Find Locations
For hours, walk-ins and appointments.Test Includes
Capillary electrophoresis (CE) is performed initially. If an abnormal hemoglobin is detected, appropriate testing will be performed at an additional charge. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. These tests may include high-pressure liquid chromatography (HPLC) and/or hemoglobin solubility testing.
Special Instructions
State the patient's age, ethnic background, MCV, transfusion history, and any other known hematological data on the test request form.
Expected Turnaround Time
2 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Volume
1.5 mL
Minimum Volume
0.8 mL (Note: This volume does not allow for repeat testing.)
Container
Lavender-top (EDTA) tube
Collection
To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.
Storage Instructions
Refrigerate at 2°C to 8°C.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Patient Preparation
Fasting or fluid restriction is not necessary.
Causes for Rejection
Clotted specimen; aged, improperly stored blood samples
Test Details
Use
Diagnose thalassemias and hemoglobin variants
Methodology
Capillary electrophoresis (CE); high-pressure liquid chromatography (HPLC); Hgb Solubility: Sodium hydrosulfite reduction
Reference Interval
• Hb A:
– <7 months: Not established
– 7 months to 1 year: 94.6% to 98.5%
− ≥2 years : 96.4% to 98.8%
• Hb F:
− <7 months: Not established
− 7 months to 1 year: 0.1% to 6.8%
− ≥2 years : 0.0% to 2.0%
• Hb S, D, G: 0%
• Hb C, E, O: 0%
• Hb A2:
– <7 months: Not established
– 7 months to 1 year: 1.9% to 2.8%
– ≥2 years: 1.8% to 3.2%
• Hemoglobin solubility: Negative
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 121690 | Hgb Fractionation Cascade | 43113-0 | 121692 | Hgb F | % | 32682-7 |
| 121690 | Hgb Fractionation Cascade | 43113-0 | 121693 | Hgb A | % | 20572-4 |
| 121690 | Hgb Fractionation Cascade | 43113-0 | 121694 | Hgb A2 | % | 4552-6 |
| 121690 | Hgb Fractionation Cascade | 43113-0 | 121695 | Hgb S | % | 32683-5 |
| 121690 | Hgb Fractionation Cascade | 43113-0 | 121696 | Interpretation: | 12710-0 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121697 | Hgb Solubility | 121698 | Hgb Solubility | 6864-3 | |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121697 | Hgb Solubility | 121719 | Final Interpretation: | 12710-0 | |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121712 | Hgb F | % | 42246-9 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121713 | Hgb A | % | 42244-4 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121714 | Hgb A2 | % | 42245-1 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121715 | Hgb S | % | 44923-1 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121716 | Hgb C | % | 44920-7 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121717 | Hgb E | % | 44922-3 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121718 | Hgb Variant | % | 42248-5 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121710 | Hgb Fractionation by HPLC | 121719 | Final Interpretation: | 12710-0 | |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121698 | Hgb Solubility | 6864-3 | |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121712 | Hgb F | % | 42246-9 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121713 | Hgb A | % | 42244-4 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121714 | Hgb A2 | % | 42245-1 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121715 | Hgb S | % | 44923-1 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121716 | Hgb C | % | 44920-7 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121717 | Hgb E | % | 44922-3 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121718 | Hgb Variant | % | 42248-5 |
| Reflex Table for Interpretation: | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121720 | Hgb Frac by HPLC+Solubility | 121719 | Final Interpretation: | 12710-0 | |
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