Patient Test Information

Drug Abuse Testing

  • Why Get Tested?

    To screen for drug abuse, to monitor someone with a substance abuse problem, to monitor compliance with prescribed medications, or to detect and evaluate drug intoxication or overdose

    When To Get Tested?

    Sometimes required prior to the start of a new job or insurance policy; randomly for workplace drug testing or athletic drug testing programs; as mandated when court-ordered; as indicated when ordered by a health practitioner to monitor a known or suspected substance abuse patient; sometimes when you are pregnant, will be receiving an organ transplant, when you are prescribed pain medication, or when you have symptoms suggesting drug intoxication or overdose

    Sample Required?

    A random urine sample; sometimes a blood sample drawn from a vein in your arm; hair, saliva, or sweat

    Test Preparation Needed?

    Some prescription and over-the-counter drugs may give a positive screening result; prior to testing, indicate any medications that you have taken and/or for which you have prescriptions.

  • What is being tested?

    Drugs of abuse testing is the detection of one or more illegal and/or prescribed substances in the urine, blood, saliva, hair, or sweat. Testing detects substances not normally found in the body, with the exception of some hormones and steroids measured as part of sports testing.

    Drug abuse testing usually involves an initial screening test followed by a second test that identifies and/or confirms the presence of a drug or drugs. Most laboratories use commercially available tests that have been developed and optimized to screen urine for the "major drugs of abuse."

    For most drugs of abuse testing, laboratories compare results of initial screening with a predetermined cut-off. Anything below that cut-off is considered negative; anything above is considered a positive screening result. In addition, labs might perform testing for masking agents (adulterants). These may either interfere with testing or dilute a urine sample.

    Among drugs of abuse, each class of drug may contain a variety of chemically similar substances. Legal substances that are chemically similar to illegal ones can produce a positive screening result. Positive screening tests are considered presumptive. Therefore, screening tests that are positive for one or more classes of drugs are frequently confirmed with a secondary test that identifies the exact substance present using a very sensitive and specific method, such as gas chromatography/mass spectrometry (GC/MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS).

    Some of the most commonly screened drug classes are listed in the table below.

    Drug class screened Examples of specific drugs identified during confirmation
    Amphetamines Methamphetamine, amphetamine
    Barbiturates Phenobarbital, secobarbital, pentobarbital, butalbital, amobarbital
    Benzodiazepines Diazepam, lorazepam, oxazepam, temazepam, alprazolam
    Cannibinoids Marijuana
    Cocaine Cocaine and/or its metabolite (benzoylecognine)
    Methadone Methadone, methadone metabolite (EDDP)
    Opiates Codeine, morphine, metabolite of heroin, oxycodone, oxymorphone, hydrocodone, hydromorphone
    Phencyclidine PCP

    See a more comprehensive listing of drug classes and drugs of abuse in the table below.

    Substances that are not similar to the defined classes can produce negative results even though they are present. Some drugs may be difficult to detect with the standardized assays, either because the test is not set up to detect the drug, such as methylenedioxy-methamphetamine (MDMA, also known as Ecstasy or Molly), fentanyl, methadone, oxycodone (Oxycontin), meperedine, or buprenorphine, or because the drug does not remain in the body long enough to be detected, such as gamma-hydroxybutyrate (GHB).

    For sports testing of hormones and steroids, each test performed is usually specific for a single substance and may be quantitative. Athletes, especially those at the national and international levels, are tested for illegal drugs and are additionally prohibited from using a long list of substances called "performance enhancers."

    Groups of drug tests are typically ordered for medical or legal reasons, as part of a "drug-free workplace," as part of a sports testing program, or to determine compliance with prescribed (pain) medications. People who use these substances ingest, inhale, smoke, or inject them into their bodies. How much of these drugs the body absorbs and their effects depend on the substances, how they interact, their purity and strength, their quantity, timing and method of intake, and an individual's ability to metabolize and eliminate them from the body.

    Some drugs can interfere with the action or metabolism of other medications, or have additive effects, as in the case of taking two drugs that both depress the central nervous system (CNS). Drugs may also have competing effects, as can happen when one drug that depresses the CNS and another that stimulates it are taken.

    How is the sample collected for testing?

    Urine is the most frequently tested sample in drug abuse screening. Other body samples, such as hair, saliva, sweat, and blood, also may be used but not interchangeably with urine.

    Urine and saliva are collected in clean containers. A blood sample is obtained by inserting a needle into a vein in the arm. Hair is cut close to the scalp to collect a sample. A sweat sample is typically collected by applying a patch to the skin for a specified period of time.

    Is any test preparation needed to ensure the quality of the sample?

    Certain prescription and over-the-counter drugs may give a positive screening result. Examples of false-positive screening results: Vicks nasal spray can test positive for amphetamines; poppy seeds can produce a false-positive for opiates. Prior to testing, you should declare any medications that you have taken and/or for which you have prescriptions so that your results can be interpreted correctly.

  • How is the test used?

    Drugs of abuse testing is used to screen for and confirm the presence of several drugs in a person's sample, such as urine, blood or hair. Drug testing is used so that a person may receive appropriate medical treatment or be screened for or monitored for illegal drug use.

    Some of the most commonly screened drugs include:

    • Amphetamine and methamphetamine
    • Barbiturates such as phenobarbital, secobarbital and pentobarbital
    • Benzodiazepines such as diazepam, lorazepam and oxazepam
    • Marijuana
    • Cocaine
    • Methadone
    • Opiates, such as heroin, codeine and morphine
    • Phencyclidine (PCP)

    See a more comprehensive listing of drug classes and drugs of abuse in the table below.

    Drugs of abuse testing may be used for:

    • Medical screening
    • Legal or forensic information
    • Employment drug testing
    • Sports/athletics testing
    • Monitoring pain medication use

    Medical Screening 
    Medical screening for drugs of abuse is primarily focused on determining what drugs or combinations of drugs a person may have taken so that the person can receive proper treatment. A drug's overall effect on a particular person depends on the response of the person's body to the substance, on the quantity and combination taken, and when it was taken. For instance, MDMA (also known as Ectasy or Molly) is initially a stimulant with associated psychedelic effects but later causes central nervous system (CNS) depression as it is metabolized and cleared from the body. In many cases, drugs have been combined and/or taken with ethanol (alcohol). If someone drinks ethanol during this time period, the person will have two CNS depressants in their system, a potentially dangerous combination.

    A health practitioner may order drug testing for medical reasons if an individual:

    • Has acute health problems that may be drug-related or shows signs of intoxication, such as unconsciousness, nausea, delirium, panic, paranoia, increased temperature, chest pain, respiratory failure, seizures, and/or headaches
    • Has injuries from an accident that an emergency room physician suspects are due to drugs and/or alcohol
    • Is suspected of drug use or is known to use legal and/or illegal drugs
    • Is a candidate to receive an organ transplant
    • Is pregnant, especially if she is thought to be at risk for drug abuse or has a newborn baby exhibiting certain characteristic behaviors
    • Is on pain medication, to ensure that the person is taking the medication as prescribed

    For a more in-depth discussion of this, see the article Emergency and Overdose Drug Testing.

    Legal or Forensic Testing 
    Drug testing for legal purposes primarily aims to detect illegal or banned drug use in a variety of situations. Sample collection procedures for this type of testing are strictly controlled and documented to maintain a legal "chain-of-custody." The donor provides a sample that is closed and secured with a tamperproof seal in his or her presence. Specific chain-of-custody paperwork then accompanies the sample throughout the testing process. Each person who handles and/or tests the sample provides their signature and the reason for the sample transfer. This creates a permanent record of each step of the process.

    Examples of legal drug abuse screening include:

    • Court-mandated testing usually involving random monitoring of someone who has been convicted of illegal drug use or to rule out drug use by parents involved in custody cases.
    • Extended monitoring of a parent with a history of drug abuse, ordered by government child protective services to ensure that the person has not resumed use of substances.
    • Testing by law enforcement when alcohol and/or drugs are thought to be involved in an accident
    • Forensic toxicology testing using a variety of body fluids and tissues that may be tested during a crime investigation; the goal may be to determine whether drugs were a contributing factor to an accident or crime, such as DUI (driving under the influence) or rape. Testing may also determine whether someone died of a drug overdose or drug-related condition.
    • Applicants for life insurance may undergo drug screening, possibly including tests for cocaine and nicotine, even though tobacco is a legal substance.
    • Schools may have programs that incorporate random drug testing. Such programs may look for use of illegal drugs or performance-enhancing substances among athletes.

    For additional information on this type of testing, see the article on The World of Forensic Laboratory Testing.

    Employment Drug Testing 
    Employment drug testing is a specific type of forensic testing (see above). It may be done prior to employment, on a random basis, following an accident, or if the employer has a reasonable suspicion that an employee is using illegal drugs. Employment drug testing is commonplace. It is required in some industries, such as those that involve the U.S. Department of Transportation, the military and federal employees, and is an accepted practice in many other industries.

    As with legal or forensic drug testing, the sample collection and testing procedures for employment drug testing are often strictly controlled and documented to maintain a legal "chain-of-custody."

    Sports/Athletics Screening 
    While conventional drug testing is performed on competitive athletes, the primary focus is on doping, the use of drugs and/or supplements intended to promote muscle growth and/or to improve strength and endurance. On a local level, sports testing may be limited, but on a national and international level, it has become highly organized.

    The World Anti-Doping Agency (WADA), U.S. Anti-Doping Agency (USADA), and the International Association of Athletics Federations (IAAF) work together to monitor athletes' drug use on a national, international, and Olympic level. WADA has a written code that establishes uniform drug testing rules and sanctions for all sports and countries and that includes a substantial list of prohibited substances. Athletes are responsible for any banned substances found in their body during testing.

    Most compounds are prohibited in any quantity, while others, such as caffeine, are only prohibited when they are present in large amounts. Some of the substances, such as anabolic steroids (testosterone) and peptide hormones such as erythropoietin, growth hormone, and insulin-like growth factor-1 are banned but are difficult to measure because the body also produces them naturally. Testing methods must be able to distinguish between endogenous (that produced by the athlete's body) and supplemented compounds.

    Screening programs randomly perform out-of-competition drug tests on athletes during the training season to look for anabolic steroids, such as testosterone, that promote increased muscle growth. During competitions, testing is frequently done both randomly and on all winners.

    Testing includes categories such as stimulants, narcotics, anabolic agents, and peptide hormones. WADA bans use of beta blockers in archery, golf, shooting, freestyle skiing, and snowboarding because they decrease blood pressure and heart rate and have a calming effect that can enhance balance and steadiness of the hands.

    While professional sports organizations, such as the NFL (National Football League), NHL (National Hockey League), and NBA (National Basketball Association), are not covered by the WADA code, they have programs in place to test their athletes for panels of drugs that combine aspects of sports and employment testing. Those professional athletes who also take part in the Olympics, however, are subject to Olympic regulations.

    Monitoring Pain Medication Use
    Drug screening in urine is the method of choice for monitoring adherence to prescription pain medications in patients treated for chronic pain. Urine drug screening provides tools for tracking patient compliance and exposing possible drug misuse and abuse. A health practitioner may periodically order drug testing for patients treated for chronic pain for two purposes: 1) ensuring compliance by patients who are using the prescribed pain medications, and 2) monitoring the use of non-prescribed or illegal substances. Elements of a urine drug screen may include specific gravity, temperature at the time of sample collection, pH, creatinine concentration, and confirmatory testing.

    Compliance monitoring of pain medications is crucial for eliminating or significantly curtailing abuse (e.g., addiction, diversion) of controlled prescription drugs and at the same time assuring the appropriate treatment for those patients who can be helped by these medications. Urine drug screening is considered one of the mainstays of adherence monitoring in conjunction with prescription monitoring programs and other screening tools.

    When is it ordered?

    Drug testing is performed whenever a health practitioner, employer, legal entity, or athletic organization needs to determine whether a person has illegal or banned substances in his or her body or is compliant with prescribed medications. It may be ordered prior to the start of some new jobs and insurance policies and at random to satisfy workplace, court-ordered, adherence monitoring, or athletic drug testing program requirements.

    Drugs of abuse testing may also be ordered by a health practitioner to monitor a known or suspected substance abuse patient or whenever a person has signs and symptoms that suggest drug use. Depending on the drug or drugs involved, these may include, for example:

    • Dilated or small pupils
    • Drowsiness
    • Slow or slurred speech
    • Agitation
    • Nausea
    • Difficulty breathing
    • Delirium
    • Seizures
    • Changes in blood pressure or heart rhythm

    What does the test result mean?

    A positive initial drug screening means that the person tested has a substance in his or her body that falls into one of the drug classes and is above the established cutoff level. If secondary testing confirms a positive result, it means that the person has indeed taken this drug.

    In some cases, this result indicates a window of time in which the person took the substance and its approximate quantity, but in most circumstances, that information is not necessary. Interpretation of when and how much drug was consumed can be challenging because the concentration of many drugs varies, as do people's rates of metabolism.

    If testing reveals no drugs, or drugs in amounts below the established cutoffs, then the results are usually reported as "not detected" or "none detected."

    A negative result does not necessarily mean that the person did not take a drug at some point. The drug may be present but below the established cutoff, the drug may have been already metabolized and eliminated from the body, or the test method does not detect the particular drug present in the sample.

    Tests of different body samples reveal drug use within varying windows of time. For example:

    • Urine testing shows drug use over the last 2 or 3 days for amphetamines, cocaine, and opiates. Marijuana and its metabolites, cannabinoids, may be detectable for several weeks. Methadone, barbiturates and benzodiazepines can be detected for about a week.
    • Hair samples, which test the root end of the hair, reflect drug use within the last 2 to 3 months but not in the amount of time it takes for the hair to grow, the most recent 2 to 3 weeks.
    • Saliva detects which drugs have been used in the last 24 hours.
    • Samples of sweat may be collected on an absorbent patch worn for several days to weeks and therefore can indicate drug use at any point during that extended period of time.

    Different types of samples are often used for specific purposes. For instance, hair samples may be used as an alternative to urine testing for employment or accident drug testing. Sweat testing may be used as a court-ordered monitoring tool in those who have been convicted of drug use, while saliva is often used by the insurance industry to test insurance applicants for drug use. Blood is most frequently used for alcohol testing.

    Interpretation of sports testing results for hormones and steroids should be done only by laboratory professionals who are familiar with particular test methods. A negative result indicates that there is a "normal" amount of the substance present in the body. Positive results reflect the presence of the substance above and beyond what is normally produced by the athlete's body. These results can be complicated by the fact that each person will have his or her own normal baseline concentration and will produce varying amounts of hormones and steroids, depending upon the circumstances.

    Why doesn't a drug screen measure all drugs?

    Drug tests are set up for their specific uses, typically workplace and legal testing. Some laboratories may offer more than one drug screen panel or a health practitioner, or other authorized entity, may select tests individually.

    Why might a healthcare provider only test for a single drug?

    If a health practitioner strongly suspects that a person has taken a specific drug or is monitoring the person for previous abuse of that substance, then the practitioner may order only that drug test. Another reason for ordering a single test is if the suspect drug is not part of an offered drug screen.

    Why would someone be tested for something that is legal?

    A substance can be legal but may be banned in certain circumstances. For instance, an insurance company may test an insurance applicant for nicotine to verify the person's assertion that he or she is not currently using tobacco. In the case of prescription medications, the high addictive potential for certain medications makes drug testing important. In the case of some athletes, many of the prohibited substances are legal but have the potential to affect the athlete's performance. Athletes must exercise caution in their choices of medications as they are ultimately responsible for any substances detected in their bodies.

    Is there anything else I should know?

    Symptoms associated with drug abuse and drug overdose will vary from person to person, from time to time, and do not necessarily reflect drug concentrations in the body.

    Ethanol may be measured in both the blood and the breath. This is the basis for the breathalyzer test used by law enforcement.

    For some types of testing, such as workplace testing of federal employees, there are many regulations that cover the test from collection through interpretation and reporting of results. It is important for the ordering physician, law enforcement representative, forensic professional, government entity, insurance agent, employer, and sports organization as well as for the person being tested to understand what exactly is included in the testing, how it is done, and how the results may or may not be interpreted. This process is not nearly as simple or straightforward as collecting a sample and requesting "drug testing."

    Prescription drug monitoring programs incorporate urine drug testing as part of the process of monitoring adherence to prescription pain medications.

  • View Comprehensive Table of Drugs that May Be Tested

    Examples of Drugs of Abuse

    Stimulants Amphetamine Dexedrine, Adderall, Benzedrine Speed, Bennies, Uppers, Black Beauties, Crosses, Hearts, LA Turnaround, Speed, Truck Drivers
    Cocaine Cocaine hydrochloride topical solution (anesthetic rarely used in medical procedures) Crack, Coke, Candy, Blow, C, Flake, Rock
    Methamphetamine Desoxyn, Desoxyn, Gradumet Crank, Chalk, Crystal, Fire, Glass, Go Fast, Ice, Meth, Speed
    Methylphenidate Ritalin, Concerta JIF, MPH, R-ball, Skippy, The Smart Drug, Vitamin R
    Phenylpropanolamine Propagest BT 72s
    Methylenedioxymethamphetamine MDMA Ecstasy, Molly, Adam, Clarity, Eve, Lover's Speed, Peace
    Depressants/Sedatives Barbiturates Phenobarbital (Luminal), pentobarbital (Nembutal), secobarbital Barbs, Phennies, Red Birds, Reds, Tooies, Yellow Jackets, Yellows
    Benzodiazepines alprazolam (Xanax®), chlorodiazepoxide (Limbitrol®), diazepam (Valium®), lorazepam (Ativan®), triazolam (Halicon®) Pumpkin Seeds, Downers, Candy, Tranks, Sleeping Pills
    Ethanol Alcohol Beer, wine, distilled alcohol
    Ethchlorvinyl Placidyl Pickles, Green Jeans
    Flunitrazepam Rohypnol Circles, Date Rape Drug, Forget Pill, Forget-Me Pill, La Rocha, Lunch Money, Mexican Valium, Mind Eraser, Pingus, R2, Reynolds, Rib, Roach, Roach 2, Roaches, Roachies, Roapies, Rochas Dos, Roofies, Rope, Rophies, Row-Shay, Ruffies, Trip-and-Fall, Wolfies
    Gamma-hydroxybutyrate GHB G, Georgia Home Boy, Goop, Grievous Bodily Harm, Liquid Ecstasy, Liquid X, Soap, Scoop
    Glutethimide Doriden CB
    Meprobamate Miltown Bams
    Methaqualone Quaaludes Soapers, 714, Ludes
    Methyprylon Noludar Roach 19, Easter Bunny
    Sleeping aids eszopiclone (Lunesta®), zaleplon (Sonata®), zolpidem (Ambien®) Forget-me Pill, Mexican Valium, R2, Roche, Roofies, Roofinol, Rope, Rophies
    Dissociative Drugs Dextromethorphan or DMX Over-the-counter cough and cold medications (many brand names include "DM") Robotripping, Robo, Triple C
    Ketamine Ketalar Cat Valiums, Special K, Vitamin K
    Phencyclidine PCP Angel Dust, Boat, Hog, Love Boat, Peace Pill
    Salvia divinorum Salvia Maria Pastora, Sage of the Seers, Diviner's Sage, Salvia, Sally-D, Magic Mint
    Opiates/Synthetic Opioids Oxycodone Percodan, Percocet, Oxycontin O.C., Oxycet, Oxycotton, Oxy, Hillbilly Heroin, Percs
    Oxymorphone Opana Biscuits, Blue Heaven, Blues, Mrs. O, O Bomb, Octagons, Stop Signs
    Fentanyl Duragesic, Actiq, Sublimaze Apache, China Girl, China White, Dance Fever, Friend, Goodfella, Jackpot, Murder 8, Tango and Cash, TNT
    Heroin   Brown sugar, China White, Dope, H, Horse, Junk, Skag, Skunk, Smack, White Horse
    Hydromorphone Dilaudid D, Dillies, Footballs, Juice, Smack
    Methadone Diskets, Dolophine, Methadose, Methadose Sugar-Free Orange Barrel, Dolphin, Amidone, Fizzies
    Codeine Tylenol 3 Captain Cody, Cody, Lean, Schoolboy, Sizzurp, Purple Drank With glutethimide: Doors & Fours, Loads, Pancakes and Syrup
    Hydrocodone Vicodin, Lortab, Lorcet Vike, Vic, Watson-387
    Morphine MS Contin®, Avinza, Duxamorph, Roxanol Morph, Miss Emma, M, White Stuff
    Meperedine Demerol Demmies, Pain Killer
    Opium   Dust
    Pentazocine Talwin T's
    Propoxyphene Darvon Lilly
    Psychedelics (Hallucinogens) Lysergic acid diethylamide LSD Acid, Blotter, Blue Heaven, Cubes, Microdot, Yellow Sunshine
    Mescaline Peyote Aztec, Blue Cap, Buttons, Cactus, Mesc
    Psilocybin   Little Smoke, Magic Mushrooms, Purple Passion, Shrooms
    Cannabinoids Dronabinol (THC) Marinol, Sativex  
    Hash Oil   Honey Oil, Shatter, Wax, Ear Wax
    Hashish   Hash
    Marijuana   Blunt, Bud, Dope, Ganja, Grass, Green, Herb, Joint, Mary Jane, Pot, Reefer, Sinsemilla, Skunk, Smoke, Trees, Weed; Hashish: Boom, Gangster, Hash, Hemp
    Synthetic Cannibinoids Synthetic Marijuana Spice K2, Spice, Black Mamba, Bliss, Bombay Blue, Fake Weed, Fire, Genie, Moon Rocks, Skunk, Smacked, Yucatan, Zohai
    Synthetic Cathinones Bath Salts   Bloom, Cloud Nine, Cosmic Blast, Flakka, Ivory Wave, Lunar Wave, Scarface, Vanilla Sky, White Lightning
    Tobacco Nicotine Cigarettes, cigars, chewing tobacco Snuff, chew
    Inhalants   Glue, paint thinner, gasoline, hair spray, spray paint Poppers, Snappers, Whippets, Laughing Gas
    Sports Drugs/Doping Anabolic Agents Nandrolone (Oxandrin®), oxandrolone (Anadrol®), oxymetholone (Winstrol®), stanozolol (Durabolin®), testosterone cypionate (Depo-testosterone®) Stackers, Arnolds, Gym Candy, Pumpers, Roids, Weight Trainers, Gear, Juice
    Beta Blockers Acebutolol (Sectral), Atenolol (Apo-atenolol), Betaxolol (Kerlone), Bisoprolol (Zebta), Carteolol (Cartrol), Esmolol (Brevibloc), Labetalol (Normodyne), Metoprolol (Toprol), Nadolol (Corgard), Sotalol (Betapace), Oxprenolol (Trasicor), Penbutolol (Levatol), Pindolol (Novo-pindol), Propranolol (Inderal), Timolol (Apo-timol)  
    Erythropoietin, EPO Several, including Anfoe, Celpoeitin, Ceriton, Cres[, Epofer, Epodit, Epomine, Eporise, Eposis, Epox, Eptre, Erypo, Hemas, Neo Recormon, RPO, Vintor, Wepox, Zyrop  
    Peptide Hormones Growth Hormone  
    Insulin-like growth factor-1 IGF-1  
    Human chorionic gonadotropin hCG, Novarel, Ovidrel, Pregnyl  
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