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Anti-Neutrophil Cytoplasmic Ab (ANCA) by Indirect Immunofluorescence (IFA) (RDL)

Test Number 520090
Test number copied
CPT 86036
Synonyms
  • ANCA Ab
  • Anti-Neutrophil Cytoplasmic Ab (ANCA)

Test Details

Methodology

Indirect Immunofluorescence (IFA)

Result Turnaround Time

5 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

This test reflexes to Anti-Nuclear Ab by IFA (RDL) if ANCA is positive in order to rule out false positive ANCA due to the presence of ANA.

Use

Although C-ANCA and P-ANCA are typically of the IgG isotype, C-ANCA are frequently reactive with PR-3. P-ANCA frequently react with MPO. ANCA/MPO antibodies are found in 30% in glomerular basement membrane disease (Wegener's Disease). 88% of patients with vasculitis-associated pulmonary hemorrhage have elevated IgM ANCA, whereas no patients who are IgM ANCA-negative have pulmonary hemorrhage. IgA ANCA is associated with Cholangitis, Kawasaki disease, Cystic fibrosis and Henoch-Schonlein purpura.

Limitations

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.3 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection Instructions

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple test on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1

Reference Range

Negative

Storage Instructions

Refrigerate or freeze.

Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
520090 ANCA by IFA (RDL) 35279-9 520071 ANCA by IFA (RDL) 35279-9
Order Code520090
Order Code NameANCA by IFA (RDL)
Order Loinc35279-9
Result Code520071
Result Code NameANCA by IFA (RDL)
UofM
Result LOINC35279-9
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520187 C-ANCA (IFA-ETOH) 14277-8
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520187
Result NameC-ANCA (IFA-ETOH)
UofM
Result LOINC14277-8
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520189 P-ANCA (IFA-ETOH) 14278-6
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520189
Result NameP-ANCA (IFA-ETOH)
UofM
Result LOINC14278-6
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520191 ANCA (IFA-Formalin) 17357-5
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520191
Result NameANCA (IFA-Formalin)
UofM
Result LOINC17357-5
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520074 Homogeneous Pattern 20398-4
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520074
Result NameHomogeneous Pattern
UofM
Result LOINC20398-4
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520076 Nucleolar Pattern 20399-2
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520076
Result NameNucleolar Pattern
UofM
Result LOINC20399-2
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520233 Speckled Pattern 20401-6
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520233
Result NameSpeckled Pattern
UofM
Result LOINC20401-6
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520234 Centromere Pattern 53993-2
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520234
Result NameCentromere Pattern
UofM
Result LOINC53993-2
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520235 Spindle Apparatus Pattern 53003-0
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520235
Result NameSpindle Apparatus Pattern
UofM
Result LOINC53003-0
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520236 Nuclear Membrane Pattern 54006-2
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520236
Result NameNuclear Membrane Pattern
UofM
Result LOINC54006-2
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520237 Midbody Pattern 54155-7
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520237
Result NameMidbody Pattern
UofM
Result LOINC54155-7
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520238 Nuclear Dot Pattern 53998-1
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520238
Result NameNuclear Dot Pattern
UofM
Result LOINC53998-1
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520239 PCNA Pattern 53006-3
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520239
Result NamePCNA Pattern
UofM
Result LOINC53006-3
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520240 Centriole Pattern 53993-2
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520240
Result NameCentriole Pattern
UofM
Result LOINC53993-2
Reflex Table for ANCA by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520245 Positive ANCA Reflex 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex 2 520248 ANA Panel for ANCA 520241 Note: 77202-0
Reflex 1
Order Code520245
Order NamePositive ANCA Reflex
Result Code520073
Result NameAnti-Nuclear Ab by IFA (RDL)
UofM
Result LOINC42254-3
Reflex 2
Order Code520248
Order NameANA Panel for ANCA
Result Code520241
Result NameNote:
UofM
Result LOINC77202-0