Hepatitis C Virus (HCV), Quantitative, Real-time PCR (Graphical)

CPT: 87522
Updated on 03/10/2024
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Synonyms

  • HCV RNA Quantitation, TaqMan®

Special Instructions

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, order Hepatitis C Virus (HCV), Quantitative, Real-time PCR (Nongraphical) [550080].

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, order 550080.

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, order Hepatitis C Virus (HCV), Quantitative, Real-time PCR (Nongraphical) [550080].


Expected Turnaround Time

3 - 5 days



Specimen Requirements


Specimen

Serum or plasma


Volume

2.5 mL


Minimum Volume

700 μL (Note: This volume does not allow for repeat testing.)


Container

Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™)


Collection

Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.


Storage Instructions

Freeze (preferred) or refrigerate.

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for 72 hours. Stable frozen for six weeks.

Freeze (preferred) or refrigerate.


Stability Requirements

Temperature

Period

Room temperature

24 hours (stability provided by manufacturer or literature reference)

Refrigerated

72 hours (stability provided by manufacturer or literature reference)

Frozen

6 weeks (stability provided by manufacturer or literature reference)

Temperature

Period

Room temperature

24 hours (stability provided by manufacturer or literature reference)

Refrigerated

72 hours (stability provided by manufacturer or literature reference)

Frozen

6 weeks (stability provided by manufacturer or literature reference)


Causes for Rejection

Incorrect anticoagulant; PPT™ not centrifuged


Test Details


Use

Determine the number of international units (IU) of hepatitis C virus (HCV) RNA per milliliter in serum or plasma in known HCV-positive patients


Limitations

The quantifiable range of the assay is 15 IU/mL to 100,000,000 IU/mL.


Methodology

Cobas® HCV (Roche Molecular Systems)

Cobas® AmpliPrep/Cobas® TaqMan® HCV Test v 2.0

Cobas® HCV (Roche Molecular Systems)


References

Yao JD, Young S, Heilek GM, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018 May;102:63-69.29518694
Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521
Yao JD, Young S, Heilek GM, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018 May;102:63-69.29518694

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550070 HCV RT-PCR, Quant (Graph) 11011-4 550266 Hepatitis C Quantitation IU/mL 11011-4
550070 HCV RT-PCR, Quant (Graph) 11011-4 550259 HCV log10 log10 IU/mL 38180-6
550070 HCV RT-PCR, Quant (Graph) 11011-4 550307 Test Information: N/A
550070 HCV RT-PCR, Quant (Graph) 11011-4 511958 PDF 80563-0
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550265 HCV RNA (International Units) 550263 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550265 HCV RNA (International Units) 550264 HCV log10 log10 IU/mL 38180-6

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