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Bile Acids, Fractionated and Total, LC/MS-MS

CPT: 82542
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Expected Turnaround Time

5 - 10 days

Related Documents

Specimen Requirements


Serum (preferred) or plasma, frozen


1 mL

Minimum Volume

0.3 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (sodium heparin) tube


Serum/plasma must be separated from cells within two hours of venipuncture. Transfer serum/plasma to a plastic transport tube before freezing.

Storage Instructions

Refrigerate or freeze.

Stability Requirements



Room temperature

1 day


3 days


14 days

Freeze/thaw cycles

Stable x6

Patient Preparation

Patient should be fasting.

Causes for Rejection

Whole blood

Test Details


This assay uses LC/MS-MS to quantify the free acid, glycine-conjugate and taurine-conjugate forms of cholic acid, chenodeoxycholic acid, deoxycholic acid, and ursodeoxycholic acid. Bile acids may be measured to investigate obstructive cholestasis in pregnancy.1 In addition, bile acids are required for cholesterol absorption, fat-soluble vitamin absorption, and to provide resistance to overgrowth of intestinal bacteria.1 Bile acid levels may be reduced or elevated due to various genetic defects.


High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)

Additional Information

Bile acids are formed in the liver from cholesterol, stored and concentrated in the gallbladder, and excreted into the intestines in response to food. The liver synthesizes cholesterol into two primary bile acids, cholic acid and chenodeoxycholic acid. The primary bile acids are converted by intestinal bacteria to the secondary bile acids, deoxycholic acid and lithocholic acid. A fraction of chenodeoxycholic acid is also transformed into the tertiary bile acid, ursodeoxycholic acid.


1. Monte MJ, Marin JJ, Antelo A, Vazquez-Tato J. Bile acids: Chemistry, physiology, and pathophysiology. World J Gastroenterol. 2009; 15(7):804-816.19230041


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
503640 Bile Acids, Fractionated LCMS 503642 Ursodeoxycholic Acids umol/L 55159-8
503640 Bile Acids, Fractionated LCMS 503643 Cholic Acids umol/L 30518-5
503640 Bile Acids, Fractionated LCMS 503644 Chenodeoxycholic Acids umol/L 30519-3
503640 Bile Acids, Fractionated LCMS 503645 Deoxycholic Acids umol/L 30520-1
503640 Bile Acids, Fractionated LCMS 503641 Total Bile Acids umol/L 14628-2

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