Immunofixation (IFE), Serum and Protein Electrophoresis, Serum

CPT: 82784(x3) (IgG, IgA, IgM); 84155; 84165 (protein electrophoretic fractionation and quantitation); 86334 (immunofixation electrophoresis)
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Test Details

Synonyms

  • Globulins, Serum
  • IEP, Serum
  • IFE, Serum and Protein Electrophoresis, Serum
  • Immunoelectrophoresis, Serum
  • Protein IEP

Test Includes

Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of heavy- and light-chain patterns; serum protein electrophoresis

Use

Detect and identify monoclonal immunoglobulin gammopathies

Methodology

Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); protein electrophoresis on agarose media; turbidimetric quantitation of IgA, IgG, and IgM

Reference Interval

Normal pattern

Additional Information

Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states.

Specimen Requirements

Specimen

Serum

Volume

5 mL

Minimum Volume

2 mL

Container

Red-top tube or gel-barrier tube

Patient Preparation

Patient should be fasting.

Collection

Separate serum from cells.

Storage Instructions

Refrigerate.

Stability Requirements

Temperature

Period

Room temperature

3 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross hemolysis; gross lipemia

Clinical Information

Special Instructions

State the patient's age and clinical diagnosis on the test request form.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001495 IFE and PE, Serum 001776 Immunoglobulin G, Qn, Serum mg/dL 2465-3
001495 IFE and PE, Serum 001784 Immunoglobulin A, Qn, Serum mg/dL 2458-8
001495 IFE and PE, Serum 001792 Immunoglobulin M, Qn, Serum mg/dL 2472-9
001495 IFE and PE, Serum 001073 Protein, Total, Serum g/dL 2885-2
001495 IFE and PE, Serum 149520 Albumin g/dL 2862-1
001495 IFE and PE, Serum 149521 Alpha-1-Globulin g/dL 2865-4
001495 IFE and PE, Serum 149522 Alpha-2-Globulin g/dL 2868-8
001495 IFE and PE, Serum 149523 Beta Globulin g/dL 2871-2
001495 IFE and PE, Serum 149524 Gamma Globulin g/dL 2874-6
001495 IFE and PE, Serum 149525 M-Spike g/dL 33358-3
001495 IFE and PE, Serum 149530 Globulin, Total g/dL 2336-6
001495 IFE and PE, Serum 149531 A/G Ratio 1759-0
001495 IFE and PE, Serum 149519 Immunofixation Result, Serum 13169-8
001495 IFE and PE, Serum 149534 Please note: N/A
001495 IFE and PE, Serum 001498 PDF N/A
Reflex Table for Immunofixation Result, Serum
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 149532 Immunofixation Interp.,Sr 149532 Immunofixation Interp.,Sr 13169-8

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