Urine Culture, Routine

CPT: 87086
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Synonyms

  • Culture, Urine, Routine
  • Midstream Urine Culture, Routine
  • Routine Culture, Urine, Clean Catch
  • Urine Culture, Catheter
  • Urine Culture, Routine, Midvoid Specimen

Test Includes

Culture; quantitation, isolation, identification (additional charges/CPT code[s] may apply), and susceptibility testing of up to two organisms at >10,000 colonies/mL if culture results warrant (additional charges/CPT code[s] may apply). CPT coding for microbiology and virology procedures often cannot be determined before the culture is performed. Requests with only a written order and no test number indicated will be processed according to Default Testing for Routine Microbiology.


Special Instructions

Unrefrigerated, unpreserved urine specimens greater than two hours old may be subject to overgrowth with organisms normally present in the urethra and periurethral areas, and may yield inaccurate or misleading results. If specimens are incorrectly submitted with an order for aerobic bacterial culture, the laboratory will process the specimen for the test based on the source listed on the request form. The client will not be contacted to approve this change, but the change will be indicated on the report.


Expected Turnaround Time

2 - 4 days



Related Documents

For more information, please view the literature below.

Microbiology Specimen Collection and Transport Guide


Specimen Requirements


Specimen

Urine


Volume

To minimum fill line (4 mL) on Vacutainer® gray-top urine culture transport tube with preservative (preferred)


Container

Vacutainer® gray-top urine culture transport tube with preservative (preferred). If less than 4 mL of urine is collected, usually from pediatric and geriatric patients or from a catheter, submit refrigerated in a sterile, screw cap container or tube. Do not submit low volume urine specimens in underfilled gray top tubes.


Collection

Clean catch mid-stream collection. First morning specimens yield highest bacterial counts from overnight incubation in the bladder, and are the best specimens. Read Patient Preparation.


Storage Instructions

Preserved: Room temperature for 48 hours

Unpreserved: Refrigerated for 24 hours


Patient Preparation

Patient should be instructed on the proper collection of a clean catch midstream urine specimen. Avoid contamination with normal flora from skin, rectum or vagina. If a clean catch urine cannot be obtained from an infant, obtain a bagged specimen: clean area as for a clean catch, attach U-bag, and put collected urine into a sterile container.

Clean catch specimen: Have patient urinate into a small clean disposable cup (styrofoam or “Dixie”). Afterward, collection site staff can transfer the urine to the urine culture transport tube using the special collection straw-puncture device designed for use with the Vacutainer® tubes. The numbers of bacteria in a clean unused cup are so few as to be inconsequential when the urine transport stabilizer is added. Thoroughly instruct patient for proper collection of a “clean catch” specimen. Patient must be instructed to thoroughly cleanse skin and collect a midstream specimen. The patient should be instructed to follow the directions provided with the urine collection kit as follows.

Catheterized specimen: Refers to an “in and out” catheter that is placed into the bladder solely for collection of the specimen and then withdrawn. Do not collect urine from the drainage bag when an indwelling catheter is in place because growth of bacteria can occur in the bag itself. Rather, clean catheter with an alcohol sponge, puncture with a sterile needle, and collect in sterile syringe. Catheter tips are contaminated by the urethra as they are withdrawn; do not culture them.

Male: Wash hands thoroughly with soap and water. Rinse them well and dry with a paper towel.

• Tear open the towelette packages so that the towels can be easily removed with one hand as they are needed. Do not touch any of the inside surfaces of the collection cup.

• Pull back the foreskin to expose the head of the penis completely.

• Wash the head of the penis thoroughly using first one towelette then the other. Discard the used towelettes into the toilet bowl.

• Pass a small amount of urine into the toilet bowl, then pass a sample into the container. Do not allow the container to touch the legs or penis. Keep fingers away from the rim and inner surface of the container. Fill the container half full.

• The urine specimen should be transferred to the Vacutainer® tube within 10 minutes of collection.

Female: Wash hands thoroughly with soap and water. Rinse them well and dry with a paper towel.

• Tear open the towelette packages so that the towels can be easily removed with one hand as they are needed. Do not touch any of the inside surfaces of the collection cup.

• Remove undergarments and sit on the toilet seat with legs spread widely apart.

• With one hand, spread labia apart to expose the vulva. Keep this hand in place during the washing and urinating procedure.

• Use one towelette to wash the vulva well passing the towelette only from front to back, not back and forth. Repeat this procedure using the second towelette. Discard the used towelettes into the toilet bowl.

• Begin urinating into the toilet bowl then, without stopping the stream, insert the collection cup to collect the specimen. Do not allow the container to touch the legs, vulva, or clothing. Keep fingers away from the rim and inner surface of the container. Fill the container about half full.

• The urine specimen should be transferred to the Vacutainer® tube within 10 minutes of collection.


Causes for Rejection

Unrefrigerated, unpreserved specimen greater than two hours old; unlabeled specimen or name discrepancy between specimen and request label; specimen in expired transport container; specimen received after prolonged delay (usually more than 48 hours for urine); specimen collected from a Foley catheter bag; specimen in nonsterile or leaking container


Test Details


Use

Semiquantitative culture to isolate and identify bacterial causes of urinary tract infection. Detect up to two pathogenic bacterial organisms at levels above 10,000 cfu/mL.


Methodology

Culture


Additional Information

A single culture is about 80% accurate in the female; two containing the same organism with a count of 100,000 cfu/mL or more represent a 95% chance of true bacteriuria; three such specimens mean virtual certainty of true bacteriuria. A single clean voided specimen from an adult male may be considered diagnostic with proper preparation and care in specimen collection. If the patient is receiving antimicrobial therapy at the time the specimen is collected, any level of bacteriuria may be significant. When more than two organisms are recovered, the likelihood of contamination is high; thus, the significance of definitive identification of the organisms and susceptibility testing in this situation is severely limited. A repeat culture with proper specimen collection including patient preparation is often indicated. Cultures of specimens from Foley catheters yielding multiple organisms with high colony counts usually represents colonization of the catheter and not true significant bacteriuria. Failure to recover aerobic organisms from patients with pyuria or positive Gram stains of urinary sediment may indicate the presence of mycobacteria or anaerobes.


References

Cavagnolo R. Evaluation of incubation times for urine cultures. J Clin Microbiol. 1995 Jul;33(7):1954-1956.7665682
Joho KL, Soliman H, Weinstein MP. Comparison of one-day versus two-day incubation of urine cultures. Diagn Microbiol Infect Dis. 1995 Jan;21(1):55-56.7789098
McCarter YS, Burd EM, Hall GS, Zervos M. Laboratory diagnosis of urinary tract infections. Sharp SE, ed. Cumitech 2C, Washington, DC: ASM Press; 2009.
Ronald AR, Nicolle LE, Harding GKM. Standards of therapy for urinary tract infections in adults. Infection. 1992;20(Suppl 3):S164-S170.1490743
Stamm WE. Criteria for the diagnosis of urinary tract infection and for the assessment of therapeutic effectiveness. Infection. 1992;(Suppl 3):S151-S159.1490740

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
008847 Urine Culture, Routine 630-4 008847 Urine Culture, Routine 630-4
Reflex Table for Urine Culture, Routine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 997870 Result 997131 Result 1 630-4
Reflex Table for Urine Culture, Routine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 997870 Result 997132 Result 2 630-4
Reflex Table for Urine Culture, Routine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 997870 Result 997133 Result 3 630-4
Reflex Table for Urine Culture, Routine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 997870 Result 997134 Result 4 630-4
Reflex Table for Urine Culture, Routine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 997870 Result 997135 Antimicrobial Susceptibility 23658-8

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