Immunofixation (IFE), Urine

CPT: 86335
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Test Details

Synonyms

  • Electrophoresis, Urine, IFE
  • IEP, Urine
  • IFE, Urine
  • Immunoelectrophoresis, Urine
  • Urine Immunoelectrophoresis

Use

Detect and identify monoclonal immunoglobulin gammopathies

Methodology

Agarose gel immunofixation electrophoresis (IFE)

Reference Interval

Normal pattern

Additional Information

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will help identify the specific light and heavy chain components of a monoclonal protein.

Specimen Requirements

Specimen

Urine (random or 24-hour)

Volume

10 mL aliquot

Minimum Volume

5 mL aliquot

Container

Plastic urine container, no preservative

Collection

Random or 24-hour urine collection; mix well.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

State patient's age and clinical diagnosis on the test request form.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
123034 Immunofixation, Urine 13440-3 123035 IFE Interpretation:U 13440-3

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