Antineutrophil Cytoplasmic Antibodies (ANCA)

CPT: 86037(x3)
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Test Includes

Antineutrophil cytoplasmic autoantibodies exhibiting pANCA or cANCA patterns are detected using indirect immunofluorescent antibody (IFA) techniques with a substrate of ethanol-fixed human neutrophils. Positive patient sera with perinuclear or nuclear patterns are repeated using a formalin-fixed substrate in order to differentiate the presence of true pANCA antibodies from possible interference with antinuclear antibodies (ANA).

Expected Turnaround Time

3 - 5 days

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Specimen Requirements




1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross hemolysis; lipemia; bacterial contamination

Test Details


Antineutrophil cytoplasmic autoantibodies (ANCA) are a serological marker associated with vasculitis and glomerulonephritis. Specifically, ANCA have been found (at frequencies ranging from 70% to 90%) in patients with active Wegener granulomatosis, microscopic polyarteritis nodosa, and idiopathic crescentic glomerulonephritis (with manifestations ranging from kidney-limited disease to extrarenal systemic disease, including pulmonary-renal syndromes). Glomerular lesions in patients with ANCA-associated systemic vasculitis or renal-limited disease are virtually identical. Normal controls are negative for ANCA and <10% of patients with other renal diseases are positive. ANCA may be directly involved in the pathogenesis of the vascular injury that causes the clinical manifestations in ANCA-associated disease.


Indirect fluorescent antibody (IFA)

Reference Interval

Negative: <1:20


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
162388 Antineutrophil Cytoplasmic Ab 162400 Cytoplasmic (C-ANCA) titer 14277-8
162388 Antineutrophil Cytoplasmic Ab 162401 Perinuclear (P-ANCA) titer 14278-6
162388 Antineutrophil Cytoplasmic Ab 162402 Atypical pANCA titer 49503-6

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