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To evaluate your body's current level of iron; to help diagnose iron deficiency or iron overload
When you have low hemoglobin and hematocrit on a complete blood count (CBC); when your healthcare practitioner suspects that you may have too little iron (deficiency) or too much iron (overload) in your body
A blood sample drawn from a vein in your arm
You may be instructed to have your blood drawn in the morning and/or fast for 12 hours before the test; in this case, only water is allowed. Follow any instructions from your healthcare practitioner and/or from the laboratory performing the test.
Iron is an essential nutrient that, among other functions, is needed in small quantities to help form normal red blood cells (RBCs). It is a critical part of hemoglobin, the protein in RBCs that binds oxygen in the lungs and releases it as blood circulates to other parts of the body. The body cannot produce iron and must absorb it from the foods we eat or from supplements.
Iron tests evaluate the amount of iron in the body by measuring several substances in the blood. These tests are often ordered at the same time and the results interpreted together to help diagnose and/or monitor iron deficiency or iron overload.
Iron is normally absorbed from food in the small intestine and transported throughout the body by binding to transferrin, a protein produced by the liver. In healthy people, most of the iron transported is incorporated into the production of red blood cell hemoglobin. The remainder is stored in the tissues as ferritin or hemosiderin, with additional small amounts used to produce other proteins such as myoglobin and some enzymes.
When the level of iron is insufficient to meet the body's needs, the level of iron in the blood drops and iron stores are depleted. This may occur because:
Insufficient levels of circulating and stored iron may eventually lead to iron-deficiency anemia (decreased hemoglobin and hematocrit, smaller and paler red cells). In the early stage of iron deficiency, no physical effects are usually seen and the amount of iron stored may be significantly depleted before any signs or symptoms of iron deficiency develop. If a person is otherwise healthy and anemia develops over a long period of time, symptoms seldom appear before the hemoglobin in the blood drops below the lower limit of normal.
However, as the iron deficiency progresses, symptoms eventually begin to appear. The most common symptoms of anemia include fatigue, weakness, dizziness, headaches and pale skin. Read the article on Anemia to learn more.
Conversely, too much iron can be toxic to the body. Iron storage and ferritin levels increase when more iron is absorbed than the body needs. Absorbing too much iron over time can lead to the progressive buildup of iron compounds in organs and may eventually cause their dysfunction and failure. An example of this is hemochromatosis, a rare genetic disease in which the body absorbs and builds up too much iron, even on a normal diet. Additionally, iron overdose can occur when someone consumes more than the recommended amount of iron.
Iron tests are used to assess the amount of iron circulating in the blood, the total capacity of the blood to transport iron, and the amount of stored iron in the body. Testing may also help differentiate various causes of anemia.
Iron tests are often ordered together, and the results of each can help identify iron deficiency, iron deficiency anemia, or too much iron in the body (overload).
Iron tests may be ordered when results from a routine complete blood count (CBC) show that a person's hemoglobin and hematocrit are low and their red blood cells are smaller and paler than normal (microcytic and hypochromic), suggesting iron deficiency anemia even though other clinical symptoms may not have developed yet.
Iron tests may be ordered when a person develops signs and symptoms of anemia, such as:
Iron tests may be ordered when iron overload is suspected. Signs and symptoms of iron overload will vary from person to person and tend to worsen over time. They are due to iron accumulation in the blood and tissues. These may include:
When a child is suspected to have ingested an excessive amount of iron tablets, a serum iron test is ordered to detect and help assess the severity of the poisoning.
A summary of the changes in iron tests seen in various diseases of iron status is shown in the table below.
|Disease||Iron||TIBC/Transferrin||UIBC||% Transferrin Saturation||Ferritin|
The early stage of iron deficiency is the slow depletion of iron stores. This means there is still enough iron to make red cells, but the stores are being used up without adequate replacement. The serum iron level may be normal in this stage, but the ferritin level will be low.
As iron deficiency continues, all the stored iron is used and the body tries to compensate by producing more transferrin to increase iron transport. The serum iron level continues to decrease and transferrin and TIBC and UIBC increase. As this stage progresses, fewer and smaller red blood cells are produced, eventually resulting in iron deficiency anemia.
If the iron level is high, the TIBC, UIBC and ferritin are normal and the person has a clinical history consistent with iron overdose, then it is likely that the person has iron poisoning. Iron poisoning occurs when a large dose of iron is taken all at once or over a short period of time. Iron poisoning in children is almost always acute, occurring in children who ingest their parents' iron supplements. In some cases, acute iron poisoning can be fatal.
A person who has mutations in the HFE gene is diagnosed with hereditary hemochromatosis. However, while many people who have hemochromatosis will have no symptoms for their entire life, others will start to develop symptoms such as joint pain, abdominal pain, and weakness in their 30's or 40's. Men are affected more often than women because women lose blood during their reproductive years through menstruation.
Iron overload may also occur in people who have hemosiderosis and in those who have had repeated transfusions. This may occur with sickle cell anemia, thalassemia major, or other forms of anemia. The iron from each transfused unit of blood stays in the body, eventually causing a large buildup in the tissues. Some people with alcoholism and with chronic liver disease also develop iron overload.
Recent consumption of iron-rich foods or iron supplements can affect test results, as can recent blood transfusions.
Normal iron levels are maintained by a balance between the amount of iron taken into the body and the amount of iron lost. Normally, a small amount of iron is lost each day, so if too little iron is taken in, a deficiency will eventually develop. Unless a person has a poor diet, there is usually enough iron to prevent iron deficiency and/or iron deficiency anemia in healthy people.
In certain situations, there is an increased need for iron. Persons with chronic bleeding from the digestive tract (usually from ulcers or tumors such as colorectal cancer) or women with heavy menstrual periods will lose more iron than normal and can develop iron deficiency. Women who are pregnant or breast feeding lose iron to their baby and can develop iron deficiency if not enough extra iron is taken in. Children, especially during times of rapid growth, may need extra iron and can develop iron deficiency. Iron deficiency can also be seen in malabsorption diseases such as celiac disease.
Low serum iron can also occur in states where the body cannot mobilize and use storage iron properly. In many chronic inflammatory conditions, especially in cancers, autoimmune diseases, and with chronic inflammations or chronic infections (including AIDS), the body cannot properly use iron to make more red cells. Under these conditions, production of transferrin decreases, and serum iron is low because little iron is being absorbed from the gut and storage iron can't get mobilized, and ferritin increases.
Iron deficiency refers to a decrease in the amount of iron stored in the body, while iron deficiency anemia refers to a drop in the number of red blood cells (RBCs), hemoglobin and hematocrit caused by not having enough stored iron (there are many other causes of anemia). It typically takes several weeks after iron stores are depleted for the level of hemoglobin and production of RBCs to be affected and for anemia to develop. There usually are few symptoms early in iron deficiency, but as the condition worsens and blood levels of hemoglobin and RBCs decrease, then ongoing weakness and fatigue can eventually develop.
As your iron continues to be depleted, you may have shortness of breath and dizziness. If the anemia is severe, chest pain, headaches, and leg pains may occur. Children may develop learning (cognitive) disabilities. Besides the general symptoms of anemia, there are certain symptoms that are characteristic of iron deficiency. These include dysphagia, pica (cravings for specific substances, such as ice, corn starch, licorice, chalk, dirt, or clay), a burning sensation in the tongue or a smooth tongue, sores at the corners of the mouth, and spoon-shaped fingernails and toenails.
Several other tests can also be used to help recognize problems with iron status.
There are many different conditions that can cause anemia other than iron deficiency. Some examples include vitamin B12 and folic acid deficiencies, cancer (e.g., leukemia, lymphoma, myelodysplastic syndrome), chronic infection or inflammation, and genetic disorders such as sickle cell disease and thalassemia. However, iron deficiency is a very common cause, which is why iron tests are so frequently performed. If iron tests rule out iron deficiency, another source for the anemia must be found. Read the article on Anemia to learn more.
Heme iron is the easiest form of iron for the body to absorb. It is found in meats and eggs. Non-heme iron is found in a wide variety of plants and in iron supplements. Iron-rich sources include green leafy vegetables such as spinach, collard greens, and kale, wheat germ, whole grain breads and cereals, raisins, and molasses. If you have been diagnosed with iron deficiency anemia or you are pregnant or breast feeding, vitamin pills or tablets may be needed to provide extra iron. Ask your healthcare practitioner about the right supplement for you.
The people who typically need iron supplements are pregnant women and those with documented iron deficiency. People should not take iron supplements before talking to their healthcare practitioner as excess iron can cause chronic iron overload. An overdose of iron pills can be toxic, especially to children.
Sources Used in Current Review
Wintrobe's Clinical Hematology. 12th ed. Greer J, Foerster J, Rodgers G, Paraskevas F, Glader B, Arber D, Means R, eds. Philadelphia, PA: Lippincott Williams & Wilkins: 2009, pp 792-793, 826-827.
Harmening D, Clinical Hematology and Fundamentals of Hemostasis, Fifth Edition, F.A. Davis Company, Philadelphia, 2009, pp 122-127.
Devkota, B. (2014 January 16 Updated). Iron-Binding Capacity. Medscape Drugs and Diseases. Available online at http://emedicine.medscape.com/article/2085726-overview Accessed on June 2017.
Paruthi, S. (2015 January 14 Updated). Transferrin Saturation. Medscape Drugs and Diseases. Available online at http://emedicine.medscape.com/article/2087960-overview. Accessed on June 2017.
Harper, J. and Conrad, M. (2016 November 14 Updated). Iron Deficiency Anemia. Medscape Drugs and Diseases. Available online at http://emedicine.medscape.com/article/202333-overview Accessed on June 2017.
Devkota, B. (2014 January 16 Updated). Ferritin. Medscape Drugs and Diseases Available online at http://emedicine.medscape.com/article/2085454-overview. Accessed on June 2017.
Duchini, A. et al (2016 September 12 Updated).Hemochromatosis. Medscape Drugs and Diseases. Available online at http://emedicine.medscape.com/article/177216-overview. Accessed on June 2017.
Sources Used in Previous Reviews
Pagana, K. D. & Pagana, T. J. (© 2007). Mosby's Diagnostic and Laboratory Test Reference 8th Edition: Mosby, Inc., Saint Louis, MO. Pp 574-577.
Clarke, W. and Dufour, D. R., Editors (© 2006). Contemporary Practice in Clinical Chemistry: AACC Press, Washington, DC. Pp 407-408.
Wu, A. (© 2006). Tietz Clinical Guide to Laboratory Tests, 4th Edition: Saunders Elsevier, St. Louis, MO. Pp 634-635.
(Modified 2009 March 13). About Iron. Iron Disorders Institute [On-line information]. Available online at http://www.irondisorders.org/Disorders/about.asp. Accessed June 2009.
(Updated 2007 August 24). Dietary Supplement Fact Sheet: Iron. NIH Office of Dietary Supplements [On-line information]. Available online at http://ods.od.nih.gov/factsheets/iron.asp. Accessed June 2009.
Rathz, D. et. al. (Updated 2009 February 02). Toxicity, Iron. eMedicine [On-line information]. Available online at http://emedicine.medscape.com/article/166933-overview. Accessed June 2009.
Chen, Y. (Updated 2009 April 05). Iron Deficiency Anemia. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/000584.htm. Accessed June 2009.
Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. McPherson RA and Pincus MR, eds. Philadelphia: 2007, Pg 506-507.
(November 3, 2006) Iron Disorders Institute, Sideroblastic anemia. Available online at http://www.irondisorders.org/Disorders/Sideroblastic.asp. Accessed September 2009.
Devkota, B. (2012 October 4). Iron. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/2085704-overview#showall. Accessed April 2013.
Gersten, T. (Updated 2012 February 8). Serum iron. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003488.htm. Accessed April 2013.
Paruthi, S. (Updated 2012 November 7). Transferrin Saturation. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/2087960-overview. Accessed April 2013.
Devkota, B. (Updated October 4). Iron-Binding Capacity. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/2085726-overview. Accessed April 2013.
Spanierman, C. (Updated 2011 July 27). Iron Toxicity in Emergency Medicine. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/815213-overview. Accessed April 2013.
Boyle, J. (Updated 2012 April 12). Pediatric Iron Toxicity. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/1011689-overview. Accessed April 2013.
Gersten, T. (Updated 2012 February 8). Total iron binding capacity. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003489.htm. Accessed April 2013.
Gersten, T. (Updated 2012 February 8). Ferritin. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003490.htm. Accessed April 2013.
Pagana, K. D. & Pagana, T. J. (© 2011). Mosby's Diagnostic and Laboratory Test Reference, 10th Edition: Mosby, Inc., Saint Louis, MO. Pp 594-598.
Clarke, W., Editor (© 2011). Contemporary Practice in Clinical Chemistry, 2nd Edition: AACC Press, Washington, DC. Pp 536, 597.
Elghetany MT, Banki K. Erythrocytic disorders, in Henry's Clinical Diagnosis and Management by Laboratory Methods, 22nd ed. McPhereson RA, Pincus MR, eds. Elsevier/Saunders:Philadelphia. Chapter 32, 2011.