LabCorp and its Specialty Testing Group, a fully integrated portfolio of specialty and esoteric testing laboratories.
To detect and diagnose a Bordetella pertussis infection, commonly known as whooping cough
When you have persistent spasms or fits of coughing (paroxysms) that the healthcare practitioner suspects is due to pertussis (whooping cough); when you have symptoms of a cold and have been exposed to someone with pertussis
A nasopharyngeal (NP) swab or a nasal aspirate; occasionally, a blood sample drawn from a vein in your arm
Pertussis, commonly called whooping cough, is a respiratory infection caused by the bacteria Bordetella pertussis. These bacteria are highly contagious and are passed from person to person through respiratory droplets and close contact. Pertussis tests are performed to detect and diagnose a B. pertussis infection.
B. pertussis typically cause a prolonged, three-stage infection. The incubation period varies from a few days to up to three weeks.
Pertussis infections used to be very common in the United States, affecting about 200,000 people in epidemics that would occur every few years. Since the introduction of a pertussis vaccine and widespread vaccination of infants, this number has drastically decreased. (For details on the pertussis vaccine, see the Centers for Disease Control and Prevention (CDC)'s Pertussis: Summary of Vaccine Recommendations.)
However, since neither the vaccine nor the pertussis infection confers lifetime immunity, health professionals are still seeing periodic outbreaks of pertussis in young, unvaccinated infants, in adolescents, and in adults. According to the CDC, there are 10,000 to 40,000 cases reported each year. Over 48,000 cases of whooping cough were reported in 2012, the most recent peak year, and many more went unreported.
Pertussis testing is used to diagnose these infections and to help minimize their spread to others. A few different types of tests are available to detect pertussis infection:
Pertussis can be challenging to diagnose at times because the symptoms that present during the catarrhal stage are frequently indistinguishable from those of a common cold or of another respiratory illness such as bronchitis, influenza (flu), and, in children, respiratory syncytial virus (RSV).
Many adults and vaccinated people with pertussis will present with only persistent coughing and not the classic paroxysmal cough. Suspicion of pertussis infection is increased in people who have the classic "whoop," in people who have cold symptoms and have been in close contact with someone who has been diagnosed with pertussis, and when there is a known pertussis outbreak in the community. A pertussis culture and/or PCR test will usually be ordered on these people. Testing should not be performed on close contacts who do not have symptoms.
Sample collection technique is critical in pertussis testing.
For a culture or for a test for genetic material (PCR), a nasopharyngeal (NP) swab or nasal aspirate is used. The nasopharyngeal swab is collected by having the patient tip their head back and then a Dacron swab (like a long Q-tip with a small head) is gently inserted into one of the nostrils until resistance is met. It is left in place for several seconds, then rotated several times to collect cells, and withdrawn. This is not painful, but it may tickle a bit, cause the person's eyes to tear, and provoke a coughing paroxysm.
For a nasal aspirate, a syringe is used to insert a small amount of sterile saline into the nasal passage and then gentle suction is applied to collect the resulting fluid.
For antibody testing, a blood sample is obtained by inserting a needle into a vein in the arm.
Pertussis tests are used to detect and diagnose a Bordetella pertussis infection, commonly known as whooping cough, a highly contagious infection of the respiratory tract. Early diagnosis and treatment may lessen the severity of symptoms and help limit spread of the disease.
There are a few test methods that may be used to detect a pertussis infection:
Pertussis tests are ordered when someone has signs and symptoms suggestive of pertussis, and as early in the illness as possible (generally within the first two weeks for culture and the first three weeks for PCR).
Symptoms during the first stage of the infection, called the catarrhal stage, may include typical cold symptoms such as a runny nose, sneezing, mild cough, and/or a low-grade fever. After about two weeks, the paroxysmal stage begins and may include symptoms such as:
These symptoms may last for one or two weeks or persist for a couple of months. During the convalescent stage, the severity of symptoms lessens, with the frequency of coughing gradually decreasing over the next several weeks.
A positive PCR test means that it is likely that the person has pertussis. However, the PCR test may also be positive with other Bordetella species. A negative PCR test means that it is less likely that the person has pertussis but does not rule it out. If there are an insufficient number of bacteria in the sample, then they may not be detected.
A positive culture is diagnostic for a B. pertussis infection, but a negative culture does not rule it out. Culture results are dependent on proper specimen collection and transport, duration of symptoms, when the sample is collected, and prior antimicrobial therapy administered before the culture is taken.
Both culture and PCR tests are less likely to be positive as the illness progresses.
The pertussis vaccination is given to infants as a series of shots. Those children who have not completed the series of pertussis vaccinations are at a higher risk of becoming infected. Even some people who have been vaccinated may be infected by Bordetella pertussis, but they will tend to have a less severe illness.
Pertussis is treated with antibiotics, which will help to resolve the infection and help stop spread of the disease.
International travelers should be aware that many less developed countries do not have widespread vaccination for pertussis. Infants who have not completed their series of vaccinations and people who have not had a booster vaccination in many years may be at an increased risk of contracting pertussis.
Pregnant women are advised to be re-vaccinated to prevent transmission of pertussis to the newborn. Grandparents and other caretakers who will be spending time with a newborn are also advised to be re-vaccinated.
Direct fluorescent antibody (DFA) is a test method that is no longer recommended to detect pertussis infections since it is less specific and sensitive than either the pertussis culture or PCR.
A throat culture is not acceptable. During a pertussis infection, the bacteria are found in the tissues in the back of the nose, not in the throat or the front portion of the nose.
No. There is no simple, rapid diagnostic test for pertussis. It requires specialized equipment and is typically performed in laboratories. Not every laboratory performs this testing and samples may need to be sent to a public health laboratory.
Healthcare practitioners are required to report pertussis to state health departments. Outbreaks are tracked and interventions, such as vaccination and appropriate antibiotic treatment, are used to stop the outbreak.
B. parapertussis are bacteria that can infect humans in the same manner as B. pertussis, but the infection usually causes a milder respiratory illness. Culture methods and PCR tests can detect and distinguish B. parapertussis from B. pertussis, and both are commonly tested for since the signs and symptoms may be similar in people with either infection. There is no vaccine to prevent B. parapertussis infections.
Sources Used in Current Review
(August 31, 2015) Centers for Disease Control and Prevention. Pertussis. Available online at http://www.cdc.gov/pertussis/. Accessed October 2015.
(August 31, 2015) Centers for Disease Control and Prevention. Pertussis, Diagnosis Confirmation. Available online at http://www.cdc.gov/pertussis/clinical/diagnostic-testing/diagnosis-confirmation.html. Accessed October 2015.
(May 19, 2015) Centers for Disease Control and Prevention. Manual for the Surveillance of Vaccine-Preventable Diseases, Chp 10, Pertussis. Available online at http://www.cdc.gov/vaccines/pubs/surv-manual/chpt10-pertussis.html. Accessed October 2015.
(February 2015) Mayo Medical Laboratories. Hot Topic: Bordetella pertussis and Bordetella parapertussis. Available online at http://www.mayomedicallaboratories.com/articles/hot-topic/2015/02-15-pertussis/. Accessed October 2015.
(August 31, 2015) Centers for Disease Control and Prevention. Pertussis, Laboratory Information. Available online at http://www.cdc.gov/pertussis/lab.html. Accessed October 2015.
Sources Used in Previous Reviews
Wu, A. (2006). Tietz Clinical Guide to Laboratory Tests, Fourth Edition. Saunders Elsevier, St. Louis, Missouri. Pp 1536-1537.
Thomas, Clayton L., Editor (1997). Taber's Cyclopedic Medical Dictionary. F.A. Davis Company, Philadelphia, PA [18th Edition]. Pp 1454-1455.
Forbes, B. et. al. (© 2007). Bailey & Scott's Diagnostic Microbiology, Twelfth Edition: Mosby Elsevier Press, St. Louis, Missouri. Pp 435-439.
Raguckas, S. et. al. (2007 March 14). Pertussis Resurgence: Diagnosis, Treatment, Prevention, and Beyond. Medscape from Pharmacotherapy 27(1): 41-52. [On-line information]. Available online at http://www.medscape.com/viewarticle/552159. Accessed on 10/11/08.
(2008 February 1, Updated). Pertussis (Whooping Cough). Minnesota Department of Health [On-line information]. Available online at http://www.health.state.mn.us/divs/idepc/diseases/pertussis/pfacts.html. Accessed on 10/12/08.
Cornish, N. (2005 January). Identifying, testing for, and treating Bordetella pertussis. CAP Today [On-line information]. Available online at http://www.cap.org. Accessed on 10/12/08.
(2007 June 12). Pertussis. CDC Travelers' Health Yellow Book. Chapter 4, Prevention of Specific Infectious Diseases [On-line information]. Available online at http://www.cdc.gov/travel/yellowBookCh4-Pertussis.aspx. Accessed on 10/12/08.
Mayo Clinic Staff (2007 December 19). Whooping Cough. Mayoclinic.com [On-line information]. Available online at http://www.mayoclinic.com/health/whooping-cough/DS00445. Accessed on 10/12/08.
Weinberg, G. (2006 June, Revision). Pertussis. Merck Manual Home Edition [On-line information]. Available online at http://www.merck.com/mmhe/sec23/ch272/ch272g.html#sec23-ch272-ch272g-800. Accessed on 10/12/08.
(2005 Revised). Pertussis (Whooping Cough). The Merck Manual for Healthcare Professionals [On-line information]. Available online at http://www.merck.com/mmpe/sec14/ch173/ch173l.html#sec14-ch173-ch173l-774. Accessed on 10/12/08.
(2006 June). Pertussis. Guide to Surveillance, Reporting and Control. Massachusetts Department of Public Health, Bureau of Communicable Disease Control [On-line information]. PDF available for download at http://www.mass.gov/Eeohhs2/docs/dph/disease_reporting/guide/pertussis.pdf. Accessed on 10/12/08.
Carney, H. et. al. (2008 September, Updated). Bordetella pertussis. ARUP Consult [On-line information]. Available online at http://www.arupconsult.com/Topics/InfectiousDz/Bacteria/Bordetellapertussis.html#. Accessed on 10/12/08.
(2004 September). Pertussis – Laboratory Testing. Minnesota Department of Health [On-line information]. Available online at http://www.health.state.mn.us/divs/idepc/diseases/pertussis/hcp/labfacts.html. Accessed on 10/12/08/.
Gregory, D. (2006 August 1). Pertussis: A Disease Affecting All Ages. American Family Physician [On-line information]. Available online at http://www.aafp.org/afp/20060801/420.html. Accessed on 10/12/08.
Centers for Disease Control and Prevention: Guide for the Control of Pertussis Outbreaks (2000; amended in 2005, 2006). Available online at http://www.cdc.gov/vaccines/pubs/pertussis-guide/guide.htm. Accessed November 2008.
Forbes BA, Sahm DF, Weissfeld AS, Bailey & Scott's Diagnostic Microbiology 12th Edition: Mosby Elsevier, St. Louis, MO; 2007, Pp 435-438.
(August 26, 2010) Centers for Disease Control and Prevention. Pertussis. Available online at http://www.cdc.gov/pertussis/. Accessed November 2011.
(February 14, 2011) CDC. Pertussis, Diagnosis Confirmation Testing. Available online at http://www.cdc.gov/pertussis/clinical/diagnostic-testing/diagnosis-confirmation.html. Accessed November 2011.
(February 14, 2011) CDC. Best Practices for Health Care Professionals on the use of Polymerase Chain Reaction (PCR) for Diagnosing Pertussis. Available online at http://www.cdc.gov/pertussis/clinical/diagnostic-testing/diagnosis-pcr-bestpractices.html. Accessed November 2011.
(September 12, 2011) Minnesota State Department of Health. Pertussis Laboratory Testing. Available online at http://www.health.state.mn.us/divs/idepc/diseases/pertussis/hcp/labfacts.html. Accessed November 2011.
(May 26, 2009) Bocka J. Pertussis in Emergency Medicine. Medscape Reference article. Available online at http://emedicine.medscape.com/article/803186-overview. Accessed November 2011.
Frisman D, et al. Pertussis Resurgence in Toronto, Canada. BMC Public Health. 2011; 11: 694. Published online 2011 September 7. Available online at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189138/?tool=pubmed. Accessed November 2011.