Immunofixation (IFE) and Protein Electrophoresis, 24-Hour Urine

CPT: 84156; 84166; 86335
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Test Details

Synonyms

  • Bence Jones Protein Identification, 24-Hour Urine
  • Globulins, 24-Hour Urine
  • IFE/Protein Electrophoresis, 24-Hour Urine
  • Myeloma Proteins, 24-Hour Urine

Test Includes

Urine immunofixation; qualitative identification of light chains and urine protein electrophoresis

Use

Useful in characterizing the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ globulin, γ globulin fragments (light chains, Bence Jones proteins). Of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström and amyloidosis. Monoclonal gammopathy (M spike) work-up.

Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

Reference Interval

Normal pattern

Specimen Requirements

Specimen

Urine (24-hour); use test 122390 if random urine is obtained.

Volume

30-mL aliquot

Minimum Volume

11-mL aliquot

Container

12-mL plastic transport tube (LabCorp N° 23597) without preservative

Collection

Collect 24-hour urine; mix well.

Storage Instructions

Refrigerate.

Causes for Rejection

Preservative added

Clinical Information

Special Instructions

The request form should include patient's age and clinical diagnosis.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003467 IFE+Protein Electro, 24-Hr Ur 013664 Protein,Total,Urine mg/dL 2888-6
003467 IFE+Protein Electro, 24-Hr Ur 013219 Prot,24hr calculated mg/24 hr 2889-4
003467 IFE+Protein Electro, 24-Hr Ur 133751 Albumin, U % 13986-5
003467 IFE+Protein Electro, 24-Hr Ur 133769 Alpha-1-Globulin, U % 13984-0
003467 IFE+Protein Electro, 24-Hr Ur 133777 Alpha-2-Globulin, U % 13987-3
003467 IFE+Protein Electro, 24-Hr Ur 133785 Beta Globulin, U % 13994-9
003467 IFE+Protein Electro, 24-Hr Ur 133793 Gamma Globulin, U % 13989-9
003467 IFE+Protein Electro, 24-Hr Ur 133794 M-Spike, % % 42484-6
003467 IFE+Protein Electro, 24-Hr Ur 133796 M-Spike, mg/24 hr mg/24 hr 42482-0
003467 IFE+Protein Electro, 24-Hr Ur 013482 Immunofixation Result, Urine 13440-3
003467 IFE+Protein Electro, 24-Hr Ur 149533 Note: N/A
003467 IFE+Protein Electro, 24-Hr Ur 003724 PDF N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf