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ANA 12 Plus Profile, Do All (RDL)

CPT: 86038; 86147(x3); 86160(x2); 86200; 86225; 86235(x6); 86256; 86376; 86431


  • ANA 12 Plus
  • ANA 12 Plus Panel

Test Includes

This profile is comprised of Anti‐Nuclear Ab (ANA) by IFA, Anti‐dsDNA Ab by Farr, Anti‐ENA Abs (Anti‐Sm & Anti‐RNP), Anti‐Centromere Ab, C3 & C4 Complements, Anti‐Ro (SS‐A) Ab, Anti‐La (SS‐B) Ab, Anti‐Scl‐70 Ab, Anti‐cardiolipin Abs (IgG, IgA & IgM isotypes), Anti‐TPO (Thyroid Microsomal Peroxidase) Ab, Anti‐Chromatin Ab, Rheumatoid Factor by Turb, Anti‐CCP (Cyclic Citrullinated Peptide) Ab.

The reflexive ANA 12 Plus Profile is a separate test (use 520180).

Expected Turnaround Time

7-14 days

Specimen Requirements




4 mL

Minimum Volume

1.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube


Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Refrigerate or freeze.

Stability Requirements



Room temperature

1 day


8 days


8 days

Causes for Rejection

Grossly hemolyzed, bacterial contamination, lipemic, icteric, non‐serum specimen types

Test Details


The ANA test detects autoantibodies that bind to the nuclear components of HEp‐2 cells. A positive ANA titer is not specific, but indicates the presence of one or more autoantibodies. Profiles of specific autoantibodies have diagnostic andpotential prognostic utility for several autoimmune diseases.

RDL’s ANA 12 Plus Profile Helps Differentiate Between 8 Autoimmune Disease States:


• ANA by IFA

Tests with high specificity

• Anti‐dsDNA by Farr

• Anti‐SM

Tests with moderate specificity

• Anti‐Ro

• Anti‐La

• Anti‐Chromatin

• C3 & C4 Complements

• Anticardiolipin IgG, IgA & IgM

Drug‐induced SLE

• Anti‐Chromatin

Sjogren’s Syndrome

• Anti‐Ro

• Anti‐La

Rheumatoid Arthritis

• Anti‐CCP (high specificity)

• Rheumatoid Factor (Turbidimetry)

Mixed Connective Tissue Disease (MCTD)


This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


ELISA, IFA, Farr RIA, Turbidimetry, CMIA

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2020, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at Additional information regarding LOINC® codes can be found at, including the LOINC Manual, which can be downloaded at