25-Hydroxy Vitamin D (D2+D3 Fractionated), LC/MS-MS

CPT: 82306
Print Share


  • Vitamin D, 25-Hydroxy, LC/MS-MS

Expected Turnaround Time

4 - 10 days

Related Documents

Specimen Requirements


Serum (preferred) or plasma


1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube


Serum or plasma must be separated from cells within 45 minutes of venipuncture. Submit serum or plasma in a plastic transport tube.

Storage Instructions

Freeze. Stable frozen for 34 months. Stable at room temperature or refrigerated for seven days.

Test Details


25-Hydroxy vitamin D is used to measure vitamin D status; it includes results for total 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3. This 25-hydroxy vitamin D assay and the alternative immunoassay test (Vitamin D, 25-Hydroxy [081950]) are both CDC-certified for accuracy.1


Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Additional Information

The biological function of vitamin D is to maintain normal levels of calcium and phosphorus absorption. 25-Hydroxy vitamin D is the storage form of vitamin D. Vitamin D assists in maintaining bone health by facilitating calcium absorption. Vitamin D deficiency can also cause osteomalacia, which frequently affects elderly patients. Vitamin D from sunshine on the skin or from dietary intake is converted predominantly by the liver into 25-hydroxy vitamin D, which has a long half-life and is stored in the adipose tissue. The metabolically active form of vitamin D, 1,25-di-hydroxy vitamin D, which has a short life, is then synthesized in the kidney as needed from circulating 25-hydroxy vitamin D. The reference interval of greater than 30 ng/mL is a target value established by the Endocrine Society.2


1. Centers for Disease Control and Prevention (CDC). Laboratory Quality Assurance and Standardization Programs. Standardization of Measurement Procedures. Vitamin D Standardization Certification Program (VDSCP). Atlanta, Ga: CDC;2015. Available at: http://www.cdc.gov/labstandards/hs_standardization.html. Accessed April 12, 2015.
2. Holick MF, Binkley NC, Bischoff-Ferrare HA, et al. Evaluation, treatment, and prevention of vitamin D deficiency: An Endocrine Society clinical practice guideline. J Clin Endocrinol Metab. 2011; 96(7):1911-1930. 21646368


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
504115 25-Hydroxyvitamin D LCMS D2+D3 62292-8 500815 25-Hydroxy, Vitamin D ng/mL 62292-8
504115 25-Hydroxyvitamin D LCMS D2+D3 62292-8 500816 25-Hydroxy, Vitamin D-2 ng/mL 49054-0
504115 25-Hydroxyvitamin D LCMS D2+D3 62292-8 500817 25-Hydroxy, Vitamin D-3 ng/mL 1989-3

For Providers

Please login to order a test

Order a Test

© 2023 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2023, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf