Cyclospora Smear, Stool

CPT: 87015; 87206
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Synonyms

  • Cyclospora cayetanensis
  • CLB Smear

Test Includes

Examination of oocysts of Cyclospora


Expected Turnaround Time

3 - 6 days



Related Documents

For more information, please view the literature below.

Microbiology Specimen Collection and Transport Guide


Specimen Requirements


Specimen

Stool


Volume

5 g


Container

Parasite (O & P) transport with formalin (Para-Pak® pink)


Collection

Fecal specimens for parasitic examination should be collected before initiation of antidiarrheal therapy or antiparasitic therapy. The highest yield on hospitalized patients occurs when diarrhea is present on admission or within 72 hours of admission. The onset of diarrhea more than 72 hours after admission is usually caused by Clostridium difficile toxin rather than parasites or the usual stool pathogens. The following recommendations are made for efficient and cost-effective diagnosis of diarrheal disease in patients admitted with gastroenteritis.

• Submit one or two specimens per diarrheal illness immediately. Consider requesting the EIA for Giardia (see Giardia lamblia, EIA and Ova and Parasites Examination [188110] and Giardia lamblia, Direct Detection EIA [182204]) if that is the primary suspected organism.

• If those are negative, submit an additional specimen after five days.

• Patients who are immunocompromised by AIDS, malignancy, or immunosuppressive therapy may require additional testing for unusual stool pathogens (eg, Cryptosporidium, Direct Detection EIA [183020]).


Storage Instructions

Maintain specimen at room temperature.


Causes for Rejection

Inappropriate specimen transport conditions (eg, frozen) or device; improper labeling; specimen received after prolonged delay (usually more than five days); leaking specimen; specimen received in denture cup, “Cool Whip” container, margarine container, or similar container


Test Details


Use

Detect Cyclospora


Limitations

The organisms are most readily demonstrated in diarrheal stools rather than formed stools.


Methodology

Modified Kinyoun stain and autofluorescence


Reference Interval

No Cyclospora oocysts observed


Additional Information

Cyclospora cayetanensis is a coccidian parasite first associated with human infection in 1977. Before 1966, there had been documentation of only a few outbreaks. In 1966, there were outbreaks in 10 states and Canada that were associated with the consumption of raspberries. More than 850 laboratory confirmed cases were reported to the Centers for Disease Control and Prevention (CDC) in May and June, 1996.


References

Centers for Disease Control and Prevention. Outbreaks of Cyclospora cayetanensis infection−United States, 1996. MMWR. 1996 Jun 28; 45(25):549-551. 9132573
Centers for Disease Control and Prevention. Update: Outbreaks of Cyclospora cayetanensis infection−United States and Canada, 1996. MMWR. 1996 Jul 19; 45(28):611-613. 8676854

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183145 Cyclospora Smear, Stool 10659-1 183146 Cyclospora Smear, Stool 10659-1

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