For the safety of our patients and employees in Texas, Oklahoma, Missouri and Arkansas, the current winter weather may affect patient service center hours. Please review the list of affected locations for further information.
Synonyms
- FLT3 D835 mutation
- FLT3 Internal Tandem Duplication and Tyrosine Kinase Domain mutation
- FLT3 ITD and D835 mutations
- FLT3 ITD and TKD mutations
- FLT3 ITD mutation
- FLT3 TKD mutation
Special Instructions
Please direct any questions regarding this test to customer service at 800-345-4363.
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
|
3 - 5 days 3 - 5 days |
|
3 - 5 days 3 - 5 days |
Related Information
|
Specimen Requirements
Specimen
Whole blood, bone marrow or cell pellet
Volume
3-5 mL whole blood or 1-2 mL bone marrow
Minimum Volume
3 mL whole blood or 1 mL bone marrow
Container
Lavender-top (EDTA) tube, green-top (sodium heparin) tube, tan-top (K2-EDTA) tube or pink-top (K2-EDTA) tube
|
Lavender-top (EDTA) tube |
|
Lavender-top (EDTA) tube, green-top (sodium heparin) tube, tan-top (K2-EDTA) tube or pink-top (K2-EDTA) tube |
Collection
Ship specimen at room temperature. Specimen should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the request form.
Storage Instructions
Refrigerate. If specimen is to be stored prior to shipment, store at 2°C to 8°C.
Causes for Rejection
Specimen does not meet collection criteria; frozen whole blood, marrow, or cell pellet; leaking tube; clotted blood or marrow; grossly hemolyzed specimen or otherwise visibly degraded; contamination by another specimen; specimens containing suspicious foreign material
Test Details
Use
Mutations in the FLT3 gene are common mutations in acute myeloid leukemia (AML). This assay detects internal tandem duplication (ITD) mutations and mutations in the tyrosine kinase domain (TKD) of FLT3. Presence of these mutations in AML provide prognostic information and can aid in the determination of therapeutic regimen.
Limitations
This PCR assay is capable of detecting a mutant cell population with a sensitivity of 5 mutant cells per 100.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
|
This PCR assay is capable of detecting a mutant cell population with a sensitivity of 5 mutant cells per 100. This test was developed and its performance characteristics determined by Lab |
|
This PCR assay is capable of detecting a mutant cell population with a sensitivity of 5 mutant cells per 100. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration. |
Methodology
Polymerase chain reaction (PCR); restriction enzyme digest; capillary electrophoresis
Additional Information
FLT3 is a receptor tyrosine kinase (RTK) that dimerizes on binding its ligand, the cytokine FLT3 ligand (FL), which then undergoes autophosphorylation, and transduces signals downstream (STAT, AKT, ERK) that promote proliferation and survival. Activating mutations of FLT3 are common mutations found in AML. A particular form of FLT3 is mutated by duplicating coding sequence derived from the juxtamenbrane domain inserted in tandem (internal tandem duplication (ITD). These FLT3-ITD mutations result in the constitutive activation of the tyrosine kinase function. Patients with FLT3-ITD mutations have increased relapse rates and reduced overall survival. Point mutations in the activation loop of the kinase domain, most commonly at the residue Asp 835 (D835), also known as tyrosine kinase domain (TKD) mutations, also result in the constitutive activation of the FLT3 kinase. Signaling from FLT3-TKD mutations does not appear as abnormal, and the prognostic impact appears to be less severe when compared with ITD mutations (FLT3-ITD mutations are found in ~23% of AML cases, whereas TKD mutations are found in 7%).
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 485010 | FLT3 Mutation Analysis | 485012 | FLT3 ITD Result | 79210-1 | ||
| 485010 | FLT3 Mutation Analysis | 485019 | FLT3 D835 Result | 72520-0 | ||
| 485010 | FLT3 Mutation Analysis | 485013 | Background | 77202-0 | ||
| 485010 | FLT3 Mutation Analysis | 485015 | Method | 49549-9 | ||
| 485010 | FLT3 Mutation Analysis | 485016 | References | 75608-0 | ||
| 485010 | FLT3 Mutation Analysis | 485017 | Director Review | 72486-4 |
© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.
CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf