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CML FISH Reflex to JAK2V617F Mutation Analysis, Quantitative, With Reflex to CALR Mutation Analysis, JAK2 Exon 12-15 Mutation Analysis and MPL Mutation Analysis by NGS

Test Number 512221
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CPT

Contact CPT coding department at 800-222-7566, ext. 6-8400.

 
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  • Updated on 04/03/2025

Test Details

Methodology

Cytogenetics; fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

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  • Oncology Appendix

Use

This test is used to confirm the diagnosis of CML, establish the chronic phase karyotype for comparison with blast crisis alterations and monitor residual disease.

Special Instructions

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Please direct any questions regarding this test to oncology customer service at 800-345-4363. If reflex test is performed, additional charges/CPT code(s) may apply.

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Bone marrow or whole blood

Bone marrow or peripheral blood

Bone marrow or whole blood

Volume

Bone marrow: 2 mL in pediatric green-top (heparin) tube; peripheral blood: 3 mL in pediatric green-top (heparin) tube

Minimum Volume

1 mL bone marrow or 1 mL whole blood (Note: Minimum volumes may not allow for repeat testing.)

1 mL bone marrow or 1 mL whole blood (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top sodium-heparin tube

Pediatric green-top (heparin) tube

Green-top sodium-heparin tube

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

Submit at room temperature using Leukemia/Lymphoma Specimen Transport Kit (supplied by Labcorp). Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the test request form.

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Maintain specimen at room temperature.

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
512221 CML FISH Rfx JAK2 Rfx MPN NGS 150341 Cells Counted 62361-1
512221 CML FISH Rfx JAK2 Rfx MPN NGS 150342 Cells Analyzed 62360-3
512221 CML FISH Rfx JAK2 Rfx MPN NGS 150343 FISH Result 51867-0
512221 CML FISH Rfx JAK2 Rfx MPN NGS 510974 Interpretation 50398-7
512221 CML FISH Rfx JAK2 Rfx MPN NGS 150345 Specimen Type 31208-2
512221 CML FISH Rfx JAK2 Rfx MPN NGS 510976 Director Review 48672-0
512221 CML FISH Rfx JAK2 Rfx MPN NGS 512245 Reflex to JAK2 V617F N/A
512221 CML FISH Rfx JAK2 Rfx MPN NGS 512123 PDF 11502-2
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code150341
Result Code NameCells Counted
UofM
Result LOINC62361-1
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code150342
Result Code NameCells Analyzed
UofM
Result LOINC62360-3
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code150343
Result Code NameFISH Result
UofM
Result LOINC51867-0
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code510974
Result Code NameInterpretation
UofM
Result LOINC50398-7
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code150345
Result Code NameSpecimen Type
UofM
Result LOINC31208-2
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code510976
Result Code NameDirector Review
UofM
Result LOINC48672-0
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code512245
Result Code NameReflex to JAK2 V617F
UofM
Result LOINCN/A
Order Code512221
Order Code NameCML FISH Rfx JAK2 Rfx MPN NGS
Order Loinc
Result Code512123
Result Code NamePDF
UofM
Result LOINC11502-2
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489532 Specimen Type 66746-9
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489532
Result NameSpecimen Type
UofM
Result LOINC66746-9
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489556 JAK2 V617F Result 43399-5
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489556
Result NameJAK2 V617F Result
UofM
Result LOINC43399-5
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489557 JAK2 V617F % % 53761-3
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489557
Result NameJAK2 V617F %
UofM%
Result LOINC53761-3
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489560 CALR Result 77174-1
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489560
Result NameCALR Result
UofM
Result LOINC77174-1
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489561 CALR % % N/A
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489561
Result NameCALR %
UofM%
Result LOINCN/A
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489562 CALR Nucleotide 48004-6
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489562
Result NameCALR Nucleotide
UofM
Result LOINC48004-6
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489563 CALR Amino Acid Pending
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489563
Result NameCALR Amino Acid
UofM
Result LOINCPending
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489564 MPL Result 75033-1
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489564
Result NameMPL Result
UofM
Result LOINC75033-1
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489565 MPL % % N/A
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489565
Result NameMPL %
UofM%
Result LOINCN/A
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489566 MPL Nucleotide 48004-6
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489566
Result NameMPL Nucleotide
UofM
Result LOINC48004-6
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489567 MPL Amino Acid 48005-3
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489567
Result NameMPL Amino Acid
UofM
Result LOINC48005-3
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489568 E12-15 Result 48726-4
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489568
Result NameE12-15 Result
UofM
Result LOINC48726-4
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489569 E12-15 % % N/A
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489569
Result NameE12-15 %
UofM%
Result LOINCN/A
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489570 E12-15 Nucleotide 48004-6
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489570
Result NameE12-15 Nucleotide
UofM
Result LOINC48004-6
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489559 Reflex N/A
Reflex 2 489577 CALR + MPL + E12-E15 489571 E12-15 Amino Acid 48005-3
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489559
Result NameReflex
UofM
Result LOINCN/A
Reflex 2
Order Code489577
Order NameCALR + MPL + E12-E15
Result Code489571
Result NameE12-15 Amino Acid
UofM
Result LOINC48005-3
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489558 V617F Rfx CALR/MPL/E12-15 Bkgd 62364-5
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489558
Result NameV617F Rfx CALR/MPL/E12-15 Bkgd
UofM
Result LOINC62364-5
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489573 Method 49549-9
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489573
Result NameMethod
UofM
Result LOINC49549-9
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489574 References 75608-0
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489574
Result NameReferences
UofM
Result LOINC75608-0
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489575 Director Review 72486-4
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489575
Result NameDirector Review
UofM
Result LOINC72486-4
Reflex Table for Reflex to JAK2 V617F
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 489555 JAK2 V617F rfx CALR/MPL/E12-15 489576 NGS JAK2 Rfx CALR/MPL/E12-15 N/A
Reflex 1
Order Code489555
Order NameJAK2 V617F rfx CALR/MPL/E12-15
Result Code489576
Result NameNGS JAK2 Rfx CALR/MPL/E12-15
UofM
Result LOINCN/A