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Glomerulonephritis (GNP) Profile (RDL)

CPT: 83520(x3); 86038; 86160(x2); 86225; 86255

Test Includes

This profile is composed of Anti-Nuclear Ab (ANA) by IFA, Anti-dsDNA Ab by Farr, Anti-Glomerular Basement Membrane Ab (GBM), Anti-Neutrophil Cytoplasmic Ab (ANCA), Anti-Myeloperoxidase Ab (MPO), Anti-Proteinase 3 Ab (PR-3), C3 Complement, C4 Complement.

ANA performed if ANCA positive to rule out false positive ANCA due to presence of ANA.

Expected Turnaround Time

14 - 21 days

Specimen Requirements




4 mL

Minimum Volume

1.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube


Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Refrigerate or freeze.

Stability Requirements



Room temperature

2 days


8 days


8 days

Test Details


ANA are commonly present in a variety of autoimmune diseases. ANA frequency also increases with age in apparently healthy people. ANA patterns on HEp-2 slides provide general clues about particles (chromatin, nucleosomes, and spliceosomes).

The Farr assay is the most specific method for detecting dsDNA autoantibodies. Significant elevations in dsDNA autoantibody concentrations confirm the diagnosis of systemic lupus erythematosus (SLE). Serial studies of elevated values of dsDNA auto antibodies are useful for predicting activity of SLE.

Anti-MPO autoantibodies aid in assessment of certain autoimmune vasculitides such as microscopic polyarteritis, and crescentic glomerulonephritis.

Elevated levels of Anti-PR-3 antibodies are classically observed in patients with Wegener granulomatosis (WG), particularly with active disease, and less frequently in other forms of necrotizing vasculitis.

Anti-GBM autoantibodies are recognized as being important in the pathogenesis of the rapidly progressive glomerulonephritis of Goodpasture's syndrome. Measurements of C3 and C4 are used to detect individuals with inborn deficiency of this factor or those with immunologic disease in whom complement is consumed at an increased rate. These include lupus erythematosus, chronic active hepatitis, certain chronic infections, post-streptococcal and membranoproliferative glomerulonephritis, and others.


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CPT Statement/Profile Statement

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