Antineutrophil Cytoplasmic Antibodies (ANCA)

CPT: 86037(x3)
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Test Includes

Antineutrophil cytoplasmic autoantibodies exhibiting pANCA or cANCA patterns are detected using indirect immunofluorescent antibody (IFA) techniques with a substrate of ethanol-fixed human neutrophils. Positive patient sera with perinuclear or nuclear patterns are repeated using a formalin-fixed substrate in order to differentiate the presence of true pANCA antibodies from possible interference with antinuclear antibodies (ANA).


Expected Turnaround Time

3 - 5 days


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Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Gross hemolysis; lipemia; bacterial contamination


Test Details


Use

Antineutrophil cytoplasmic autoantibodies (ANCA) are a serological marker associated with vasculitis and glomerulonephritis. Specifically, ANCA have been found (at frequencies ranging from 70% to 90%) in patients with active Wegener granulomatosis, microscopic polyarteritis nodosa, and idiopathic crescentic glomerulonephritis (with manifestations ranging from kidney-limited disease to extrarenal systemic disease, including pulmonary-renal syndromes). Glomerular lesions in patients with ANCA-associated systemic vasculitis or renal-limited disease are virtually identical. Normal controls are negative for ANCA and <10% of patients with other renal diseases are positive. ANCA may be directly involved in the pathogenesis of the vascular injury that causes the clinical manifestations in ANCA-associated disease.


Methodology

Indirect fluorescent antibody (IFA)


Reference Interval

Negative: <1:20


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
162388 Antineutrophil Cytoplasmic Ab 162400 Cytoplasmic (C-ANCA) titer 14277-8
162388 Antineutrophil Cytoplasmic Ab 162401 Perinuclear (P-ANCA) titer 14278-6
162388 Antineutrophil Cytoplasmic Ab 162402 Atypical pANCA titer 49503-6

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