Immunofixation (IFE), Serum

CPT: 82784(x3) (IgG, IgA, IgM); 86334 (immunofixation electrophoresis)
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  • Electrophoresis, Serum, IFE
  • Immunoelectrophoresis, Serum

Test Includes

Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of monoclonal heavy- (IgG, IgA, IgM, IgD, IgE) and light-chain (kappa, Lambda) patterns, if present in the serum

Special Instructions

State patient's age and clinical diagnosis on the test request form.

Expected Turnaround Time

3 - 5 days

Related Documents

For more information, please view the literature below.

Published Plasma Cell Dyscrasias Selection Table

Specimen Requirements




2 mL

Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube


Separate serum from red cells.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Patient Preparation

Overnight fasting is preferred, but not required.

Causes for Rejection

Plasma sample

Test Details


Detect and identify monoclonal immunoglobulin gammopathies


Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); turbidimetric quantitation of IgA, IgG, and IgM

Reference Interval

Normal pattern

Additional Information

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001685 Immunofixation, Serum 34550-4 001795 Immunofixation Result, Serum 14895-7
001685 Immunofixation, Serum 34550-4 001776 Immunoglobulin G, Qn, Serum mg/dL 2465-3
001685 Immunofixation, Serum 34550-4 001784 Immunoglobulin A, Qn, Serum mg/dL 2458-8
001685 Immunofixation, Serum 34550-4 001792 Immunoglobulin M, Qn, Serum mg/dL 2472-9

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