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Hepatitis B Surface Antigen (HBsAg) Screen ; Hepatitis B Surface Antibody (anti-HBs) ; Hepatitis B Core Antibody, Total (anti-HBc) .
Reflex of HBsAg + to: HBV quant DNA .
If reflex test is performed, additional charges/CPT codes may apply.
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
1 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Serum or EDTA plasma
2.9 mL (Note: This volume does not allow for repeat testing.)
Gel-barrier tube, red top tube, or lavender-top (EDTA) tube
Two samples must be submitted.
HBsAg, anti-HBs, anti-HBc Total: If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.
HBV DNA: Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT can be frozen in situ.
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.
HBsAg, anti-HBs, anti-HBc Total: Room temperature (preferred) up to 14 days. Refrigerated up to 14 days. Frozen up to 14 days.
HBV DNA: Frozen (preferred) up to 12 weeks. Refrigerated up to 6 days. Room temperature up to 24 hours.
Heat-inactivated specimens; cord blood; cadaveric specimens; or body fluid other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling
Risk assessment and management of HBV infection status in patients prior to initiation of HCV treatment with direct-acting antiviral agents.
The HBV quantitative real-time PCR assay has a quantitative range of 10 to 1,000,000,000 IU/mL.
Immunochemiluminometric assay (ICMA)
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