Lactate Dehydrogenase (LD)

Why Get Tested?

To help detect a condition causing tissue damage, such as a blood or liver disease, and to monitor its progress; to help stage, determine prognosis, and/or response to treatment of certain cancers; to help evaluate body fluid (other than blood)

When To Get Tested?

Along with other tests, when a healthcare practitioner suspects that you have an acute or chronic condition that is causing tissue damage or cellular destruction; sometimes when you have been diagnosed with cancer; when you have symptoms of meningitis or have fluid accumulation in a specific part of your body

Sample Required?

A blood sample is obtained by inserting a needle into a vein in the arm. Sometimes a special procedure may be performed to collect body fluid from the site affected (e.g., cerebrospinal fluid, pleural fluid, peritoneal fluid, etc.).

Test Preparation Needed?

None

What is being tested?

Lactate dehydrogenase (LD or LDH) is an enzyme involved in energy production that is found in almost all of the body's cells, with the highest levels found in the cells of the heart, liver, muscles, kidneys, lungs, and in blood cells; bacteria also produce LD. This test measures the level of LD in the blood or sometimes other body fluids.

Blood LD
Only a small amount of LD is usually detectable in the fluid portion of the blood (serum or plasma). LD is released from the cells into the serum when cells are damaged or destroyed. Thus, an LD blood level is a non-specific marker for the presence of tissue damage somewhere in the body. By itself, it cannot be used to identify the underlying cause or location of the cellular damage. However, it may be used, in conjunction with other blood tests, to help evaluate for and/or monitor conditions that lead to tissue damage, such as liver or blood diseases or cancer.

Fluid LD
Sometimes when there is injury, inflammation, or infection within a specific area of the body, such as the brain, heart or lungs, fluid will accumulate or constituents of the fluid present will change. The level of LD present in the fluid may be useful in determining the cause. For example, LD is typically high in cerebrospinal fluid when an individual has bacterial meningitis. The LD test can also be used, along with other tests, to determine whether fluid accumulation, for example around the heart or lungs or in the abdominal cavity, is due to injury or inflammation (exudate) or due to an imbalance of fluid pressure inside blood vessels and the protein level in blood (transudate). The article on Body Fluid Analysis lists some fluids that may be evaluated using an LD test.

Common Questions

How is the test used?

A lactate dehydrogenase (LD or LDH) test is a non-specific test that may be used in the evaluation of a number of diseases and conditions.

An LD blood test may be used:

As a general indicator of the existence and severity of acute or chronic tissue damage
To detect and monitor progressive conditions such as anemia, including hemolytic anemia and megaloblastic anemia, or severe infections
To help stage, determine prognosis, and/or monitor treatment (i.e., chemotherapy) of cancers, such as germ cell tumors (e.g., some types of testicular cancer and ovarian cancer), lymphoma, leukemia, melanoma, and neuroblastoma

An LD test is performed on body fluids for a few different reasons:

To help evaluate cerebrospinal fluid and distinguish between bacterial or viral meningitis
To evaluate other body fluids such as pleural, peritoneal or pericardial fluid and help determine whether the accumulation of fluid is due to injury and inflammation (exudate) or due to an imbalance of pressure within blood vessels and the amount of protein in the blood (transudate). This information is helpful in guiding treatment.

The article on Body Fluid Analysis lists some fluids other than blood that may be evaluated with an LD test.

When is it ordered?

Blood test
An LD level may be ordered, along with other tests such as a comprehensive metabolic panel (CMP), when a healthcare practitioner suspects that a disease or condition is causing some degree of cellular or tissue damage. If LD is elevated, then more specific tests, such as ALT, AST or ALP, may help diagnose the condition and help determine which organs are involved. Once the acute or chronic problem is diagnosed, total LD levels may be ordered at regular intervals to monitor its progress and/or resolution.

LD levels may also occasionally be ordered when an individual has experienced muscle trauma or injury or when a person has signs and symptoms of hemolytic anemia.

LD testing may be ordered on a regular basis when an individual has been diagnosed with cancer.

Body fluid test
This test may be ordered, for example, when a person has signs and symptoms of meningitis or when someone has a buildup of fluid around the heart, lungs or in the abdomen.

What does the test result mean?

Blood test
Elevated levels of LD usually indicate some type of tissue damage. LD levels typically will rise as the cellular destruction begins, peak after some time period, and then begin to fall. LD levels are elevated in a wide variety of conditions, reflecting its widespread tissue distribution.

An elevated level of LD may be seen with:

Hemolytic anemia
Pernicious anemia (megaloblastic anemia)
Infections such as infectious mononucleosis (mono), meningitis, encephalitis, HIV
Sepsis
Intestinal, myocardial (heart) and lung (pulmonary) infarction
Acute kidney disease
Acute liver disease
Acute muscle injury
Pancreatitis
Bone fractures
Testicular cancer, lymphoma or other cancers
Severe shock
Lack of oxygen (hypoxia)

A high LD in the blood may indicate that treatment for cancer (e.g., chemotherapy) has not been successful. A high level is predictive of a poorer outlook for survival for those with cancer.

With some chronic and progressive conditions, moderately elevated LD blood levels may persist.

Low and normal levels of LD do not usually indicate a problem. Low levels are sometimes seen when someone ingests large amounts of ascorbic acid (vitamin C).

Body fluids:

Cerebrospinal fluid—a high LD indicates that meningitis is likely caused by bacteria, while a low or normal level indicates viral meningitis is more likely.
A high LD indicates that pericardial fluid, peritoneal or pleural fluid is an exudate, while a low level indicates it is transudate. Transudates are usually caused by congestive heart failure or cirrhosis. Exudates have several possible causes and usually require additional testing to determine the cause. Read more about these tests by accessing the specific fluid article listed under Related Content (below).

Is there anything else I should know?

Many things can affect LD results that are not necessarily a cause for concern. For example:

Strenuous exercise can cause temporary elevations in LD.
Hemolysis of the blood specimen can cause falsely elevated results. This may happen if the specimen is handled roughly, stored in extreme temperatures, or if the sample was difficult to collect.
If a person's platelet count is increased, serum LD can be artificially high and not reflective of the LD actually present in the circulation.

If the LD test is not specific, how can it be clinically useful?

The LD test can be useful because it can alert a healthcare practitioner that cellular injury is occurring. In a similar fashion, tests such as a C-reactive protein (CRP) warn that inflammation is occurring somewhere in the body.

Is there anything I can do to lower my LD?

No, it is an indicator of injury that will typically return to normal when the underlying condition resolves. If someone has a chronic condition with ongoing tissue damage, such as may occur with liver disease, then increased LD concentrations may persist.

What is an LD isoenzymes test?

A test for LD isoenzymes is rarely ordered and not widely available nowadays. In the past, the test was used to help diagnose and monitor heart attacks, but it has been replaced by the test for troponin. Though not a routine test, it may be used in differential diagnosis to help determine which organs are likely affected by tissue damage when the cause of an elevated total LD is not clear and cannot be determined using other specific tests.

LD exists in five different forms called isoenzymes. Although there is some overlap, each of the five LD isoenzymes tends to be concentrated in specific body tissues. In general, the isoenzyme locations tend to be:

LD-1: heart, red cells, kidneys, germ cells
LD-2: kidneys, red blood cells, lungs, heart (lesser amounts than LD-1)
LD-3: lungs and other tissues
LD-4: white blood cells, lymph nodes, muscles, liver (lesser amounts than LD-5)
LD-5: liver, skeletal muscle

Determining which isoenzyme is elevated in the blood may give clues to where tissue damage is occurring in the body and/or which organs may be affected.

Health Professionals – LOINC

LOINC Observation Identifiers Names and Codes (LOINC®) is the international standard for identifying health measurements, observations, and documents. It provides a common language to unambiguously identify things you can measure or observe that enables the exchange and aggregation of clinical results for care delivery, outcomes management, and research. Learn More.

Listed in the table below are the LOINC with links to the LOINC detail pages. Please note when you click on the hyperlinked code, you are leaving Lab Tests Online and accessing Loinc.org.

LOINC	LOINC Display Name
2528-8	LDH (CSF) [Catalytic activity/Vol]
60024-7	LDH Lactate to pyruvate reaction (CSF) [Catalytic activity/Vol]
60023-9	LDH Pyruvate to lactate reaction (CSF) [Catalytic activity/Vol]
2529-6	LDH (Body fld) [Catalytic activity/Vol]
14803-1	LDH Lactate to pyruvate reaction (Body fld) [Catalytic activity/Vol]
60017-1	LDH Pyruvate to lactate reaction (Body fld) [Catalytic activity/Vol]
33367-4	LDH (Pericard fld) [Catalytic activity/Vol]
60019-7	LDH Lactate to pyruvate reaction (Pericard fld) [Catalytic activity/Vol]
60018-9	LDH Pyruvate to lactate reaction (Pericard fld) [Catalytic activity/Vol]
2530-4	LDH (Pleur fld) [Catalytic activity/Vol]
60022-1	LDH Lactate to pyruvate reaction (Pleur fld) [Catalytic activity/Vol]
60021-3	LDH Pyruvate to lactate reaction (Pleur fld) [Catalytic activity/Vol]
2531-2	LDH (Periton fld) [Catalytic activity/Vol]
68387-0	LDH Lactate to pyruvate reaction (Periton fld) [Catalytic activity/Vol]
60020-5	LDH Pyruvate to lactate reaction (Periton fld) [Catalytic activity/Vol]
2532-0	LDH [Catalytic activity/Vol]
14804-9	LDH Lactate to pyruvate reaction [Catalytic activity/Vol]
14805-6	LDH Pyruvate to lactate reaction [Catalytic activity/Vol]

View Sources

Sources Used in Current Review

Lactate Dehydrogenase (LD), Serum. Mayo Medical Laboratories. Available online at https://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/8344. Accessed on June 13, 2018.

Lactate Dehydrogenase (LD), Body Fluid. Mayo Medical Laboratories. Available online at https://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/8022. Accessed on June 13, 2018.

Lactic Acid Dehydrogenase (Blood). University of Rochester Medical Center. Available online at https://www.urmc.rochester.edu/encyclopedia/content.aspx?contenttypeid=167&contentid=lactic_acid_dehydrogenase_blood. Accessed on 6/13/18.

Sources Used in Previous Reviews

Thomas, Clayton L., Editor (1997). Taber's Cyclopedic Medical Dictionary. F.A. Davis Company, Philadelphia, PA [18th Edition].

Pagana, Kathleen D. & Pagana, Timothy J. (2001). Mosby's Diagnostic and Laboratory Test Reference 5th Edition: Mosby, Inc., Saint Louis, MO.

Lactate Dehydrogenase. ARUP's Guide to Clinical laboratory Testing [On-line information]. Available online at http://www.aruplab.com/guides/clt/tests/clt_a32b.htm#1140532.

(2002 October 25, Updated). LDH isoenzymes. MedlinePlus Health Information [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003499.htm.

(2001 November 20, Updated). LDH. MedlinePlus Health Information [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003471.htm.

Spengler, R. (2002 May 14, Updated). What is lactate dehydrogenase (LDH)? St. Joseph's Hospital, Health Library, Medical Tests [On-line information]. Available online at http://www.sjo.org/library/healthguide/MedicalTests/topic.asp?hwid=tv6793abc.

Martin, G. (1998 March 9). The Obsolescence of Lactate Dehydrogenase Testing. American Medical Association, Archives of internal Medicine, Editor's Correspondence, Vol. 158 No. 5 [On-line information]. Available online at http://archinte.ama-assn.org/issues/v158n5/ffull/ilt0309-4.html.

Spengler, R. (2002 May 14, Updated). Cardiac Enzyme Studies. WebMD [On-line information]. Available online at http://my.webmd.com/printing/article/1675.55521.

Lactate dehydrogenase isoenzymes test. Hendrick Health System, AccessMed Health Information Library [On-line information]. Available online at http://www.hendrickhealth.org/healthy/00054240.html.

Lactate dehydrogenase test. Hendrick Health System, AccessMed Health Information Library [On-line information]. Available online at http://www.hendrickhealth.org/healthy/00054250.html.

Clarke, W. and Dufour, D. R., Editors (2006). Contemporary Practice in Clinical Chemistry, AACC Press, Washington, DC. Chapter 23 Laboratory Diagnosis of Liver Disease, Pp 269 - 279.

Wu, A. (2006). Tietz Clinical Guide to Laboratory Tests, Fourth Edition. Saunders Elsevier, St. Louis, Missouri. Pg 652.

(2007 March 13, Updated). LDH. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003471.htm. Accessed on 7/9/07.

Vorvick, L. and Zieve, D. (Updated 2009 March 14). LDH isoenzymes. MedlinePlus Medical Encyclopedia [On-line information] Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003499.htm. Accessed October 2010.

Vorvick, L. and Zieve, D. (Updated 2010 March 21). Lactate Dehydrogenase Test. MedlinePlus Medical Encyclopedia [On-line information] Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003471.htm. Accessed October 2010.

Shaffer, E. (Revised 2009 June). Testing for Hepatic and Biliary Disorders, Laboratory Tests. Merck Manual for Healthcare Professionals [On-line information] Available online at http://www.merck.com/mmpe/sec03/ch023/ch023b.html. Accessed October 2010.

(© 1995-2010). Unit Code 8344: Lactate Dehydrogenase (LD), Serum. Mayo Clinic, Mayo Medical Laboratories [On-line information] Available online at http://www.mayomedicallaboratories.com/test-catalog/Overview/8344. Accessed October 2010.

Blood Test: Lactate Dehydrogenase (LDH). KidsHealth from Nemours Foundation [On-line information] Available online at http://kidshealth.org/parent/system/medical/test_ldh.html. Accessed October 2010.

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Burtis CA, Ashwood ER, Bruns DE, eds. St. Louis: Elsevier Saunders; 2006, Pp 601-602.

National Cancer Institute. Tumor Markers. Avaialble online at http://www.cancer.gov/cancertopics/factsheet/Detection/tumor-markers. Accessed October 2013.

American Cancer Society. Specific tumor markers. Available online at http://www.cancer.org/treatment/understandingyourdiagnosis/examsandtestdescriptions/tumormarkers/tumor-markers-specific-markers. Accessed October 2013.

KidsHealth. Blood Test: Lactate Dehydrogenase (LDH). Available online at http://kidshealth.org/parent/system/medical/test_ldh.html. Accessed October 2013.

National Cancer Institute. Testicular Cancer Treatment (PDQ®). Available online at http://www.cancer.gov/cancertopics/pdq/treatment/testicular/HealthProfessional/page1#Section_562. Accessed October 2013.

Gersten, T. (Updated 2012 February 8). Lactate dehydrogenase test. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003471.htm. Accessed July 2014.

(© 1995–2014) Lactate Dehydrogenase (LD), Serum. Mayo Clinic Mayo Medical Laboratories [On-line information]. Available online at http://www.mayomedicallaboratories.com/test-catalog/Overview/8344. Accessed July 2014.

Dugdale, D. (Updated 2013 January 26). LDH isoenzymes. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003499.htm. Accessed July 2014.

Gardner, T. and Berk, B. (Updated 2013 September 3). Acute Pancreatitis. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/181364-overview. Accessed July 2014.

(Updated 2013 June 3). Acute Pericarditis. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/156951-overview. Accessed July 2014.

Dowshen, S. (Reviewed 2011 March). Blood Test: Lactate Dehydrogenase (LDH). KidsHealth from Nemours [On-line information]. Available online at http://kidshealth.org/parent/system/medical/test_ldh.html. Accessed July 2014.

Strimel, W. (Updated 2014 April 27). Pericardial Effusion. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/157325-overview. Accessed July 2014.

Rubins, J. (Updated 2013 November 18). Pleural Effusion. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/299959-overview. Accessed July 2014.

LabCorp

Find a Lab

Search Tests