Immunofixation (IFE), Urine

CPT: 86335
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Test Details


  • Electrophoresis, Urine, IFE
  • IEP, Urine
  • IFE, Urine
  • Immunoelectrophoresis, Urine
  • Urine Immunoelectrophoresis


Detect and identify monoclonal immunoglobulin gammopathies


Agarose gel immunofixation electrophoresis (IFE)

Reference Interval

Normal pattern

Additional Information

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will help identify the specific light and heavy chain components of a monoclonal protein.

Specimen Requirements


Urine (random or 24-hour)


10 mL aliquot

Minimum Volume

5 mL aliquot


Plastic urine container, no preservative


Random or 24-hour urine collection; mix well.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

State patient's age and clinical diagnosis on the test request form.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
123034 Immunofixation, Urine 13440-3 123035 IFE Interpretation:U 13440-3

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