Herpes Simplex Virus (HSV) Types 1 and 2-Specific Antibodies, IgG

CPT: 86695; 86696
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  • HSV-1
  • HSV-2

Expected Turnaround Time

1 - 3 days

Related Documents

Specimen Requirements




1.5 mL

Minimum Volume

1.0 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube or serum transfer tube

Storage Instructions


Stability Requirements



Room temperature

2 days


7 days


98 days

Freeze/thaw cycles

Stable x4

Causes for Rejection

Hemolysis; lipemia; grossly icteric; visible particulate matter; gross bacterial contamination

Test Details


This test is used to detect IgG antibodies specific to HSV types 1 and/or 2 infection and confirm or rule out possible infection with herpes simplex types 1 and/or 2 virus in prenatal patients in whom HSV 2 infection can cause serious prenatal disease. In a patient with no history of lesion disease, a positive result for this test may be indicative that the primary infection was asymptomatic. Once infected by HSV, it is possible for a patient to shed the virus without the development of lesions (asymptomatic shedding). This test does not indicate the site of HSV infection. The magnitude of the Index Value is not indicative of the amount of antibody present in the patient sample. There is a considerable homology between HSV-1 and HSV-2 antigens, so that antibodies formed against either virus are highly cross-reactive. These assays are based on purified recombinant glycoprotein G-1 (HSV-1) or G-2 (HSV-2) antigens.


Serum samples collected too early in the course of infection may not have detectable levels of HSV IgG. In cases of suspected early disease, a repeat serum specimen should be collected 14-21 days later and submitted for testing. The predictive value of positive or negative results depends on the prevalence of disease and the pretest likelihood of HSV-1 and HSV-2.

False-positive results may occur. Repeat testing, or testing by a different method, may be indicated in some settings (e.g., patients with low likelihood of HSV infection).

This test is intended for qualitative determination only. The magnitude of the Index Value is not indicative of the amount of antibody present in the patient sample.


Chemiluminescent immunoassay (CLIA)


American Academy of Pediatrics, American College of Obstetricians and Gynecologists. In: Kilpatrick SJ, Papile LA, eds. Guidelines for Perinatal Care, 8th Edition. ElkGrove, IL, and Washington, D.C.; 2017.
Landry LL. Immunoglobulin M for Acute Infection: True or False? Clin Vaccine Immuol. 2016 Jul 5;23(7):540-545.27193039
Miller JM, Binnicker MJ, Campbell S, et al. A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the InfectiousDiseases Society of America and the American Society for Microbiology. Clin Infect Dis. 2018 Aug 31;67(6): e1-e94.29955859
US Preventive Services Task Force, Bibbins-Domingo K, Grossman DC, et al. Serologic Screening for Genital Herpes Infection: US Preventive Services Task ForceRecommendation Statement. JAMA. 2016 Dec 20;316(23):2525-2530.27997659
Workowski KA, Bachmann LH, Chan PH, et al. Sexually Transmitted Infections Treatment Guidelines, 2021. MMWR Recomm Rep. 2021 Jul 23;70(4):1-187.34292926


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164099 HSV 1 and 2 Ab, IgG 27948-9 164903 HSV 1 IgG, Type Spec index 5206-8
164099 HSV 1 and 2 Ab, IgG 27948-9 164097 HSV 2 IgG, Type Spec index 5209-2

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