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For hours, walk-ins and appointments.Synonyms
- Hepatitis
Test Includes
Hepatitis B Surface Antigen (HBsAg). Specimens positive for HBsAg reflex to Hepatitis Be Antigen (HBeAg), Hepatitis Be Antibody (anti-HBe), HBV Quantitative DNA, and ALT.
Special Instructions
If reflex test is performed, additional charges/CPT code(s) may apply.
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Expected Turnaround Time
1 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Serum or EDTA plasma
Volume
9.5 mL (2.5 mL for HBV DNA)
Minimum Volume
3.6 mL (0.7 mL for HBV DNA)
Container
Gel-barrier tube, red top tube, or lavender-top (EDTA) tube
Collection
Two samples must be submitted.
All tests except DNA: If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.
HBV DNA: Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT can be frozen in situ.
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.
Storage Instructions
All tests except DNA: Refrigerated (preferred) up to 7 days. Frozen up to 14 days. Room temperature up to 7 days.
HBV DNA: Frozen (preferred) up to 12 weeks. Refrigerated up to 6 days. Room temperature up to 24 hours.
Causes for Rejection
Heat-inactivated specimens; cord blood; cadaveric specimens; or body fluid other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling
Test Details
Use
This panel is intended for use in the management of patients with chronic hepatitis B and to assess the efficacy of antiviral treatment. A positive HBsAg test will reflex to HBeAg, anti-HBe, HBV quantitative DNA, and ALT.
Limitations
Anti-HBe: Assay performance characteristics have not been established for testing of children less than 17 years of age or pregnant women or in immunocompromized or immunosuppressed patients.
The HBV quantitative real-time PCR assay has a quantitative range of 10 to 1,000,000,000 IU/mL.
Methodology
Immunochemiluminometric assay (ICMA) (see individual reflex tests for methodologies)
References
Abara WE, Oaseem A, Schillie S. McMahon BJ, Harris AM, High Value Care Task Force of the American College of Physicians and the Centers for Disease Control and Prevention. Hepatitis B Vaccination, Screening, and Linkage to Care: Best Practice Advice from the American College of Physicians and the Centers for Disease Control and Prevention. Ann Intern Med. 2017 Dec 5;167(11):794-804.29159414
Centers for Disease Control and Prevention. Hepatitis B. CDC web site: www.cdc.gov/hepatitis/hbv/index.htm. Accessed May 2021.
Schillie S, Vellozzi C, Reingold A, et al. Prevention of Hepatitis B Virus Infection in the United States: Recommendations of the Advisory Committee on Imunization Practices. MMWR Recomm Rep. 2018 Jan 12;67(1):1-31.29939980
Terrault NA, Lok ASF, McMahon BJ, et al. Update on Prevention, Diagnosis, and Treatment of Chronic Hepatitis B: AASLD 2018 Hepatitis B Guidance. Hepatology. 2018 Apr;67(4):1560-1599.29405329
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 144149 | HBV Patient Mgmt w/rflx | 5196-1 | 006510 | HBsAg Screen | 5196-1 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 016105 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 001545 | ALT (SGPT) | IU/L | 1742-6 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 006619 | Hep Be Ag | 13954-3 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 006635 | Hep Be Ab | 13953-5 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551629 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex 2 | 551595 | HBV Real-Time PCR, Quant | 551628 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551629 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex 2 | 551595 | HBV Real-Time PCR, Quant | 551596 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551597 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551719 | Test Information: | 19147-8 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 001545 | ALT (SGPT) | IU/L | 1742-6 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 006619 | Hep Be Ag | 13954-3 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 006635 | Hep Be Ab | 13953-5 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551629 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex 3 | 551595 | HBV Real-Time PCR, Quant | 551628 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551629 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex 3 | 551595 | HBV Real-Time PCR, Quant | 551596 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551597 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex Table for HBsAg Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 144406 | HBsAg Confirmation | 016105 | HBsAg Confirmation | 7905-3 | |
| Reflex 2 | 144236 | ALT+HBeAb+HBeAg+HBV Qn | 551719 | Test Information: | 19147-8 | |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf