NeuroSURE® Metabolites: Neurotransmitter Metabolites (5 HIAA, HVA, 30MD) (CSF)

CPT: 82542; 83150; 83497

Expected Turnaround Time

10 - 14 days

Specimen Requirements


Cerebrospinal fluid (CSF)


0.5 mL

Minimum Volume

0.25 mL


Sterile screw capped vial


Collect from first drops of lumbar puncture into single sterile tube. Do not aliquot from a pooled sample.

Storage Instructions

Freeze as soon as possible.

Stability Requirements



Room temperature

Unstable (stability provided by manufacturer or literature reference)


24 hours (stability provided by manufacturer or literature reference)


-20°C = 72 hours; -80°C = Indefinite (stability provided by manufacturer or literature reference)

Causes for Rejection

Bloody CSF; received thawed

Test Details


CSF Neurotransmitter Metabolites (5HIAA, HVA, 3OMD) (NC04) is useful for diagnosis of certain disorders of neurotransmitter metabolism. This testing may also be used for assessment of Variants of Uncertain Significance (VUS) identified during genetic testing (e.g. Next Generation Sequencing or Capillary Sequencing testing). Monoamine metabolite testing includes homovanillic acid (HVA), 3-O-methyl-Dopa (3-OMD), and 5-hydroxyindole acetic acid (5-HIAA). This test is useful in diagnosing pediatric neurotransmitter diseases affecting dopamine and serotonin metabolism in the brain. Inborn errors of metabolism and and various drugs may lead to severe imbalances and disturbances in these neurotransmitter systems that are reflected by changes in the concentration of monoamines metabolites in CSF. Primary inherited defects involve deficiencies in tyrosine and tryptophan hydroxylase, aromatic amino acid decarboxylase, monoamine oxidase, dopamine beta hydroxylase and the dopamine transwporter. Other defects in the biopterin synthesis pathway may also affect dopamine and serotonin metabolism. These disorders are characterized by a wide range of symptoms that may include developmental delay, mental disability, behavioral disturbances, dystonia, seizures, encephalopathy, athetosis and ptosis.


Qualitative results if received in pooled sample.

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


High-pressure liquid chromatography (HPLC)

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