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Immunofixation (IFE) and Protein Electrophoresis, Random Urine

CPT 84156; 84166; 86335
Synonyms
  • Bence Jones Protein Identification, Random Urine
  • Globulins, Random Urine
  • Myeloma Proteins, Random Urine
  • Urine Immunofixation for Myeloma Proteins

Test Details

Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

α1-Globulin, urine; α2-globulin, urine; β-globulin, urine; γ-globulin, urine; M-spike percentage; protein immunofixation, urine

Use

Characterize the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ-globulin, γ-globulin fragments (light chains, Bence Jones proteins); of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström, and amyloidosis; work up monoclonal gammopathy (M-spike)

Special Instructions

The request form should include patient's age and clinical diagnosis.

Specimen Requirements

Specimen

Urine (random) (For 24-hour urine specimens, order test 003467.)

Volume

30-mL aliquot of random urine

Minimum Volume

11-mL aliquot of random urine

Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative

Collection Instructions

Collect first morning urine; mix well.

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1

Storage Instructions

Refrigerated (preferred) or room temperature

Causes for Rejection

Preservative added

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
122390 IFE and PE, Random Urine 013664 Protein,Total,Urine mg/dL 2888-6
122390 IFE and PE, Random Urine 133751 Albumin, U % 13986-5
122390 IFE and PE, Random Urine 133769 Alpha-1-Globulin, U % 13984-0
122390 IFE and PE, Random Urine 133777 Alpha-2-Globulin, U % 13987-3
122390 IFE and PE, Random Urine 133785 Beta Globulin, U % 13994-9
122390 IFE and PE, Random Urine 133793 Gamma Globulin, U % 13989-9
122390 IFE and PE, Random Urine 133794 M-Spike, % % 42484-6
122390 IFE and PE, Random Urine 013482 Immunofixation Result, Urine 13440-3
122390 IFE and PE, Random Urine 149533 Note: N/A
122390 IFE and PE, Random Urine 003724 PDF 11502-2
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code013664
Result Code NameProtein,Total,Urine
UofMmg/dL
Result LOINC2888-6
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133751
Result Code NameAlbumin, U
UofM%
Result LOINC13986-5
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133769
Result Code NameAlpha-1-Globulin, U
UofM%
Result LOINC13984-0
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133777
Result Code NameAlpha-2-Globulin, U
UofM%
Result LOINC13987-3
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133785
Result Code NameBeta Globulin, U
UofM%
Result LOINC13994-9
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133793
Result Code NameGamma Globulin, U
UofM%
Result LOINC13989-9
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code133794
Result Code NameM-Spike, %
UofM%
Result LOINC42484-6
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code013482
Result Code NameImmunofixation Result, Urine
UofM
Result LOINC13440-3
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code149533
Result Code NameNote:
UofM
Result LOINCN/A
Order Code122390
Order Code NameIFE and PE, Random Urine
Order Loinc
Result Code003724
Result Code NamePDF
UofM
Result LOINC11502-2