeding with Normal aPTT/PT | 001702 | Fibrinogen Activity | mg/dL | 3255-7 | |
| 117700 | Bleeding with Normal aPTT/PT | 164509 | vWF Activity | % | 6014-5 | |
| 117700 | Bleeding with Normal aPTT/PT | 086280 | von Willebrand Factor (vWF) Ag | % | 27816-8 | |
| 117700 | Bleeding with Normal aPTT/PT | 117740 | Alpha-2-Antiplasmin | % | 27810-1 | |
| 117700 | Bleeding with Normal aPTT/PT | 146788 | Plasminogen Act Inhibitor-1 | IU/mL | 5975-8 |
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