Antinuclear Ab Profile, 11-biomarker Profile, by Multiplex Immunoassay, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B, Sm/RNP, Ribosomal P

CPT: 83516; 86225; 86235(x9)
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Synonyms

  • ANA Comprehensive Plus Profile

Test Includes

Centromere B; chromatin; dsDNA; Jo-1; ribosomal P; RNP; scl-70; Sjögren's A; Sjögren's B; Sm/RNP; Smith (Sm)


Special Instructions

Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855].


Expected Turnaround Time

2 - 3 days


Related Documents


Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination; icterus


Test Details


Use

Detect antibodies associated with SLE and mixed connective tissue disease. Sm antibodies are highly specific for SLE. RNP antibodies are found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Autoantibodies to the ribosomal P protein represent a highly specific marker for the diagnosis of SLE, where they have been associated with certain clinical manifestations. The native protein SmRNP contains two different regions: Sm and RNP that are different regions of the same macromolecule. SmRNP is the best antigen for detection of antibodies to either Sm or RNP but cannot discriminate between the antibody specificities. Inclusion of SmRNP increases sensitivity while inclusion of Sm and RNP increases specificity.


Methodology

Multiplex flow immunoassay


References

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Eriksson C, Engstrand S, Sundqvist KG, Rantapää-Dahlqvist S. Autoantibody formation in patients with rheumatoid arthritis treated with anti-TNF alpha. Ann Rheum Dis. 2005 Mar; 64(3):403-407. 15297281
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997 Sep; 43(9):1749-1756. 9299971
Golightly M, Golightly C. Laboratory diagnosis of autoimmune diseases. MLO Med Lab Obs. 2002 Jul; 34(7):12-16, 20. 12134551
Homburger HA, Cahen YD, Griffiths J, Jacob GL. Detection of antinuclear antibodies: Comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. Arch Pathol Lab Med. 1998 Nov; 122(11):993-999. 9822128
Isenberg DA. Systemic lupus erythematosus and Sjögren's syndrome: Historical perspective and ongoing concerns. Arthritis Rheum. 2004 Mar; 50(3):681-683. 15022304
Kallenberg CG, Wouda AA, Hoet MH, van Venrooij WJ. Development of connective tissue disease in patients presenting with Raynaud's phenomenon: A six year follow-up with emphasis on the predictive value of antinuclear antibodies as detected by immunoblotting. Ann Rheum Dis. 1988 Aug; 47(8):634-641. 3261966
Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med. 2000 Jan; 124(1):71-81. 10629135
Narain S, Richards HB, Satoh M, et al. Diagnostic accuracy for lupus and other systemic autoimmune diseases in the primary care setting. Arch Intern Med. 2004 Dec 13-27; 164(22):2435-2441. 15596633
Phan TG, Wong RC, Adelstein S. Autoantibodies to extractable nuclear antigens: Making detection and interpretation more meaningful. Clin Diagn Lab Immunol. 2002 Jan; 9:(1):1-7. 11777822
Reeves WH, Satoh M. Autoantibodies in systemic lupus erythematosus. In: Koopman WJ, ed, Arthritis and Allied Conditions: A Textbook of Rheumatology. 14th ed. Philadelphia, Pa: Lippincott, Williams and Wilkins; 2001:1483.
Reichlin M, Harley JB. Antinuclear antibodies: An overview. In: Wallace DJ, Hahn BH, eds. Dubois Lupus Erythematosus. 5th ed. Baltimore, Md: Williams and Wilkins;1997:403.
Reisner BS, DiBlasi J, Goel N. Comparison of an enzyme immunoassay to an indirect fluorescent immunoassay for the detection of antinuclear antibodies. Am J Clin Pathol. 1999 Apr; 111(4):503-506. 10191770
Rekvig OP, Kalaaji M, Nossent H. Anti-DNA antibody subpopulations and lupus nephritis. Autoimmun Rev. 2004 Feb; 3(2):1-6. 15003181
Simón JA, Cabiedes J, Ortiz E, Alcocer-Varela J, Sánchez-Guerrero J. Anti-nucleosome antibodies in patients with systemic lupus erythematosus of recent onset. Potential utility as a diagnostic tool and disease activity marker. Rheumatology (Oxford). 2004 Feb; 43(2):220-224. 13130152
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LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164920 Antinuclear Ab 11 by Multiplex 096343 Anti-DNA (DS) Ab Qn IU/mL 5130-0
164920 Antinuclear Ab 11 by Multiplex 016354 RNP Antibodies AI 29374-6
164920 Antinuclear Ab 11 by Multiplex 016362 Smith Antibodies AI 11090-8
164920 Antinuclear Ab 11 by Multiplex 016376 Smith/RNP Antibodies AI 27048-8
164920 Antinuclear Ab 11 by Multiplex 018707 Antiscleroderma-70 Antibodies AI 27416-7
164920 Antinuclear Ab 11 by Multiplex 012684 Sjogren's Anti-SS-A AI 17792-3
164920 Antinuclear Ab 11 by Multiplex 012692 Sjogren's Anti-SS-B AI 17791-5
164920 Antinuclear Ab 11 by Multiplex 012581 Antichromatin Antibodies AI 51775-5
164920 Antinuclear Ab 11 by Multiplex 012701 Antiribosomal P Antibodies AI 13636-6
164920 Antinuclear Ab 11 by Multiplex 161457 Anti-Jo-1 AI 11565-9
164920 Antinuclear Ab 11 by Multiplex 164816 Anti-Centromere B Antibodies AI 53982-5
164920 Antinuclear Ab 11 by Multiplex 164879 See below: N/A

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