Test Details
Methodology
Sensititre® broth microdilution (MIC)
Result Turnaround Time
21 - 28 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Susceptibility testing for amikacin, ciprofloxacin, clarithromycin, doxycycline, linezolid, minocycline, moxifloxacin, rifabutin, rifampin, streptomycin, trimethoprim/sulfamethoxazole. MIC values will be reported with CLSI interpretive comments if available.
Use
Determine the susceptibility of Mycobacterium marinum isolates to relevant antimycobacterial agents. Routine susceptibility testing of M. marinum isolates is generally not recommended.1,2 The following agents are typically found to be susceptible when testing M. marinum: Rifampin, Ethambutol, Doxycycline, Minocycline, Clarithromycin and Trimethoprim-sulfamethoxazole.3 Disease due to M. marinum is generally localized and the number of organisms is low, with 95% of tissue biopsies reported as AFB smear negative. Susceptibility testing should be considered when patients remain culture positive after several months of therapy.4
Limitations
Susceptibilities cannot be reported if the organism fails to grow in the test medium. Susceptibilities cannot be performed on mixed cultures.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Custom Additional Information
Few comparative studies have been performed relating clinical outcome with in vitro susceptibility testing. Ciprofloxacin is not recommended for therapy because some strains are resistant and with monotherapy, the risk of mutational resistance increases.
Specimen Requirements
Specimen
Mycobacterium marinum isolate from a primary clinical specimen should be submitted on conventional solid or broth AFB broth medium.
Volume
Pure culture isolate on an AFB conventional solid medium or a minimum of 1 mL of AFB broth medium
Container
Conventional or broth medium, tightly sealed, in etiologic agent packaging
Storage Instructions
Maintain media at room temperature.
Causes for Rejection
Specimen received in leaking or in broken transport tube or vial; specimen received in expired transport medium; mixed culture; unlabeled culture or name discrepancy between specimen and request label
Footnotes
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 182840 | M. marinum Susceptibility | 29576-6 | 182839 | Organism ID | 9825-1 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998549 | Amikacin | 5-Dec | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998707 | Ciprofloxacin | 185-9 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998550 | Clarithromycin | 189-1 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998246 | Clofazimine | 16623-1 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998720 | Doxycycline | 225-3 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998711 | Linezolid | 29254-0 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998552 | Minocycline | 335-0 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998712 | Moxifloxacin | 31037-5 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998241 | Rifabutin | 16100-0 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998553 | Rifampin | 428-3 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998714 | Streptomycin | 460-6 | |
| 182840 | M. marinum Susceptibility | 29576-6 | 998554 | Trimethoprim/Sulfa | 516-5 | |
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 182839 | |||||
| Result Code Name | Organism ID | |||||
| UofM | ||||||
| Result LOINC | 9825-1 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998549 | |||||
| Result Code Name | Amikacin | |||||
| UofM | ||||||
| Result LOINC | 5-Dec | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998707 | |||||
| Result Code Name | Ciprofloxacin | |||||
| UofM | ||||||
| Result LOINC | 185-9 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998550 | |||||
| Result Code Name | Clarithromycin | |||||
| UofM | ||||||
| Result LOINC | 189-1 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998246 | |||||
| Result Code Name | Clofazimine | |||||
| UofM | ||||||
| Result LOINC | 16623-1 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998720 | |||||
| Result Code Name | Doxycycline | |||||
| UofM | ||||||
| Result LOINC | 225-3 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998711 | |||||
| Result Code Name | Linezolid | |||||
| UofM | ||||||
| Result LOINC | 29254-0 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998552 | |||||
| Result Code Name | Minocycline | |||||
| UofM | ||||||
| Result LOINC | 335-0 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998712 | |||||
| Result Code Name | Moxifloxacin | |||||
| UofM | ||||||
| Result LOINC | 31037-5 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998241 | |||||
| Result Code Name | Rifabutin | |||||
| UofM | ||||||
| Result LOINC | 16100-0 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998553 | |||||
| Result Code Name | Rifampin | |||||
| UofM | ||||||
| Result LOINC | 428-3 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998714 | |||||
| Result Code Name | Streptomycin | |||||
| UofM | ||||||
| Result LOINC | 460-6 | |||||
| Order Code | 182840 | |||||
| Order Code Name | M. marinum Susceptibility | |||||
| Order Loinc | 29576-6 | |||||
| Result Code | 998554 | |||||
| Result Code Name | Trimethoprim/Sulfa | |||||
| UofM | ||||||
| Result LOINC | 516-5 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 000000 | Antimicrobial Susceptibility | 000000 | Antimicrobial Susceptibility | N/A | |
| Reflex 1 | ||||||
| Order Code | 000000 | |||||
| Order Name | Antimicrobial Susceptibility | |||||
| Result Code | 000000 | |||||
| Result Name | Antimicrobial Susceptibility | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||