Patient Test Information


  • Why Get Tested?

    To determine if a person has consumed ethanol and to measure the level of ethanol in order to detect and evaluate impairment, intoxication, or overdose

    When To Get Tested?

    When someone has signs and symptoms that suggest intoxication or ethanol poisoning; when a person is suspected of violating drinking-related laws, or as part of a drug testing panel

    Sample Required?

    A breath sample is collected by blowing into a tube or balloon. A breath sample is analyzed immediately on site using a breath-analyzer. A blood sample is obtained by inserting a needle into a vein in the arm. Urine samples are collected in sterile, plastic containers. Sometimes a single urine sample is collected and sometimes two separate samples may be collected, with the first discarded and the second collected after a measured time. Rarely, saliva samples are collected from the mouth using a swab. Blood, urine, and saliva samples must be sent to a laboratory for analysis.

    Test Preparation Needed?


  • What is being tested?

    Ethanol is the intoxicating ingredient in alcoholic beverages such as beer, wine, and liquor. This test measures the amount of ethanol in the blood, urine, breath, or saliva.

    When ethanol is consumed, the gastrointestinal tract absorbs it. Ethanol is then carried throughout the body in the blood. The body eliminates small amounts of ethanol in the urine or from the lungs upon exhalation, but the liver processes (metabolizes) most ethanol.

    The liver can process about one drink an hour, with one drink being defined as the amount of ethanol in 12 ounces of beer, 5 ounces of wine, or 1.5 ounces of whisky. A person who drinks more than one drink an hour will have an increased level of ethanol in the blood. However, certain factors affect the metabolism of alcohol, especially the amount of food consumed prior to and during drinking. The rate of metabolism can also vary with age, gender, weight, concurrent medications, and genetic status.

    Drinking alcoholic beverages faster than the liver can process can result in an elevated level of ethanol in the blood. This leads to signs and symptoms of intoxication such as bloodshot eyes, flushed face, slurred speech, slow response to questions or comments, impaired judgment, decreased motor skills, drowsiness or falling asleep, and/or vomiting.

    With a very high blood ethanol, more serious signs and symptoms of toxicity may appear such as confusion, stupor, staggering, irregular or slow breathing, loss of consciousness, seizures, and low body temperature (hypothermia). A very high blood ethanol can be fatal.

    Long-term, excessive alcohol use can lead to the development of several medical problems such as liver disease, cardiovascular problems, depression and anxiety. (Read more about this in the article on Alcoholism.)

  • How is it used?

    The ethanol test, commonly known as alcohol test, is used for both medical and legal purposes. Samples and results for each use are usually collected and tested separately.

    Medical: medical testing is used to determine the level of ethanol in the blood in order to effectively treat the intoxicated person's symptoms. Blood is the most common sample used for medical alcohol tests. The tests may be ordered for a person who presents to the emergency room with signs and symptoms suggesting ethanol toxicity.

    Metabolites of ethanol, including ethyl glucuronide or ethyl sufate, can also be examined. Additional tests, such as a complete blood count (CBC), glucose, and electrolyte measurement, are often ordered at the same time because there are a variety of other conditions that can cause symptoms similar to ethanol intoxication.

    Overdose testing, drugs of abuse testing, and testing for the presence of other toxic alcohols such as methanol and isopropyl alcohol may also be performed if a person is suspected of ingesting or using other substances.

    Legal (Forensic): legal testing is used to identify the presence of alcohol and to evaluate its presence in the context of a variety of different laws. Legal testing must have a strict chain-of-custody. Testing may be ordered to determine, for example, whether:

    • A driver has a blood alcohol concentration (BAC) that is over the legal limit
    • An under-age minor has been drinking
    • Someone on parole has abstained from alcohol
    • Alcohol consumption has contributed to an accident

    Post-mortem ethanol testing may be done to determine whether alcohol contributed to a person's death.

    Legal ethanol testing may also be performed randomly as part of an employer's drug testing program or it may be performed "with cause" as part of an investigation after an on-the-job accident has occurred. It may also be done as part of an application for life insurance. These uses are considered legal alcohol tests because they require chain-of-custody documents.

    Samples tested for legal purposes may include blood, breath, urine, and/or saliva. Breath testing is the most common test performed on drivers. It uses a conversion factor to estimate the amount of alcohol in the blood.

    Blood ethanol testing may be ordered to confirm or refute findings and/or ordered as an alternative to breath testing. Urine testing may also be performed as an alternative. Usually, a person collects and discards a urine sample and then collects a second sample 20 to 30 minutes later. The amount of alcohol in the first sample will be variable because it is unknown how long the urine has been in the bladder. The second sample will reflect a timed sample and a conversion factor can then be used to estimate blood alcohol. A random urine sample is sometimes ordered to monitor people for the presence of alcohol. Saliva alcohol testing is not as widely used but may be used as an alternate screening test.

    When is it ordered?

    Medical ethanol testing is ordered when a person has signs and symptoms that suggest intoxication such as:

    • Bloodshot, glassy, or watery eyes
    • Flushed face
    • Droopy eyelids
    • Blank stare or dazed look
    • Twitching or body tremors
    • Thick, slurred, or loud speech
    • Rambling train of thought
    • Unusually fast or slow talking
    • Slow response to questions or comments
    • Repetitive or irrational statements
    • Lethargy, drowsiness or falling asleep
    • Vomiting

    With higher blood ethanol levels, more serious signs and symptoms may appear. These may include:

    • Confusion, stupor
    • Staggering
    • Irregular (long gaps between breaths) or slow breathing (less than eight breaths a minute)
    • Unconsciousness
    • Seizures
    • Blue-tinged skin or pale skin
    • Low body temperature (hypothermia)

    Medical testing may also be done on a regular basis to ensure that a person who is being treated is not continuing to abuse ethanol.

    Legal ethanol testing may be ordered when there is suspicion that a person has not followed a drinking-related law and whenever there has been an accident or unexpected death. Employment alcohol testing may be performed randomly and when the employer suspects that an employee has alcohol in his or her system while on the job. Insurance testing is primarily performed when someone is applying for a policy.

    What does the test result mean?

    For medical testing, the detection of ethanol in a sample indicates that a person has likely been drinking, and the concentration present can give an indication of the degree of intoxication. Symptoms and complications may vary significantly from person to person. The individual's general health, age, and other medications or drugs that he or she is taking can all affect an individual's symptoms. The ability to clear the alcohol out of the body also depends on the health and function of the person's liver.

    For legal testing, results obtained are compared to legal allowable limits.

    The table below lists some possible interpretations of blood ethanol results:

    Blood Ethanol Result Interpretation
    Equal to or above 80 mg/dL (0.08%) Legal intoxication in all states
    80 to 400 mg/dL (0.08% to 0.40%) Increasing impairment and depression of central nervous system likely
    Above 400 mg/dL (>0.40%) Loss of consciousness likely; potentially fatal

    Someone who chronically consumes significant amounts of ethanol can develop a tolerance to it and may show fewer signs and symptoms and visible impairment at a higher blood alcohol level than someone who does not consume ethanol as often.

    Is there anything else I should know?

    Different ethanol sample results are not interchangeable. Breath samples are considered to be good estimates of blood alcohol concentrations in most people but can be affected by alcohol consumption within the last few minutes, ketones, released into the breath by some diabetics and dieters, and by other substances that contain alcohol, such as mouthwash and cough syrup.

    Urine concentrations lag behind blood concentrations. Peak urine alcohol levels are reached 45 to 60 minutes after alcohol ingestion, at which time levels are typically about 1.3 times greater than the corresponding blood alcohol concentration, but this can be affected by various factors. Sometimes two separate samples may be collected with the first discarded and the second collected after a measured time (20-30 minutes) and tested for ethanol. This practice provides better correlation between urine and blood ethanol levels.

    Urine samples that contain both glucose and bacteria or yeast (such as may be seen in some diabetics) should not be left at room temperature for extended periods of time as there is the potential for the microorganisms present to ferment the glucose in the sample and produce ethanol. This can also be seen in post-mortem samples. Occasionally, two serotonin metabolites, 5-HIAA (5-hydroxyindoleacetic acid) and 5-HTOL (5-hydroxytryptphol), may be tested to evaluate this phenomenon and confirm ethyl alcohol ingestion. An increased ratio of 5-HTOL/5-HIAA can be indicative of alcohol consumption.

    Children frequently develop low blood sugar (hypoglycemia) along with ethanol poisoning, so healthcare practitioners may order blood glucose tests along with ethanol tests if they suspect ethanol toxicity in youngsters.

    Can medical providers release medical ethanol test results for legal purposes?

    The Health Insurance Portability and Accountability Act (HIPAA) Privacy Rule allows healthcare providers in certain circumstances to disclose necessary medical information about a patient to law enforcement if they believe the patient presents a serious danger to himself or other people. State rules regarding release of medical test results for legal purposes vary.

    Does everyone metabolize alcohol at the same rate?

    A general rule of thumb is one drink equivalent an hour, but there is individual variation due to differences in factors such as race, sex, body weight, use of legal or illegal drugs that interact with alcohol, and food consumption.

    Is there anything I can do to get rid of the alcohol in my system faster?

    No, alcohol must be metabolized and eliminated by the liver and the rate at which this happens is regulated by the liver. Food will slow the absorption of ethanol and coffee may make someone who is intoxicated feel more alert, but neither will speed up the process.

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